Home / Pappalysin-1/PAPP-A / Recombinant Human Pappalysin-1/PAPP-A (aa 82-1214), CF

Recombinant Human Pappalysin-1/PAPP-A (aa 82-1214), CF

Catalog #: 2487-ZN
2 Citations Datasheet / COA / SDS
Newer Version Available: 2487-ZNF
NEW

Discontinued Product

2487-ZN has been discontinued and is replaced by 2487-ZNF.

R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Citations (2)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Pappalysin-1/PAPP-A (aa 82-1214), CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to cleave IGFBP-5. Cleavage of Recombinant Human IGFBP‑5 (Catalog # 875-B5) by Recombinant Human Pappalysin‑1/PAPP‑A  is >50%, as measured under the described conditions.
The IGFBP-5 cleaving activity of rhPAPP-A can be inhibited by 10 mM 1,10-phenanthroline.
Source
Mouse myeloma cell line, NS0-derived human Pappalysin-1/PAPP-A protein
Glu82-Asp1214 and Arg84-Asp1214, both with a C-terminal 10-His tag
Accession #
Q13219
N-terminal Sequence
Analysis
Glu82 & Arg84
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
128 kDa
SDS-PAGE
115 kDa and 150 kDa, reducing conditions

Product Datasheets

You must select a language.

x

2487-ZN

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2487-ZN

Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and CaCl2.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Assay Procedure

Materials
  • Assay Buffer: 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, pH 7.5 (TCN)
  • Recombinant Human Pappalysin-1/PAPP-A (rhPappalysin-1) (Catalog # 2487-ZN)
  • Recombinant Human IGFBP‑5 (rhIGFBP-5) (Catalog # 875-B5)
  • Reducing SDS-PAGE gel buffer
  • SDS-PAGE and/or Western blot
  1. Dilute rhPappalysin-1 to 92.8 µg/mL in Assay Buffer.
  2. Dilute rhIGFBP-5 to 200 µg/mL in Assay Buffer.
  3. Combine equal volumes of 92.8 µg/mL rhPappalysin-1 and 200 µg/mL rhIGFBP-5. Include two controls containing Assay Buffer in place of rhPappalysin-1.
  4. Incubate reaction vials and one control at 37 °C for 30 minutes. Keep the other control at -20 °C during the 30 minute incubation period.
  5. Combine equal volumes of rhPappalysin-1/rhIGFBP-5 reaction mixuture and reducing SDS-PAGE gel buffer.
  6. Analyze the cleavage by SDS-PAGE (load 20 μL/lane) followed by protein staining and/or Western blot.
Per Lane:
  • rhPappalysin-1: 0.464 μg
  • rhIGFBP-5: 1 µg
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Pappalysin-1/PAPP-A

Pappalysins belong to a fifth family of metzincins that consists of ADAMs/ADAMTSs, MMPs, astacins and serrylysins (1). PAPP-A is an important pregnancy protein and increases in plasma by a factor of about 150 during pregnancy as compared to the nonpregnant state. PAPP-A is also a major marker of Down syndrome in the first trimester of pregnancy because maternal serum levels ofPAPP-A are significantly reduced when a fetus affected by Down syndrome is present (2). PAPP-A cleaves Insulin-like Growth Factor-Binding Protein-4 and -5 (IGFBP-4 and -5) at a single site, resulting in the release of bioactive IGF (3). Lack of IGFBP-4 cleavage in embryonic fibroblasts derived from PAPP-A knockout mice indicates that PAPP-A functions as a physiological IGFBP-4 protease (4). Three Lin12-Notch repeats (LNR) in the PAPP-A protein bind Ca2+ and are required for the cleavage of IGFBP-4, not IGFBP-5, by PAPP-A (5). The C-terminal LNR (residues 1476 to 1503) is not present in rhPAPP-A (residues 82 to 1214), which starts at the N-terminus of the mature chain and ends before the five Sushi (SCR) modules. As an active protease, rhPAPP-A cleaves IGFBP-5, which can be inhibited by 1,10-phenanthroline.

References
  1. Boldt, H.B. et al. (2001) Biochem. J. 358:359.
  2. Fialova L. and I.M. Malbohan (2002) Bratisl. Lek. Listy 103:194.
  3. Laursen, L.S. et al. (2001) FEBS Lett. 504:36.
  4. Conover, C.A. et al. (2004) Development 131:1187.
  5. Boldt, H.B. et al. (2004) J. Biol. Chem. 279:38525.
Long Name
Pregnancy-associated Plasma Protein 1
Entrez Gene IDs
5069 (Human); 18491 (Mouse)
Alternate Names
ASBABP2; DIPLA1; EC 3.4.24.79; IGFBP-4ase; IGFBP-4aseaspecific BCL2 ARE-binding protein 2; IGF-dependent IGFBP-4 protease; insulin-like growth factor-dependent IGF binding protein-4 protease; Insulin-like growth factor-dependent IGF-binding protein 4 protease; PAPA; PAPPA; PAPPA1; PAPP-A1; PAPP-Adifferentially placenta 1 expressed protein; Pappalysin1; Pappalysin-1; pregnacy-associated plasma protein A; Pregnancy-associated plasma protein A; pregnancy-associated plasma protein A, pappalysin 1

Citations for Recombinant Human Pappalysin-1/PAPP-A (aa 82-1214), CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Inhibition of longevity regulator PAPP-A modulates tissue homeostasis via restraint of mesenchymal stromal cells
    Authors: M Mohrin, J Liu, J Zavala-Sol, S Bhargava, J Maxwell Tr, A Brito, D Hu, D Brooks, G Koukos, L Alabdulaal, JS Paw, K Hake, G Kolumam, ML Bouxsein, R Baron, Y Kutskova, A Freund
    Aging Cell, 2021-02-09;0(0):e13313.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Increased levels of pregnancy-associated plasma protein-A2 in the serum of pre-eclamptic patients.
    Authors: Nishizawa H, Pryor-Koishi K, Suzuki M
    Mol. Hum. Reprod., 2008-09-18;14(10):595-602.
    Species: Human
    Sample Types: Buffer
    Applications: Antibody Absorption

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human Pappalysin-1/PAPP-A (aa 82-1214), CF

There are currently no reviews for this product. Be the first to review Recombinant Human Pappalysin-1/PAPP-A (aa 82-1214), CF and earn rewards!

Have you used Recombinant Human Pappalysin-1/PAPP-A (aa 82-1214), CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review