Recombinant Human Protein S/PROS1, CF

Catalog # Availability Size / Price Qty
9489-PS-100
Recombinant Human Protein S/PROS1, CF Data
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Recombinant Human Protein S/PROS1, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Tyro3/Dtk Fc Chimera (Catalog # 859-DK) is immobilized at 1 μg/mL, 100 μL/well, Recombinant Human Protein S/PROS1 binds with an ED50 of 0.2-1.2 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human Protein S/PROS1 protein
Ala42-Trp670, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Analysis
Ala42
Predicted Molecular Mass
71 kDa
SDS-PAGE
66-93 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9489-PS

Formulation Lyophilized from a 0.2 μm filtered solution in MES, NaCl and CaCl2.
Reconstitution Reconstitute at 1 mg/mL in water.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity View Larger

When Recombinant Human Tyro3/Dtk Fc Chimera (Catalog # 859-DK) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human Protein S/PROS1 (Catalog # 9489-PS) binds with an ED50of 0.2-1.2 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Protein S/PROS1

Anticoagulant Protein S (PROS1) is a 71 kDa plasma vitamin K-dependent glycoprotein characterized by the presence of the post-translational modification of specific glutamic acid residues to gamma-carboxyglutamic acid (Gla) in the N-terminal region. In addition to the N-terminal Gla domain, mature PROS1 contains a thrombin‑sensitive thumb loop, four tandem EGF-like domains and a C-terminal sex hormone-binding globulin (SHBG) domain composed of two Laminin G (LG) domains (1). Human PROS1 shares 80% amino acid sequence identity with mouse PROS1. Hundreds of mutations have been reported throughout all the domains of the protein, typically resulting in PROS1 deficiency and an increase in the risk of thrombophilia (2, 3). PROS1 is expressed in many cell types supporting its reported involvement in multiple biological processes that include coagulation, apoptosis, cancer development and progression, and the innate immune response (4-9). PROS1 exists in plasma both in complex with C4b-binding protein (C4BP) (60%) and in a free form (40%). Both forms of PROS1 have anti-coagulant activity either directly through inhibition of Factor X systems and prothrombinase while in complex with C4BP (5, 10) or in its well-established role as a cofactor of activated protein C (APC) inactivation of procoagulants FVa and FVIIIa (4). The free form of PROS1 is also a ligand for a subfamily of receptor tyrosine kinases known as TAMs, which is composed of TYRO3, AXL, and MERTK (11). PROS1 binds these tyrosine kinase receptors through its LG domains to activate downstream signaling pathways involved in tumor development and progression (7, 8, 11, 12).

References
  1. Dahlback, B. et al. (1986) Proc. Natl. Acad. Sci. 83:4199.
  2. Garcia de Frutos, P. et al. (2007) J. Thromb. Haemost. 98:543.
  3. Heeb, M. J. (2008) Expert Rev. Hematol. 1:9.
  4. Walker, F.J. (1984 Sem. Thromb. Hemosta. 10:131.
  5. Heeb, M. J. et al. (2004) J. Thromb. Haemost. 2:1766.
  6. Che Mat, M. F. (2016) Int. J. Oncol. 49:2359.
  7. Abboud-Jarrous, G. et al. (2017) Oncotarget. 8:13986.
  8. Qin, J. et al. (2016) Sci. Rep. 6:26662.
  9. Davra, V. et al. (2016) Cancers (Basel). 8:E107.
  10. Hackeng, T. M. et al. (1994) J. Biol. Chem. 269:21051.
  11. Tsou, W.I. et al. (2014) J. Biol. Chem. 289:25750.
  12. Linger, R.M.A. et al. (2008) Adv. Cancer Res. 100:35.
Entrez Gene IDs
5627 (Human); 19128 (Mouse); 81750 (Rat)
Alternate Names
EC 3.4.21; PROS; PROS1; protein S (alpha); Protein S; protein Sa; PS21; PS22; PS23; PS24; PS25; PSA; vitamin K-dependent plasma protein S; vitamin K-dependent protein S

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Recombinant Human Protein S/PROS1, CF
By Anonymous on 10/11/2018
Application: In vitro bioactivity in cell culture