Recombinant Human MUC-1 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10332-MU-050
Recombinant Human MUC-1 Fc Chimera Protein, CF Binding Activity
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Recombinant Human MUC-1 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human MUC-1 Fc Chimera (Catalog # 10332-MU) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human Siglec‑9 Fc Chimera (Catalog # 1139-SL) binds with an ED50 of 0.2-1.4 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human MUC-1 protein
Human MUC-1
(Ser24-Ser380)
Accession # P15941-1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Ser24
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
60 kDa
SDS-PAGE
157-192 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10332-MU

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant Human MUC-1 Fc Chimera (Catalog # 10332-MU) is immobilized at 1 μg/mL (100 μL/well), Recombinant Human Siglec‑9 Fc Chimera (Catalog # 1139-SL) binds with an ED50 of 0.2-1.4 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human MUC-1 Fc Chimera (Catalog # 10332-MU) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 157-192 kDa and 310-380 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MUC-1

MUC-1 (Mucin-1) is a type 1 transmembrane glycoprotein that is normally expressed on the apical surface of most epithelial cells (1-2). It is known to be overexpressed by various human carcinomas and is shed into circulation (2). The extracellular domain is made up of tandem repeats (TRs) of 20 aa each, with each TR containing five potential O-glycosylation sites (3). The number of TRs vary between 25-100, depending on the allele (3). Within the mature region including 16 TRs (residues 24-380), human MUC-1 shares 30% aa sequence identity with mouse and rat MUC-1. It has been reported that high expression level of MUC-1 generally correlates with increased mortality rates (4). In addition, MUC-1 is aberrantly underglycosylated on cancer cells with short and sialylated O-linked glycans in contrast to the long, branched chain seen in normal epithelial cells (4-7). It has been demonstrated that MUC-1 can interact with E-selectin and ICAM-1 to mediate firm adhesion of circulating tumor cells and subsequent extravasation in the metastatic adhesion cascade (4). Furthermore, MUC-1 can modulate the tumor immunological microenvironment through engagement of Siglec-9 by inducing the recruitment of beta-catenin to the cytoplasmic tail of MUC-1, increasing the expression of PD-L1 by macrophages, and activating the MEK-ERK pathway (5,6). MUC-1 can also interact with Galectin-3 to promote EGFR activation thus regulating EGFR-associated tumorigenesis and cancer progression (7).

References
  1. Rughetti, A. et al. (2005) J. Immunol. 174:7764.
  2. Engelstaedter, V. et al. (2012) BMC Cancer 12:600.
  3. Taylor-Papadimitriou, J. et al. (1999) Biochim. Biophys. Acta 1455:301.
  4. Geng, Y. et al. (2012) Front Oncol. 2:76.
  5. Tanida, S. et al. (2013) J Biol Chem. 288:31842.
  6. Beatson, R. et al. (2016) Nat Immunol. 17:1273.
  7. Piyush, T. et al. (2017) Cell Death Differ. 24:1937.
Long Name
Mucin 1, Cell Surface-associated
Entrez Gene IDs
4582 (Human)
Alternate Names
Breast carcinoma-associated antigen DF3; Carcinoma-associated mucin; CD227 antigen; CD227; DF3 antigen; EMA; Episialin; H23 antigen; H23AG; KL-6; MAM6; MUC-1; MUC1/ZD; mucin 1, cell surface associated; mucin 1, transmembrane; Mucin-1; Peanut-reactive urinary mucin; PEM; PEMMUC-1/SEC; PEMT; Polymorphic epithelial mucin; PUMMUC-1/X; tumor associated epithelial mucin; Tumor-associated epithelial membrane antigen; Tumor-associated mucin

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