OX40 Ligand (OX40L), also known as gp34, is a type II transmembrane glycoprotein belonging to the TNF superfamily. Human OX40L cDNA encodes a 183 amino acids (aa) polypeptide with an amino-terminal cytoplasmic domain (aa 1¬23) and a carboxy¬terminal extracellular domain (aa 51-183). It shares 46% aa sequence identity with the mouse counterpart. OX40L is expressed on the surface of activated B cells, T cells, dendritic cells and endothelial cells. Similarly to other TNF superfamily members, membrane-bound OX40 Ligand exists as a homotrimer. OX40L binds to OX40 (CD134), a member of the TNF receptor superfamily that is expressed predominantly on activated CD4+ T cells. OX40 Ligand is one of the co¬stimulatory molecules in the immune system that includes B7, CD40 Ligand, CD30 Ligand, CD27 Ligand and 4¬1BB Ligand. Because OX40 appears as a late activation-induced T cell surface antigen, it has been speculated that the major function of OX40-OX40L interaction is to transmit a late co-stimulatory signal to promote the survival and proliferation of activated CD4+ T cells and prolong the immune response. Engagement of OX40 on activated T cells in situ in tumors has been shown to augment immune responses and subsequent tumor regression.
Recombinant Human OX40 Ligand/TNFSF4 Protein, CF
R&D Systems | Catalog # 1054-OX
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Key Product Details
- R&D Systems NS0-derived Recombinant Human OX40 Ligand/TNFSF4 Protein (1054-OX)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
NS0
Accession Number
Applications
Binding Activity, Bioactivity
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Product Specifications
Source
Mouse myeloma cell line, NS0-derived human OX40 Ligand/TNFSF4 protein
| MHHHHHHHHHH | GGGSGGGSGGGS | IEGR | Human OX40 Ligand (Gln51-Leu183) Accession # Q6FGS4 |
| N-terminus | C-terminus | ||
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
18 kDa
SDS-PAGE
28-34 kDa, reducing conditions
Activity
Measured by its ability to induce IL-8 secretion in HT1080 human fibrosarcoma cells transfected with human OX40/TNFRSF4. Muller, N. et al. (2008) FEBS J. 275:2296.
The ED50 for this effect is typically 1-5 ng/mL.
Measured by its binding ability in a functional ELISA.
When Recombinant Human OX40/TNFRSF4 Fc Chimera (Catalog # 3388-OX) is immobilized at 10 ng/mL, the concentration of Recombinant Human OX40 Ligand/TNFSF4 that produces 50% optimal binding response is found to be approximately 0.5‑3 ng/mL.
The ED50 for this effect is typically 1-5 ng/mL.
Measured by its binding ability in a functional ELISA.
When Recombinant Human OX40/TNFRSF4 Fc Chimera (Catalog # 3388-OX) is immobilized at 10 ng/mL, the concentration of Recombinant Human OX40 Ligand/TNFSF4 that produces 50% optimal binding response is found to be approximately 0.5‑3 ng/mL.
Reviewed Applications
Read 5 reviews rated 5 using 1054-OX in the following applications:
Scientific Data Images for Recombinant Human OX40 Ligand/TNFSF4 Protein, CF
Recombinant Human OX40 Ligand/TNFSF4 Protein Bioactivity
Recombinant Human OX40 Ligand/TNFSF4 (Catalog # 1054-OX) induces IL-8 secretion in the HT1080 human fibrsarcoma cell line. The ED50 for this effect is 1-5 ng/mL.
Recombinant Human OX40 Ligand/TNFSF4 Protein SDS-PAGE
1 μg/lane of Recombinant Human OX40 Ligand/TNFSF4 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing bands at 28-34 kDa.Formulation, Preparation, and Storage
1054-OX
| Formulation | Supplied as a 0.2 μm filtered solution in PBS. |
| Reconstitution |
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| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: OX40 Ligand/TNFSF4
References
- Godfrey, W.R. et al. (1994) J. Exp. Med. 180:757
- Baum, P.R. et al. (1994) EMBO J. 13:3992.
- AlShamkhani, A. et al. (1997) J. Biol. Chem. 272:5275.
- Kjaergaard, J. et al. (2000) Cancer Res. 60:5514.
Alternate Names
CD252, gp34, TNFSF4
Gene Symbol
TNFSF4
UniProt
Additional OX40 Ligand/TNFSF4 Products
Product Documents for Recombinant Human OX40 Ligand/TNFSF4 Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human OX40 Ligand/TNFSF4 Protein, CF
For research use only
Citations for Recombinant Human OX40 Ligand/TNFSF4 Protein, CF
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Customer Reviews for Recombinant Human OX40 Ligand/TNFSF4 Protein, CF (5)
5 out of 5
5 Customer Ratings
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 08/15/2025
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Application: In vivo studyVerified Customer | Posted 03/25/2019
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Application: Cell migration/motilityVerified Customer | Posted 10/24/2018
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Application: SDS-PAGE ControlVerified Customer | Posted 10/12/2018
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Associated Pathways
TNF Superfamily Pathway: Human Ligand-Receptor Interactions & their Associated Functions
