Recombinant Human PD-L1/B7-H1 His-tag Avi-tag Protein, CF

R&D Systems | Catalog # AVI9049

Biotinylated
R&D Systems
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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human PD-L1/B7-H1 His-tag Avi-tag Protein (AVI9049)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

Q9NZQ7

Structure / Form

Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity
View all PD-L1/B7-H1 Proteins and Enzymes »

Why choose R&D Systems Avi-tag PD-L1 Protein?

  • Guaranteed Bioactivity and High Purity: Bioactivity tested by functional ELISA and purity determined by SDS-PAGE to be greater than 95%.
  • Lot-to-Lot Consistency: Stringent QC testing performed on each lot to ensure consistent activity and purity.
  • Bulk Quantities Available: Bulk up and save with large mass quantities to meet your research needs. Supply agreements available, partner with us. Please contact us.
  • Most Respected, Most Cited Brand in Proteins: With over 35 years of providing the best recombinant proteins to the scientific community, R&D Systems continues to lead the industry in quality, activity, and purity.

Find the Fc Chimera Avi-tag PD-L1 protein here Fc Chimera Avi-tag PD-L1 Protein

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human PD-L1/B7-H1 protein
Human B7-H1
(Phe19-Thr239)
Accession # Q9NZQ7		 HHHHHH Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe19

Predicted Molecular Mass

28 kDa

SDS-PAGE

35-41 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human PD-1 Fc Chimera (Catalog # 1086-PD) is immobilized at 2 µg/mL (100 µL/well), Biotinylated Recombinant Human PD-L1 His-tag Avi-tag protein binds with an ED50 of 2-12 μg/mL.

Scientific Data Images for Recombinant Human PD-L1/B7-H1 His-tag Avi-tag Protein, CF

Recombinant Human PD-L1/B7-H1 His-tag Avi-tag Protein Binding Activity

Recombinant Human PD-L1/B7-H1 His-tag Avi-tag Protein Binding Activity

When Recombinant Human PD-1 Fc Chimera (Catalog # 1086-PD) is immobilized at 2 µg/mL, Biotinylated Recombinant Human PD-L1 His-tag Avi-tag protein binds with an ED50 of 2-12 µg/mL.
Recombinant Human PD-L1/B7-H1 His-tag Avi-tag Protein Flow Cytometry

Recombinant Human PD-L1/B7-H1 His-tag Avi-tag Protein Flow Cytometry

In a functional flow cytometry test, (A) Recombinant Human PD-L1/B7-H1 His-tag Avi-tag Protein (Catalog # AVI9049) binds to HEK293 human embryonic kidney cell line transfected with recombinant human PD-1 and EGFP. Ligand binding was detected by staining cells with APC-conjugated Streptavidin (Catalog # F0050), which does not stain the cells in the absence of recombinant protein (B).
Recombinant Human PD-L1/B7-H1 His-tag Avi-tag Protein SDS-PAGE

Recombinant Human PD-L1/B7-H1 His-tag Avi-tag Protein SDS-PAGE

2 μg/lane of Biotinylated Recombinant Human PD-L1/B7-H1 His Tag Avi Tag was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 35-41 kDa.
Surface plasmon resonance sensorgram of Avi-tag biotinylated human PD-L1/B7-H1 protein binding to human PD-1

Binding of Human PD-1 to PD-L1/B7-H1 His Avi-tag Protein by surface plasmon resonance (SPR).

Avi-tag Biotinylated Recombinant Human PD-L1/B7-H1 His protein (Catalog # AVI9049) was immobilized on a Biacore Sensor Chip CM5 via the Avi-tag biotin, and binding to Recombinant Human PD-1 His protein (8986-PD) was measured at a concentration range between 3.2 nM and 13.2 µM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=1.122 µM.

Formulation, Preparation, and Storage

AVI9049
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PD-L1/B7-H1

B7-H1, also known as PD-L1 and CD274, is an approximately 65-kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules (1). Mature human B7-H1 consists of a 220 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 31 aa cytoplasmic domain (2). Within the ECD, human B7-H1 shares 73% and 74% aa sequence identity with mouse and rat B7-H1, respectively. Alternative splicing generates additional isoforms that either lack the first Ig-like domain or are truncated within the second Ig-like domain (3). B7-H1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells (4-7), keratinocytes (8, 9), endothelial and intestinal epithelial cells (8, 10), as well as a variety of carcinomas and melanoma (11, 12). B7-H1 binds to T cell B7-1/CD80 and PD-1 (7, 8, 12-15). It suppresses T cell activation and proliferation (5, 8, 14, 16) and induces the apoptosis of activated T cells (11). It plays a role in the development of immune tolerance by promoting T cell anergy (7, 14) and enhancing regulatory T cell development (16). B7-H1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells (5, 10) and inhibits the development of Th17 cells (16). In cancer, B7-H1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells (6, 9, 12, 15).

References

  1. Ceeraz, S. et al. (2013) Trends Immunol. 34:556.
  2. Dong, H. et al. (1999) Nat. Med. 5:1365.
  3. Frigola, X. et al. (2011) Clin. Cancer Res. 17:1915.
  4. Tamura, H. et al. (2001) Blood 97:1809.
  5. Chen, L. et al. (2007) J. Immunol. 178:6634.
  6. Kuang, D.-M. et al. (2014) J. Clin. Invest. 124:4657.
  7. Tsushima, F. et al. (2007) Blood 110:180.
  8. Mazanet, M.M. and C.C.W. Hughes (2002) J. Immunol. 169:3581.
  9. Cao, Y. et al. (2010) Cancer Res. 71:1235.
  10. Scandiuzzi, L. et al. (2014) Cell Rep. 6:625.
  11. Dong, H. et al. (2002) Nat. Med. 8:793.
  12. Azuma, T. et al. (2008) Blood 111:3635.
  13. Butte, M.J. et al. (2008) Mol. Immunol. 45:3567.
  14. Park, J.-J. et al. (2010) Blood 116:1291.
  15. Ritprajak, P. et al. (2010) J. Immunol. 184:4918.
  16. Herold, M. et al. (2015) J. Immunol. 195:3584.

Long Name

Programmed Death Ligand 1

Alternate Names

B7-H1, B7H1, CD274, PDCD1L1, PDCD1LG1, PDL1

Entrez Gene IDs

29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)

Gene Symbol

CD274

UniProt

Q9NZQ7

Additional PD-L1/B7-H1 Products

Product Documents for Recombinant Human PD-L1/B7-H1 His-tag Avi-tag Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human PD-L1/B7-H1 His-tag Avi-tag Protein, CF

For research use only

Citations for Recombinant Human PD-L1/B7-H1 His-tag Avi-tag Protein, CF

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