Recombinant Human LILRB2/CD85d/ILT4 His-tag Avi Protein, CF

R&D Systems | Catalog # AVI8429

Biotinylated (HEK293-expressed)
R&D Systems
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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human LILRB2/CD85d/ILT4 His-tag Avi Protein (AVI8429)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

Q8N423.4

Structure / Form


Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity
View all LILRB2/CD85d/ILT4 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human LILRB2/CD85d/ILT4 protein
Human LILRB2/CD85d/ILT4
(Gln22-His458)
Accession # Q8N423.4		 6-His tag Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln22 inferred from enzymatic pyroglutamate treatment revealing Thr23

Predicted Molecular Mass

50 kDa

SDS-PAGE

65-75 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Biotinylated Recombinant Human LILRB2/CD85d/ILT4 His-tag Avi-tag (Catalog # AVI8429) is captured on EvenCoat Streptavidin Coated Plates (Catalog # CP004) at 2 μg/mL (100 µL/well), the concentration of Recombinant Human Angiopoietin-like 7 Protein  (Catalog # 914-AN) that produces 50% of the binding response is 0.100-0.800 μg/mL.

Scientific Data Images for Recombinant Human LILRB2/CD85d/ILT4 His-tag Avi Protein, CF

Biotinylated Recombinant Human LILRB2/CD85d/ILT4 His-tag Avi-tag Protein Binding Activity.

When Biotinylated Recombinant Human LILRB2/CD85d/ILT4 His-tag Avi-tag (Catalog # AVI8429) is captured on EvenCoat Streptavidin Coated Plates (CP004) at 2 μg/mL (100 µL/well), the concentration of Recombinant Human Angiopoietin-like 7 Protein (914-AN) that produces 50% of the optimal binding response is 0.100-0.800 μg/mL.

Biotinylated Recombinant Human LILRB2/CD85d/ILT4 His-tag Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human LILRB2/CD85d/ILT4 His-tag Avi-tag Protein (Catalog # AVI8429) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 65-75 kDa.

Formulation, Preparation, and Storage

AVI8429
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LILRB2/CD85d/ILT4

Immunoglobulin-like transcript 4 (ILT4), also known as Leukocyte immunoglobulin-like receptor subfamily B member 2 (LILRB2) and LIR2, is a member of a family of activating and inhibitory type immunoreceptors mainly expressed in myeloid cells. The LILRB family contains five members, LILRB1–5, with a varying number of Ig‑like domains in their extracellular domains (ECD) that play roles in human immunity and are considered immune checkpoint factors (1). Mature human ILT4 consists of an ECD with 4 Ig‑like domains, a transmembrane segment, and a cytoplasmic domain with 3 inhibitory immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The ECD of human ILT4 shares 76% amino acid sequence identity with chimpanzee ILT4. While relatives of ILT4s exist in birds and mammals, ILT4 homologs are not found in lower organisms (2). ILT4 is primarily expressed by monocytes, macrophages, and dendritic cells and binds to classical MHC I proteins as well as the non-classical HLA-G1 and HLA-F molecules (3,4). ILT4 appears to modulate immune responses during mid- and late-activation phases of the neutrophil lifecycle. ILT4 activation promotes the development of tolerogenic dendritic cells and the subsequent induction of regulatory T cells and CD4+ T cell anergy (5). ILT4 is also highly expressed in various solid tumors and is a potent driver for tumor growth, invasion and metastasis, and blocking ILT4 in tumor cells might be an effective strategy for cancer therapy (6). Our Avi-tag Biotinylated ILT4 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Kang, X. et al. (2016) Cell cycle 15:25.
  2. Dennis, G. et al. (2000) Proc. Natl. Acad. Sci. U.S.A. 97:13245.
  3. Baudhuin, J. et al. (2013) Proc. Natl. Acad. Sci. U.S.A. 110:17957.
  4. Shiroishi, M. et al. (2003) Proc. Natl. Acad. Sci. U.S.A. 100:8856.
  5. Wu, J. and Horuzsko, A. (2009) Human immunology 70:353.
  6. Chen, H.M. et al. (2018) J. Clin. Invest. 128:5647.

Long Name

Leukocyte Immunoglobulin-like Receptor, Subfamily B (with TM and ITIM Domains), Member 5

Alternate Names

CD85d, ILT4, LIR2, MIR10

Entrez Gene IDs

10288 (Human)

Gene Symbol

LILRB2

UniProt

Q8N423.4

Additional LILRB2/CD85d/ILT4 Products

Product Documents for Recombinant Human LILRB2/CD85d/ILT4 His-tag Avi Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human LILRB2/CD85d/ILT4 His-tag Avi Protein, CF

For research use only

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