Recombinant Human LILRB2/CD85d/ILT4 His-tag Avi Protein, CF

Biotinylated (HEK293-expressed)
Catalog # Availability Size / Price Qty
AVI8429-050

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Biotinylated Recombinant Human LILRB2/CD85d/ILT4 His-tag Avi-tag Protein Binding Activity.
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Recombinant Human LILRB2/CD85d/ILT4 His-tag Avi Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Biotinylated Recombinant Human LILRB2/CD85d/ILT4 His-tag Avi-tag (Catalog # AVI8429) is in presented at 2 μg/mL (100 µL/well), the concentration of Recombinant Human Angiopoietin-like 7 Protein (Catalog # 914-AN) that produces 50% of the optimal binding response is 0.100-0.800 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human LILRB2/CD85d/ILT4 protein
Human LILRB2/CD85d/ILT4
(Gln22-His458)
Accession # Q8N423.4
6-His tagAvi-tag
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Gln22 inferred from enzymatic pyroglutamate treatment revealing Thr23
Structure / Form
Biotinylated via Avi-tag
Predicted Molecular Mass
50 kDa
SDS-PAGE
65-75 kDa, under reducing conditions.

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AVI8429

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI8429

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Biotinylated Recombinant Human LILRB2/CD85d/ILT4 His-tag Avi-tag Protein Binding Activity. View Larger

When Biotinylated Recombinant Human LILRB2/CD85d/ILT4 His-tag Avi-tag (Catalog # AVI8429) is in presented at 2 μg/mL (100 µL/well), the concentration of Recombinant Human Angiopoietin-like 7 Protein (914-AN) that produces 50% of the optimal binding response is 0.100‑0.800 μg/mL.

SDS-PAGE Biotinylated Recombinant Human LILRB2/CD85d/ILT4 His-tag Avi-tag Protein SDS-PAGE. View Larger

2 μg/lane of Biotinylated Recombinant Human LILRB2/CD85d/ILT4 His-tag Avi-tag Protein (Catalog # AVI8429) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 65-75 kDa.

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Background: LILRB2/CD85d/ILT4

Immunoglobulin-like transcript 4 (ILT4), also known as Leukocyte immunoglobulin-like receptor subfamily B member 2 (LILRB2) and LIR2, is a member of a family of activating and inhibitory type immunoreceptors mainly expressed in myeloid cells. The LILRB family contains five members, LILRB1–5, with a varying number of Ig‑like domains in their extracellular domains (ECD) that play roles in human immunity and are considered immune checkpoint factors (1). Mature human ILT4 consists of an ECD with 4 Ig‑like domains, a transmembrane segment, and a cytoplasmic domain with 3 inhibitory immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The ECD of human ILT4 shares 76% amino acid sequence identity with chimpanzee ILT4. While relatives of ILT4s exist in birds and mammals, ILT4 homologs are not found in lower organisms (2). ILT4 is primarily expressed by monocytes, macrophages, and dendritic cells and binds to classical MHC I proteins as well as the non-classical HLA-G1 and HLA-F molecules (3,4). ILT4 appears to modulate immune responses during mid- and late-activation phases of the neutrophil lifecycle. ILT4 activation promotes the development of tolerogenic dendritic cells and the subsequent induction of regulatory T cells and CD4+ T cell anergy (5). ILT4 is also highly expressed in various solid tumors and is a potent driver for tumor growth, invasion and metastasis, and blocking ILT4 in tumor cells might be an effective strategy for cancer therapy (6). Our Avi-tag Biotinylated ILT4 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References
  1. Kang, X. et al. (2016) Cell cycle 15:25.
  2. Dennis, G. et al. (2000) Proc. Natl. Acad. Sci. U.S.A. 97:13245.
  3. Baudhuin, J. et al. (2013) Proc. Natl. Acad. Sci. U.S.A. 110:17957.
  4. Shiroishi, M. et al. (2003) Proc. Natl. Acad. Sci. U.S.A. 100:8856.
  5. Wu, J. and Horuzsko, A. (2009) Human immunology 70:353.
  6. Chen, H.M. et al. (2018) J. Clin. Invest. 128:5647.
Long Name
Leukocyte Immunoglobulin-like Receptor, Subfamily B (with TM and ITIM Domains), Member 5
Entrez Gene IDs
10288 (Human)
Alternate Names
CD85 antigen-like family member D; CD85d antigen; CD85d; Ig-like transcript 4; ILT4; ILT-4; ILT4CD85d; Immunoglobulin-like transcript 4; Leukocyte immunoglobulin-like receptor 2; leukocyte immunoglobulin-like receptor subfamily B member 2 soluble isoform; leukocyte immunoglobulin-like receptor subfamily B member 2; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRB2; LIR2; LIR2CD85D; LIR-2subfamily A (with TM domain), member 6; member 2; MIR10; MIR10LILRA6; Monocyte/macrophage immunoglobulin-like receptor 10

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