Recombinant Human N-Cadherin Fc Chimera Protein, CF

Analyzed by SEC-MALS
Catalog # Availability Size / Price Qty
1388-NC-050
Recombinant Human N‑Cadherin Fc Chimera Protein SEC-MALS.
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Citations (11)
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Recombinant Human N-Cadherin Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of U‑118‑MG human glioblastoma/astrocytoma cells. Recombinant Human N-Cadherin Fc Chimera immobilized at 10 µg/mL, 100 µL/well, will induce  ≥55% cell adhesion.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human N-Cadherin protein
Human N-Cadherin
Asp160-Ala724
Accession # P19022.4
IEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Asp160
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
89.2 kDa (monomer)
SDS-PAGE
115‑130 kDa, reducing conditions

Product Datasheets

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1388-NC

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1388-NC

Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate.
Reconstitution Reconstitute at 100 μg/mL in sterile, deionized water containing 2 mM calcium 24 hours prior to use.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

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Recombinant Human N-Cadherin Fc Chimera (Catalog # 1388-NC) has a molecular weight (MW) of 244.7 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).

Reconstitution Calculator

Reconstitution Calculator

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Background: N-Cadherin

Neuronal Cadherin (N-Cadherin or NCAD), also known as Cadherin-2 (CDH2), is a 130 kDa type I membrane protein belonging to the Cadherin superfamily of calcium-dependent adhesion molecules. Cadherins are involved in multiple processes including embryonic development, cell migration, and maintenance of epithelial integrity (1, 2). Human N‑Cadherin is synthesized with a 25 amino acid (aa) signal peptide and a 134 aa N‑terminal propeptide. The mature cell surface‑expressed protein consists of a 565 amino acid (aa) extracellular domain (ECD) that contains five Cadherin repeats, a 21 aa transmembrane segment, and a 161 aa cytoplasmic domain (3). Within the ECD, human N-Cadherin shares 98% aa sequence identity with mouse and rat N-Cadherin. In the nervous system, N-Cadherin mediates adhesion between the opposing faces of developing neuronal synapses and between Schwann cells and neuronal axons (4, 5). It interacts in cis or in trans homophilically and with the GluR2 subunit of neuronal AMPA receptors (1, 6). During synaptic maturation, its expression is lost from inhibitory terminals but maintained at excitatory terminals (5). ADAM10-mediated shedding of the N-Cadherin ECD alters cell-cell adhesion, synaptic development, and AMPA receptor activity (7, 8). N-Cadherin can also be cleaved at multiple additional sites within the intracellular or extracellular domains by Calpain, gamma ‑Secretase, and several MMPs (9-13). Cleavage of N‑Cadherin in atherosclerotic plaques contributes alternatively to vascular smooth muscle cell proliferation (MMP-9 and -12) or apoptosis
(MMP‑7) (12, 13). Aberrant cell surface expression of the pro and mature forms of N-Cadherin in cancer results in increased tumor progression and invasiveness (14, 15). N-Cadherin also mediates the adhesion between hematopoeitic progenitor cells and mesenchymal stromal cells of the bone marrow (16).

References
  1. Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
  2. Gumbiner, B.M. (2005) Nat. Rev. Mol. Cell Biol. 6:622.
  3. Reid, R.A. and J.J. Hemperly (1990) Nucleic Acids Res. 18:5896.
  4. Wanner, I.B. and P.M. Wood (2002) J. Neurosci. 22:4066.
  5. Benson, D.L. and H. Tanaka (1998) J. Neurosci. 18:6892.
  6. Saglietti, L. et al. (2007) Neuron 54:461.
  7. Reiss, K. et al. (2005) EMBO J. 24:742.
  8. Malinverno, M. et al. (2010) J. Neurosci. 30:16343.
  9. Jang, Y.-N. et al. (2009) J. Neurosci. 29:5974.
  10. Uemura, K. et al. (2006) Neurosci. Lett. 402:278.
  11. Hartland, S.N. et al. (2009) Liver Int. 29:966.
  12. Williams, H. et al. (2010) Cardiovasc. Res. 87:137.
  13. Dwivedi, A. et al. (2009) Cardiovasc. Res. 81:178.
  14. Maret, D. et al. (2010) Neoplasia 12:1066.
  15. Tanaka, H. et al. (2010) Nat. Med. 16:1414.
  16. Wein, F. et al. (2010) Stem Cell Res. 4:129.
Long Name
Neural Cadherin
Entrez Gene IDs
1000 (Human); 12558 (Mouse); 83501 (Rat)
Alternate Names
ACOGS; ARVD14; cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; calcium-dependent adhesion protein, neuronal; CD325 antigen; CD325; CDH2; CDHN; CDw325; NCAD; N-cadherin 1; NCadherin; N-Cadherin; Neural cadherin; neural-cadherin

