Recombinant Human OX40 Ligand/TNFSF4 Protein, CF

Catalog # Availability Size / Price Qty
1054-OX-025

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Recombinant Human OX40 Ligand/TNFSF4 Protein Bioactivity
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Citations (7)
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Recombinant Human OX40 Ligand/TNFSF4 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce IL-8 secretion in HT1080 human fibrosarcoma cells transfected with human OX40/TNFRSF4. Muller, N. et al. (2008) FEBS J. 275:2296. The ED50 for this effect is typically 1-5 ng/mL. Measured by its binding ability in a functional ELISA. When Recombinant Human OX40/TNFRSF4 Fc Chimera (Catalog # 3388-OX) is immobilized at 10 ng/mL, the concentration of Recombinant Human OX40 Ligand/TNFSF4 that produces 50% optimal binding response is found to be approximately 0.5‑3 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human OX40 Ligand/TNFSF4 protein
MHHHHHHHHHH GGGSGGGSGGGS IEGR Human OX40 Ligand
(Gln51-Leu183)
Accession # Q6FGS4
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
18 kDa
SDS-PAGE
28-34 kDa, reducing conditions

Product Datasheets

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1054-OX

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1054-OX

Formulation Supplied as a 0.2 μm filtered solution in PBS.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.

Scientific Data

Bioactivity Recombinant Human OX40 Ligand/TNFSF4 Protein Bioactivity View Larger

Recombinant Human OX40 Ligand/TNFSF4 (Catalog # 1054-OX) induces IL-8 secretion in the HT1080 human fibrsarcoma cell line. The ED50 for this effect is 1-5 ng/mL.

SDS-PAGE Recombinant Human OX40 Ligand/TNFSF4 Protein SDS-PAGE View Larger

1 μg/lane of Recombinant Human OX40 Ligand/TNFSF4 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing bands at 28-34 kDa.

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Background: OX40 Ligand/TNFSF4

OX40 Ligand (OX40L), also known as gp34, is a type II transmembrane glycoprotein belonging to the TNF superfamily. Human OX40L cDNA encodes a 183 amino acids (aa) polypeptide with an amino-terminal cytoplasmic domain (aa 1¬23) and a carboxy¬terminal extracellular domain (aa 51-183). It shares 46% aa sequence identity with the mouse counterpart. OX40L is expressed on the surface of activated B cells, T cells, dendritic cells and endothelial cells. Similarly to other TNF superfamily members, membrane-bound OX40 Ligand exists as a homotrimer. OX40L binds to OX40 (CD134), a member of the TNF receptor superfamily that is expressed predominantly on activated CD4+ T cells. OX40 Ligand is one of the co¬stimulatory molecules in the immune system that includes B7, CD40 Ligand, CD30 Ligand, CD27 Ligand and 4¬1BB Ligand. Because OX40 appears as a late activation-induced T cell surface antigen, it has been speculated that the major function of OX40-OX40L interaction is to transmit a late co-stimulatory signal to promote the survival and proliferation of activated CD4+ T cells and prolong the immune response. Engagement of OX40 on activated T cells in situ in tumors has been shown to augment immune responses and subsequent tumor regression.

References
  1. Godfrey, W.R. et al. (1994) J. Exp. Med. 180:757
  2. Baum, P.R. et al. (1994) EMBO J. 13:3992.
  3. Al­Shamkhani, A. et al. (1997) J. Biol. Chem. 272:5275.
  4. Kjaergaard, J. et al. (2000) Cancer Res. 60:5514.
Entrez Gene IDs
7292 (Human); 22164 (Mouse); 89814 (Rat)
Alternate Names
CD134 ligand; CD134L; CD252 antigen; CD252; Glycoprotein Gp34; gp34; OX40 antigen ligand; OX40 Ligand; OX40L; OX-40L; OX4OL; TAX transcriptionally-activated glycoprotein 1; tax-transcriptionally activated glycoprotein 1 (34kD); tax-transcriptionally activated glycoprotein 1, 34kD; TNFSF4; tumor necrosis factor (ligand) superfamily, member 4; tumor necrosis factor ligand superfamily member 4; TXGP1

Citations for Recombinant Human OX40 Ligand/TNFSF4 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. A multivariate modeling framework to quantify immune checkpoint context-dependent stimulation on T cells
    Authors: L Karpf, C Trichot, L Faucheux, I Legbre, M Grandclaud, C Lahoute, H Mattoo, B Pasquier, V Soumelis
    Cell Discovery, 2022-01-04;8(1):1.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Restoration of T Cell function in multi-drug resistant bacterial sepsis after interleukin-7, anti-PD-L1, and OX-40 administration
    Authors: LK Thampy, KE Remy, AH Walton, Z Hong, K Liu, R Liu, V Yi, CD Burnham, RS Hotchkiss
    PLoS ONE, 2018-06-26;13(6):e0199497.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. An anti-TL1A antibody for the treatment of asthma and inflammatory bowel disease
    Authors: AW Clarke, L Poulton, D Shim, D Mabon, D Butt, M Pollard, V Pande, J Husten, J Lyons, C Tian, AG Doyle
    MAbs, 2018-03-05;0(0):1-43.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance (SPR
  4. Production and characterization of a novel site-specific-modifiable anti-OX40-receptor single-chain variable fragment for targeted drug delivery
    Authors: A Tanabe, K Nakano, M Nakakido, S Nagatoishi, Y Tanaka, K Tsumoto, K Uchimaru, T Watanabe
    Biochem. Biophys. Res. Commun., 2018-01-09;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Murine insulin growth factor-like (IGFL) and human IGFL1 proteins are induced in inflammatory skin conditions and bind to a novel tumor necrosis factor receptor family member, IGFLR1.
    Authors: Lobito AA, Ramani SR, Tom I, Bazan JF, Luis E, Fairbrother WJ, Ouyang W, Gonzalez LC
    J. Biol. Chem., 2011-03-31;286(21):18969-81.
    Species: Human
    Sample Types:
    Applications: Surface Plasmon Resonance
  6. Mycobacterium bovis Bacillus Calmette-Guerin suppresses inflammatory Th2 responses by inducing functional alteration of TSLP-activated dendritic cells.
    Authors: Yokoi T, Amakawa R, Tanijiri T, Sugimoto H, Torii Y, Amuro H, Son Y, Tajima K, Liu YJ, Ito T, Fukuhara S
    Int. Immunol., 2008-08-13;20(10):1321-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Mapping the domains of CD134 as a functional receptor for feline immunodeficiency virus.
    Authors: Willett BJ, McMonagle EL, Bonci F, Pistello M, Hosie MJ
    J. Virol., 2006-08-01;80(15):7744-7.
    Species: Feline
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Human OX40 Ligand/TNFSF4 Protein, CF

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Recombinant Human OX40 Ligand/TNFSF4 Protein, CF
By Jenna Nguyen on 03/25/2019
Application: In vivo study

Recombinant Human OX40 Ligand/TNFSF4 Protein, CF
By Jenna Nguyen on 10/24/2018
Application: Cell migration/motility

Recombinant Human OX40 Ligand/TNFSF4 Protein, CF
By Jenna Nguyen on 10/12/2018
Application: SDS-PAGE Control

Recombinant Human OX40 Ligand/TNFSF4 Protein, CF
By Jenna Nguyen on 07/11/2018
Application: Cell Adhesion