Recombinant Human Periostin/OSF-2 GMP Protein, CF

Name: Recombinant Human Periostin/OSF-2 GMP Protein, CF
Brand: R&D Systems
SKU: 3548-GMP

Catalog #: 3548-GMP Datasheet / COA / SDS

Discontinued Product

3548-GMP has been discontinued.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Periostin/OSF-2 GMP Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. Immobilized Recombinant Human Periostin/OSF‑2 at 10 µg/mL (100 µL/well) induces >50% cell adhesion.

Source

Mouse myeloma cell line, NS0-derived human Periostin/OSF-2 protein
Asn22-Gln836
Manufactured and tested under cGMP guidelines.

Accession #

Q15063

N-terminal Sequence
Analysis

Asn22-Asn-His-Tyr-Asp-Lys-Ile-Leu-Ala-His

Predicted Molecular Mass

91.7 kDa

SDS-PAGE

85-90 kDa

Product Datasheets

3548-GMP

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3548-GMP

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Immobilized Recombinant Human Periostin/ OSF‑2 (Catalog # 3548-GMP) at 10 µg/mL (100 µL/well) induces >50% cell adhesion.

Reconstitution Calculator

Background: Periostin/OSF-2

Periostin, also known as OSF-2, is a secreted matricellular protein with functions in extracellular matrix formation, cell migration, and inflammation (1). It is secreted as a 90 kDa monomer that can aggregate into >170 kDa higher-order multimers (2). Periostin contains an N-terminal EMI domain followed by four tandem FAS1 domains (3). Mature human Periostin shares 91% aa sequence identity with mouse and rat Periostin. Alternative splicing generates additional isoforms with various deletions in the C-terminal region following the FAS domains. Periostin is expressed by mesenchymal cells such as vascular smooth muscle cells, fibroblasts, osteoblasts, and odontoblasts in developing teeth (4-7). It is up-regulated in many carcinomas (2, 8). Periostin binds to Integrins alpha _v beta ₃ and alpha _v beta ₅ (2, 9), leading to enhanced cell adhesion and cell migration (2, 5, 6). It enhances Fibronectin and Collagen I production and promotes collagen fibrillogenesis (10, 11). It also induces epithelial-mesenchymal transition, tumor growth, invasion, and metastasis (9). Periostin induces the expression of VEGF R2 on endothelial cells and VEGF-C in tumor cells, and it can induce tumor lymphangiogenesis (8, 12). Periostin plays an important role in heart valve development and tissue healing after myocardial infarction (5, 13, 14). In asthma, it is up-regulated in bronchial epithelium and plays both destructive and protective roles by inducing eosinophil infiltration and inhibiting goblet cell metaplasia and mucus production, respectively (15, 16).

References

Liu, A.Y. et al. (2014) Matrix Biol. 37:150.
Gillan, L. et al. (2002) Cancer Res. 62:5358.
Takeshita, S. et al. (1993) Biochem. J. 294:271.
Kruzynska-Frejtag, A. et al. (2004) Dev. Dyn. 229:857.
Lindner, V. et al. (2005) Arterioscler. Thromb. Vasc. Biol. 25:77.
Horiuchi, K. et al. (1999) J. Bone Miner. Res. 14:1239.
Li, G. et al. (2006) Atherosclerosis 188:292.
Shao, R. et al. (2004) Mol. Cell. Biol. 24:3992.
Yan, W. and R. Shao (2006) J. Biol. Chem. 281:19700.
Erkan, M. et al. (2007) Gastroenterology 132:1447.
Norris, R.A. et al. (2007) J. Cell. Biochem. 101:695.
Kudo, Y. et al. (2012) PLoS One 7:e44488.
Snider, P. et al. (2008) Circ. Res. 102:752.
Kuhn, B. et al. (2007) Nat. Med. 13:962.
Blanchard, C. et al. (2008) Mucosal Immunol. 1:289.
Sehra, S. et al. (2011) J. Immunol. 186:4959.

Long Name

Osteoblast Specific Factor 2

Entrez Gene IDs

10631 (Human); 50706 (Mouse); 361945 (Rat)

Alternate Names

Fasciclin I-like; MGC119510; MGC119511; OSF2; OSF-2; OSF-2osteoblast specific factor 2 (fasciclin I-like); OSF2periodontal ligament-specific periostin; Osteoblast-specific factor 2; PDLPOSTN; periostin isoform thy2; periostin isoform thy4; periostin isoform thy6; periostin isoform thy8; Periostin; periostin, osteoblast specific factor; PNRP11-412K4.1; POSTN; TRIF52

Manufacturing Specifications

GMP Proteins
R&D Systems, a Bio-Techne Brand's GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.

R&D Systems' quality focus includes:

Manufactured and tested under an ISO 9001:2015 and ISO 13485:2016 certified quality system
Documented processes and QA control of documentation and process changes
Personnel training programs
Raw material testing and vendor qualification/monitoring
Fully validated equipment, processes and test methods
Equipment calibration schedules using a computerized calibration program
Facility maintenance, safety programs and pest control
Material review process for variances
Monitoring of stability over product shelf-life

R&D Systems strives to provide our customers with the analytical characteristics of each product so that customers may determine whether our products are appropriate for their research. The Certificate of Analysis provided contains the following lot specific information:

N-terminal amino acid analysis, SDS-PAGE analysis, and endotoxin level (as determined by LAL assay) performed on each bulk QC lot, not on individual bottlings of each QC lot
Post-bottling lot-specific bioassay results (compliance with an established range) and results of microbial bioburden testing (using broth culture, Sabourand's dextrose and blood agar plates with results reported at 3 days and at 7 days)
Mycoplasma testing by ribosomal RNA hybridization assay

Additional testing and documentation requested by the customer can be arranged at an additional cost. Testing may include, but is not limited to, USP <61> bioburden testing, positive identity testing, testing for adventitious agents and testing for residual host cell content.

Production records and facilities are available for examination by appropriate personnel on-site at R&D Systems in Minneapolis, Minnesota USA.

R&D Systems sells GMP grade products for preclinical or clinical ex vivo use. They are not for in vivo use. Please read the following End User Terms prior to using this product.