Recombinant Human Latent TGF-beta bp4 Protein, CF

Catalog # Availability Size / Price Qty
8068-LT-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human Latent TGF-beta bp4 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE with silver staining
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of HFL1 human fetal lung fibroblast cells. The ED50 for this effect is 0.5-2.5 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human Latent TGF-beta bp4 protein
Met1-Ala1624, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Multiple starts between Met1 & Ser273
Predicted Molecular Mass
174 kDa
SDS-PAGE
155-205 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8068-LT

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

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Background: Latent TGF-beta bp4

Latent TGF‑ beta Binding Protein‑4 (LTBP‑4) is a widely expressed 170‑230 kDa secreted glycoprotein within the LTBP/fibrillin family (1‑3). LTBPs contain a conserved pattern of eight-cysteine motifs (8Cys) and EGF‑like (EGFL) domains (2‑4). The 1624 amino acid (aa) human LTBP‑4 (L isoform) includes a signal sequence (aa 1‑27), a heparin-binding N‑terminal domain (aa 28‑460) with two EGFL, one hybrid and one 8‑Cys motif, a protease-sensitive hinge region, a central region with 10 EGFL motifs, and a C‑terminal domain (aa 1180‑1624) with two EGFL and two 8Cys (1‑4). The first C‑terminal 8Cys motif is responsible for covalent association with the latency associated peptide (LAP) of latent TGF‑ beta 1 (1‑4). Unlike LTBP-1 and ‑3, LTBP‑4 does not bind TGF‑ beta 2 or TGF‑ beta 3 LAPs (4). LTBP‑4 also binds fibronectin, which mediates targeting to extracellular matrix (ECM), fibrillin-1 which mediates binding to microfibrils, and the cytokine midkine (1, 5, 6). Mature human LTBP‑4 (LTBP‑4L) shares 87% and 90% aa sequence identity with mouse and rat LTBP‑4, respectively. A variant that lacks the TGF‑binding 8Cys motif is reported to be widely expressed along with LTBP‑4L (3). Other differentially expressed human splice isoforms of 1557 (LTBP‑4S), 1587 and 636 aa have alternate N‑termini through aa 69, 151, and 919 (also lacking aa 1005‑1090), respectively (1, 7). LTBP‑4 is involved in the assembly, secretion and targeting of latent TGF‑ beta 1 to sites where it can be activated. Deletion or mutation in humans can cause Urban‑Rifkin‑Davis syndrome (URDS), a syndrome of impaired pulmonary, gastrointestinal, genitourinary, musculoskeletal and dermal development mediated by enhanced TGF‑ beta 1 activity (8). Similar defects are found in mice deleted for LTBP‑4 (9‑11). Impaired LTBP‑4 expression enhances susceptibility to colon cancer (8). LTBP-4 is also down‑regulated in breast cancer tissues (5).

References
  1. Kantola, A.K. et al. (2008) Exp. Cell Res. 314:2488.
  2. Saharinen, J. et al. (1998) J. Biol. Chem. 273:18459.
  3. Koli, K. et al. (2001) J. Cell Sci. 114:2869.
  4. Saharinen, J. and J. Keski-Oja (2000) Mol. Biol. Cell 11:2691.
  5. Takahashi, Y. et al. (2012) Breast Cancer 19:46.
  6. Yan, Q. et al. (2012) Hepatobiliary Pancreat. Dis. Int. 11:272.
  7. Kantola, A.K. et al. (2010) J. Cell Physiol. 223:727.
  8. Urban, Z. et al. (2009) Am. J. Hum. Genet. 85:593.
  9. Sterner-Kock, A. et al. (2002) Genes Dev. 16:2264.
  10. Dabovic, B. et al. (2009) J. Cell Physiol. 219:14.
  11. Koli, K. et al. (2004) J. Cell Biol. 167:123.
Entrez Gene IDs
8425 (Human)
Alternate Names
Latent TGFbeta bp4; Latent TGF-beta bp4; latent transforming growth factor beta binding protein 4; LTBP4; LTBP-4; LTBP4L; LTBP4S

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