Citations for Recombinant Human N-Cadherin Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Targeting breast and pancreatic cancer metastasis using a dual-cadherin antibody
    Authors: DS Micalizzi, D Che, BT Nicholson, JF Edd, N Desai, ER Lang, M Toner, S Maheswaran, DT Ting, DA Haber
    Proceedings of the National Academy of Sciences of the United States of America, 2022-10-18;119(43):e2209563119.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot Control
  2. Telmisartan anti-cancer activities mechanism through targeting N-cadherin by mimicking ADH-1 function
    Authors: M Khorsand, S Khajeh, M Eslami, N Nezafat, Y Ghasemi, V Razban, Z Mostafavi-
    Journal of Cellular and Molecular Medicine, 2022-02-27;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Stabilization of desmoglein-2 binding rescues arrhythmia in arrhythmogenic cardiomyopathy
    Authors: C Schinner, BM Erber, S Yeruva, A Schlipp, V Rötzer, E Kempf, S Kant, RE Leube, TD Mueller, J Waschke
    JCI Insight, 2020-05-07;5(9):.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Bioassay
  4. Dynamic cellular phynotyping defines specific mobilization mechanisms of human hematopoietic stem and progenitor cells induced by SDF1? versus synthetic agents
    Authors: C Monzel, AS Becker, R Saffrich, P Wuchter, V Eckstein, AD Ho, M Tanaka
    Sci Rep, 2018-01-30;8(1):1841.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. N-Cadherin, a novel and rapidly remodelling site involved in vasoregulation of small cerebral arteries
    Authors: Zhe Sun
    J. Physiol. (Lond.), 2017-02-07;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  6. Cadherin engagement improves insulin secretion of single human beta-cells.
    Authors: Parnaud G, Lavallard V, Bedat B, Matthey-Doret D, Morel P, Berney T, Bosco D
    Diabetes, 2014-10-02;64(3):887-96.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Adhesion Assay
  7. MMP20 modulates cadherin expression in ameloblasts as enamel develops.
    Authors: Guan X, Bartlett J
    2013-09-25;92(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Distinct PTPmu-associated signaling molecules differentially regulate neurite outgrowth on E-, N-, and R-cadherin.
    Authors: Oblander SA, Brady-Kalnay SM
    Mol. Cell. Neurosci., 2010-03-01;44(1):78-93.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: Bioassay
  9. Fibrogenic fibroblasts increase intercellular adhesion strength by reinforcing individual OB-cadherin bonds.
    Authors: Pittet P, Lee K, Kulik AJ, Meister JJ, Hinz B
    J. Cell. Sci., 2008-02-26;121(0):877-86.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Binding Assay
  10. Age- and dose-related effects on MSC engraftment levels and anatomical distribution in the central nervous systems of nonhuman primates: identification of novel MSC subpopulations that respond to guidance cues in brain.
    Authors: Isakova IA, Baker K, Dutreil M, Dufour J, Gaupp D, Phinney DG
    Stem Cells, 2007-10-11;25(12):3261-70.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Bioassay
  11. E-cadherin promotes retinal ganglion cell neurite outgrowth in a protein tyrosine phosphatase-mu-dependent manner.
    Authors: Oblander SA, Ensslen-Craig SE, Longo FM, Brady-Kalnay SM
    Mol. Cell. Neurosci., 2007-02-02;34(3):481-92.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: Bioassay

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