Recombinant Human Thrombopoietin (E. coli-expressed), CF

R&D Systems | Catalog # 288-TPE

GMP Version Available: BT-TPO-GMP
GMP
New Version Available: BT-TPO
NEW
R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems E. coli-derived Recombinant Human Thrombopoietin (E. coli-expressed) (288-TPE)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

NP_000451

Applications

Bioactivity
View all Thrombopoietin/Tpo Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Thrombopoietin/Tpo protein
Ser22-Leu195

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala-Ser22

Predicted Molecular Mass

18.7 kDa

SDS-PAGE

19 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359.
The ED50 for this effect is 0.05-0.5 ng/mL.

Scientific Data Images for Recombinant Human Thrombopoietin (E. coli-expressed), CF

Recombinant Human Thrombopoietin (E. coli-expressed) Bioactivity

Recombinant Human Thrombopoietin (E. coli-expressed) Bioactivity

Recombinant Human Thrombopoietin/Tpo (Catalog # 288-TPE) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line. The ED50 for this effect is 0.05-0.5 ng/mL, which is more than 2-fold more active than two competitors'.
Recombinant Human Thrombopoietin (E. coli-expressed) SDS-PAGE

Recombinant Human Thrombopoietin (E. coli-expressed) SDS-PAGE

2 μg/lane of Recombinant Human Thrombopoietin/Tpo was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 19 kDa and 18 kDa, respectively.

Formulation, Preparation, and Storage

288-TPE
Formulation Lyophilized from a 0.2 μm filtered solution in Sodium Acetate.
Reconstitution Reconstitute at 100 μg/mL in sterile, deionized water.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Thrombopoietin/Tpo

Thrombopoietin (Tpo), is a key regulator of megakaryocytopoiesis and thrombopoiesis. It is principally produced in the liver and is bound and internalized by the receptor Tpo R/c-mpl. Defects in the Tpo-Tpo R signaling pathway are associated with a variety of platelet disorders (1-3). The 353 amino acid (aa) human Tpo precursor is cleaved to yield the 332 aa mature protein. Mature human Tpo shares approximately 70% aa sequence homology with mouse and rat Tpo. It is an 80‑85 kDa protein that consists of an N‑terminal domain with homology to Erythropoietin (Epo) and a C‑terminal domain that contains multiple N‑linked and O-linked glycosylation sites (4, 5). Tissue specific alternate splicing of human Tpo generates multiple isoforms with internal deletions, insertions, and/or C‑terminal substitutions (6). Tpo promotes the differentiation, proliferation, and maturation of MK and their progenitors (4, 5, 7). Several other cytokines can promote these functions as well but only in cooperation with Tpo (8, 9). Notably, IL-3 independently induces MK development, although its effects are restricted to early in the MK lineage (8, 9). Tpo additionally promotes platelet production, aggregation, ECM adhesion, and activation (10-13). It is cleaved by platelet-derived thrombin following Arg191 within the C‑terminal domain and subsequently at other sites upon extended digestion (14). Both full length Tpo and shorter forms circulate in the plasma, with the shorter, N‑terminal EPO-like domain forms showing significantly increased specific activity (4, 5, 15). The C‑terminal domain is not required for binding to Tpo R or inducing MK growth and differentiation (5). Aside from its hematopoietic effects, Tpo is expressed in the brain where it promotes the apoptosis of hypoxia-sensitized neurons and inhibits neuronal differentiation by blocking NGF induced signaling (16, 17).

References

  1. Deutsch, V.R. and A. Tomer (2006) Br. J. Haematol. 134:453.
  2. Kaushansky, K. (2005) J. Clin. Invest. 115:3339.
  3. Li, J. et al. (1999) Br. J. Haematol. 106:345.
  4. Bartley, T.D. et al. (1994) Cell 77:1117.
  5. de Sauvage, F.J. et al. (1994) Nature 369:533.
  6. Marcucci, R. and M. Romano (2008) Biochim. Biophys. Acta 1782:427.
  7. Kaushansky, K. et al. (1994) Nature 369:568.
  8. Kaushansky, K. et al. (1995) Proc. Natl. Acad. Sci. 92:3234.
  9. Broudy, V.C. et al. (1995) Blood 85:1719.
  10. Lok, S.I. et al. (1994) Nature 369:565.
  11. Chen, J. et al. (1995) Blood 86:4054.
  12. Oda, A. et al. (1996) Blood 87:4664.
  13. Van Os, E. et al. (2003) Br. J. Haematol. 121:482.
  14. Kato, T. et al. (1997) Proc. Natl. Acad. Sci. 94:4669.
  15. Foster, D. & Hunt, P. (1997) Thrombopoiesis and Thrombopoietins 13:203.
  16. Ehrenreich, H. et al. (2005) Proc. Natl. Acad. Sci. 102:862.
  17. Samoylenko, A. et al. (2008) Cell. Signal. 20:154.

Alternate Names

MGDF, MK-CSF, MKCSF, MPLLG, THCYT1, THPO, Tpo

Entrez Gene IDs

7066 (Human); 21832 (Mouse); 81811 (Rat)

Gene Symbol

THPO

UniProt

NP_000451

Additional Thrombopoietin/Tpo Products

Product Documents for Recombinant Human Thrombopoietin (E. coli-expressed), CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human Thrombopoietin (E. coli-expressed), CF

For research use only

Citations for Recombinant Human Thrombopoietin (E. coli-expressed), CF

Customer Reviews for Recombinant Human Thrombopoietin (E. coli-expressed), CF

There are currently no reviews for this product. Be the first to review Recombinant Human Thrombopoietin (E. coli-expressed), CF and earn rewards!

Have you used Recombinant Human Thrombopoietin (E. coli-expressed), CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs for Recombinant Human Thrombopoietin (E. coli-expressed), CF

Showing  1 - 1 of 1 FAQ Showing All

  • Q: What are the differences between the Recombinant Human Thrombopoietin/Tpo Proteins (Catalog #s 288-TP, 288-TPN, and 288-TPE)?

    A: Each of these proteins is produced using a different expression system. 288-TP is Spodoptera frugiperda, Sf21 baculovirus-derived; 288-TPN is mouse myeloma cell line, NS0-derived; and 288-TPE is E. coli-derived.

    Additionally, the sequence length also varies. 288-TP and 288-TPN begin at Ser22 and end at Gly353. 288-TPE also begins at Ser22 but is truncated at the C-terminus, ending at Leu195. Truncation of the C-terminus has been shown to enhance the biological activity of Tpo. 

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Proteins and Enzymes