Recombinant Human Integrin alpha 6 beta 1 Protein, CF

R&D Systems | Catalog # 7809-A6

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human Integrin alpha 6 beta 1 Protein (7809-A6)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

NP_000201 ( alpha 6 subunit) & P05556 ( beta 1 subunit)

Structure / Form

Noncovalently-linked heterodimer

Applications

Bioactivity
View all Integrin alpha 6 beta 1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha 6 beta 1 protein
Human Integrin alpha 6
(Phe24-Ser1012)
Accession # NP_000201		 GGGSGGGS Acidic Tail HHHHHH
Human Integrin beta 1
(Gln21-Asp728)
Accession # P05556		 GGGSGGGS Basic Tail
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe24 ( alpha 6 subunit) & Gln21 predicted: No results obtained, sequencing might be blocked ( beta 1 subunit)

Predicted Molecular Mass

119 kDa ( alpha 6 subunit) & 86.4 kDa ( beta 1 subunit)

SDS-PAGE

110-140 kDa & 140 - 170 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Laminin alpha 4 (Catalog # 7340-A4) is coated at 5 μg/mL, Recombinant Human Integrin  alpha 6 beta 1 binds with apparent KD <2nM.

Reviewed Applications

Read 2 reviews rated 5 using 7809-A6 in the following applications:

Formulation, Preparation, and Storage

7809-A6
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha 6 beta 1

Integrin alpha 6 beta 1, also called platelet glycoprotein GPIc-IIa, is a laminin binding integrin that is expressed on T cells, monocytes, endothelial cells, stem cells, and platelets (1-9). The non-covalent heterodimer is composed of ~150 kDa alpha 6/CD49f and 130 kDa beta 1/CD29 type I transmembrane glycoprotein subunits (2). While alpha 6 pairs only with beta 1 or beta 4, twelve integrins share the beta 1 subunit (1-5). The alpha 6 subunit is cleaved into extracellular heavy and transmembrane light chains (3). Alternative splicing in the human alpha 6 extracellular domain (ECD) at amino acid (aa) 216 creates X1 (ubiquitous), X2 and X1X2 isoforms, while splicing at a mouse or human cytoplasmic site creates A and B isoforms (10, 11). These forms do not appear to alter the binding specificity (4, 10, 11). The beta 1 ECD contains a vWFA domain, which participates in binding. Each subunit then has a transmembrane sequence and a short cytoplasmic tail. The dimer is folded when it is least active. Divalent cations and intracellular (inside-out) signaling convert it to its most active, extended and open conformation (1, 2). The human alpha 6 (X1) heavy chain shares 94‑95% aa identity with mouse, rat, bovine, and canine alpha 6, and the human beta 1 ECD shares 92‑96% aa sequence identity with rat, bovine, mouse, and feline beta 1. alpha 6 beta 1 shows broad specificity for adhesion to laminin isoforms (4, 10). Its expression on human and mouse pluripotent stem cells is important for attachment, expansion, and self‑renewal on LN‑511 (laminin  alpha 5  beta 1 gamma 1) (6, 7). The secreted protein Netrin-4 and the laminin  gamma 1 subunit form an adhesion‑activating complex with alpha 6 beta 1 on mouse neural stem cells and human lymphatic endothelial cells that promotes lymphangiogenesis (8, 9). alpha 6 beta 1 up‑regulation on cancers such as prostate, glioma, and hepatoma is reported to enhance tumorigenicity, motility, invasion and metastasis (12‑14). alpha 6 beta 1 cleavage via uPA (urokinase-type plasminogen activator) facilitates tumorigenicity in prostate cancers, and interaction of hepatoma alpha 6 beta 1 with EMMPRIN/CD147 may also enhance tumorigenicity by inducing uPA and other metalloproteinases (12, 13).

References

  1. Takada, Y. et al. (2007) Genome Biol. 8:215.
  2. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
  3. Tamura, R.N. et al. (1990) J. Cell Biol. 111:1593.
  4. Nishiuchi, R. et al. (2006) Matrix Biol. 25:189.
  5. Sonnenberg, A. and C.J.T. Linders (1990) J. Cell Science 96:207.
  6. Rodin, S. et al. (2010) Nat. Biotech. 28:611.
  7. Domogatskaya A. et al. (2008) Stem Cells 26:2800.
  8. Staquicini, F.I. et al. (2009) Proc. Natl. Acad. Sci. USA 106:2903.
  9. Larrieu-Lahargue, F. et al. (2011) Circ. Res. 109:770.
  10. Delwel, G. O. et al. (1995) Cell Adhes. Commun. 3:143.
  11. Hogervorst, F. et al. (1993) J. Cell Biol. 121:179.
  12. Sroka, I.C. et al. (2011) Mol. Cancer Res. 9:1319.
  13. Dai, J.Y. et al. (2009) BMC Cancer 9:337.
  14. Delamarre, E. et al. (2009) Am. J. Pathol. 175:844.

Alternate Names

CD49f, ITGA6B, VLA-6

UniProt

NP_000201 ( alpha 6 subunit) & P05556 ( beta 1 subunit)

Additional Integrin alpha 6 beta 1 Products

Product Documents for Recombinant Human Integrin alpha 6 beta 1 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Integrin alpha 6 beta 1 Protein, CF

For research use only

Citations for Recombinant Human Integrin alpha 6 beta 1 Protein, CF

Customer Reviews for Recombinant Human Integrin alpha 6 beta 1 Protein, CF (2)

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FAQs for Recombinant Human Integrin alpha 6 beta 1 Protein, CF

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  • Q: What is the amino acid sequence of the acidic and basic tails?

    A: Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.

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