Recombinant Human Prolactin Protein, CF 682-PL-050: R&D Systems

Recombinant Human Prolactin Protein, CF

(2 citations)   
  • Purity
    >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using Nb2‑11 rat lymphoma cells. Gout, P.W. et al. (1980) Cancer Res. 40:2433. The ED50 for this effect is 0.03‑0.1 ng/mL.
  • Source
    E. coli-derived Leu29-Cys227, with an N-terminal Met
  • Accession #
  • N-terminal Sequence
    Analysis
    Met
  • Predicted Molecular Mass
    24 kDa
682-PL
 
Formulation Lyophilized from a 0.2 μm filtered solution in Phosphate and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl containing 1 mg/mL bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Prolactin

Prolactin (gene name PRL) is a secreted neuroendocrine pituitary hormone that acts primarily on the mammary gland to promote lactation, but has pleiotropic effects in both males and females (1-6). Prolactin is predominantly found as 199 amino acid, 25 kDa glycosylated and 23 kDa non-glycosylated monomers (6). Human prolactin shares only 60% and 63% amino acid sequence identity with mouse and rat prolactin, respectively, although rat prolactin can activate the human prolactin receptor (3). Post-translational modifications such as polymerization, complex formation with IgG (in humans), glycosylation, and proteolytic cleavage can alter the activities of prolactin (6-8). Non-glycosylated prolactin is produced by the pituitary and packaged in storage granules before secretion, while glycosylated prolactin is reported to be constitutively secreted, have lower biological potency, and be removed from the circulation more quickly (3, 6, 7). Cleavage by matrix metalloproteinases or Cathepsin D can produce N-terminal 16 kDa antiangiogenic fragments also called vasoinhibins (9, 10). Thrombin can produce C-terminal 16 kDa fragments that are not antiangiogenic (3). Prolactin is synthesized mainly by the anterior pituitary in all mammals, where secretion is under tonic inhibition by hypothalamic dopamine (2, 3). In humans, prolactin is also produced peripherally (2-5). Prolactin expression is low during early human pregnancy, but increases in late pregnancy (2, 3). The prolactin receptor (gene name PRLR) is a transmembrane type I glycoprotein that belongs to the cytokine hematopoietic receptor family. Expression of the prolactin receptor is widespread (2-5). Each prolactin molecule is thought to bind two receptor molecules (11). In addition to its lactogenic activity, peripherally produced prolactin plays roles in breast and prostate cancer development, regulation of reproductive function, and immunoregulation (5, 6).

  • References:
    1. Cooke, N.E. et al. (1981) J. Biol. Chem. 256:4007.
    2. Grattan, D.R. and I.C. Kokay (2008) J. Neuroendocrinol. 20:752.
    3. Ben-Jonathan, N. et al. (2008) Endocr. Rev. 29:1.
    4. Bernichtein, S. et al. (2010) J. Endocrinol. 206:1.
    5. Goffin, V. et al. (2011) Nat. Rev. Urology 8:597.
    6. Price, A.E. et al. (1995) Endoc. 136:4827.
    7. Hoffmann, T. et al. (1993) J. Endoc. Invest. 16:807.
    8. Cole, E. et al. (1991) Endoc. 129:2639.
    9. Piwnica, D. et al. (2006) Mol. Endocrinol. 20:3263.
    10. Macotela, Y. et al. (2006) J. Cell Sci. 119:1790.
    11. Broutin, I. et al. (2010) J. Biol. Chem. 285:8422.
  • Entrez Gene IDs:
    5617 (Human); 19109 (Mouse)
  • Alternate Names:
    PRL; Prolactin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Species
Applications
Sample Type
  1. Immunoregulation of autocrine prolactin: suppressing the expression of costimulatory molecules and cytokines in T lymphocytes by prolactin receptor knockdown.
    Authors: Xu D, Lin L, Lin X
    Cell. Immunol., 2010;263(1):71-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Prolactin and heregulin override DNA damage-induced growth arrest and promote phosphatidylinositol-3 kinase-dependent proliferation in breast cancer cells.
    Authors: Chakravarti P, Henry MK, Quelle FW
    Int. J. Oncol., 2005;26(2):509-14.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay

Have you used Recombinant Human Prolactin Protein, CF?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

    Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Feedback

Customer Information