Recombinant Human MIA Protein, CF

Catalog # Availability Size / Price Qty
9250-IA-100
Recombinant Human MIA Protein, CF Bioactivity
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Recombinant Human MIA Protein, CF Summary

Purity
>95%, by SDS-PAGE with silver staining, under reducing conditions.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Fibronectin Fragment 4 (Catalog # 3624-FN) is immubilized at 1 μg/mL, 100 μL/well, Recombinant Human MIA binds with a typical ED50 of 1-6 μg/mL.
Source
E. coli-derived human MIA protein
Gly25-Gln131, with an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met, Gly25
Predicted Molecular Mass
12 kDa
SDS-PAGE
10 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9250-IA

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and Trehalose.
Reconstitution Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity View Larger

When Recombinant Human Fibronectin Fragment 4 (Catalog # 3624-FN) is coated at 1 μg/mL, Recombinant Human MIA (Catalog # 9250-IA) binds with a typical ED50of 1-6 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MIA

Melanoma Inhibiting Activity (MIA), also known as cartilage-derived retinoic acid-sensitive protein (CD-RAP), is an approximately 11-15 kDa protein that is secreted as a noncovalent homodimer and is structurally related to OTOR/Otoraplin and MIA-2 (1). Mature human MIA contains a SH3 domain and shares 90% and 92% amino acid sequence identity with mouse and rat MIA, respectively (2). Alternative splicing generates a short isoform that lacks the SH3 domain (3). MIA is widely expressed in developing and regenerating cartilage and in the endothelium and parenchyma of developing lungs (4). MIA disrupts cellular interactions with the extracellular matrix by binding to Integrins  alpha 4 beta 1 and alpha 5 beta 1 (5). It competes with Fibronectin fragments for Integrin binding and interferes with Integrin signaling (5). It also functions as a chemoattractant for mesenchymal stem cells and enhances their BMP-2 and TGF-beta 3 induced differentiation into chondrocytes [tscheud]. MIA-deficient mice exhibit delayed chondrocyte differentiation but enhanced chondrocyte proliferation and cartilage repair (7). MIA is up-regulated in several cancers including malignant melanoma, lung adenoma, metastatic oral squamous cell carcinoma, neurofibromatosis type 1 (NF-1)-related tumors, and pancreatic cancer (2, 4, 8-10). It is selectively secreted and internalized from the trailing pole of migrating cells (11, 12). This polarization reduces cellular attachment to the matrix at the trailing pole and contributes to directional tumor cell migration (2, 10, 13, 14).

References
  1. Schmidt, J. et al. (2013) Histol. Histopathol. 28:421.
  2. Blesch, A. et al. (1994) Cancer Res. 54:5695.
  3. Hau, P. et al. (2002) J. Invest. Dermatol. 119:562.
  4. Lin, S. et al. (2008) Dev. Biol. 316:441.
  5. Bauer, R. et al. (2006) J. Biol. Chem. 281:11669.
  6. Tscheudschilsuren, G. et al. (2006) Exp. Cell Res. 312:63.
  7. Schmid, R. et al. (2010) Cell Death Dis. 1:e97.
  8. Marr, D.G. et al. (2004) Int. J. Oncol. 25:105.
  9. Sasahira, T. et al. (2010) Eur. J. Cancer 46:2285.
  10. El Fitori, J. et al. (2005) Cancer Cell Int. 5:3.
  11. Schmidt, J. et al. (2010) Cell Res. 20:1224.
  12. Schmidt, J. and A.K. Bosserhoff (2009) Int. J. Cancer 125:1587.
  13. Poser, I. et al. (2004) Oncogene 23:6115.
  14. Guba, M. et al. (2000) Brit. J. Cancer 83:1216.
Long Name
Melanoma Inhibitory Activity
Entrez Gene IDs
8190 (Human); 12587 (Mouse)
Alternate Names
CD-RAP; Melanoma inhibitory activity protein; melanoma inhibitory activity; melanoma-derived growth regulatory protein; MIA

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Recombinant Human MIA Protein, CF
By Anonymous on 05/22/2019
Application: Immunoassay Standard

Used as calibrator in sandwich assay with AF2050 as both the capture and detection.