Recombinant Human PSP94/MSMB Protein, CF

Catalog # Availability Size / Price Qty
3780-PS-050
Product Details
Citations (1)
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Recombinant Human PSP94/MSMB Protein, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized rhPSP94 at 1 µg/mL (100 µL/well) can bind rhCRISP-3 with a linear range of 0.03‑10 µg/mL.
Source
E. coli-derived human PSP94/MSMB protein
Ser21-Ile114, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ser21
Predicted Molecular Mass
11.7 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3780-PS

Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: PSP94/MSMB

PSP94 (prostate secretory protein of 94 amino acids; also named  beta -MSP) is a secreted, non-glycosylated member of the beta -microseminoprotein family (1). The 94 amino acid (aa) mature PSP94 contains no classic motifs or domains, but does have ten Cys that are conserved across species (1, 2). It is expressed in mucoid secretions, but its function is unknown (2, 3). PSP94 is abundant in prostatic fluid, which is the exclusive source in rodents (4). Gastric and respiratory secretory epithelia are also significant sources in humans (2, 3). Human PSP94 circulates bound to a 71 kDa PSP binding protein, possibly disulfide-linked (5). The seminal fluid protein CRISP-3 can also bind PSP94 (6). PSP94 has been proposed as an alternative to PSA as a serum marker for prostate cancer. When total (bound plus free) PSP94 is considered, its secretion is found to be down-regulated in cancer cells, creating below normal circulating levels (7, 8). Its size is predicted at 11 kDa, but may appear to be 16 kDa due to anomalous migration (3). A 61 aa variant, formed by C-terminal proteolysis, is increased in prostate secretions from patients with benign prostatic hyperplasia (9). A prostate-specific alternate splice form shows a substitution of 41 aa for the C-terminal 78 aa (10). Mature human PSP94 shares 53%, 46%, 43% and 42% aa identity with porcine, rat, mouse and chicken PSP94, respectively. Most of the ten primate sequences available show less than 80% aa identity. PSP94 has been proposed as a species barrier protein due to its low evolutionary conservation and abundance in seminal fluid (11).

References
  1. Seidah, N.G. et al. (1984) FEBS Lett. 175:349.
  2. Ulvsback, M. et al. (1989) Biochem. Biophys. Res. Comm. 164:1310.
  3. Weiber, H. et al. (1990) Am. J. Pathol. 137:593.
  4. Thota, A. et al. (2003) J. Cell. Biochem. 88:999.
  5. Wu, D. et al. (1999) J. Cell. Biochem. 76:71.
  6. Udby, L. et al. (2005) Biochem. Biophys. Res. Comm. 333:555.
  7. Reeves, J.R. et al. (2005) Biochem. J. 385:105.
  8. Nam, R.K. et al. (2006) J. Urol. 175:1291.
  9. Xu, K. et al. (2003) Electrophoresis 24:1311.
  10. Xuan, J.W. et al. (1995) Oncogene 11:1041.
  11. Xuan, J.W. et al. (1999) DNA Cell Biol. 18:11.
Long Name
Prostate Secretory Protein of 94 Amino Acids/Microseminoprotein, beta
Entrez Gene IDs
4477 (Human); 17695 (Mouse); 29311 (Rat)
Alternate Names
IGBF; MSMB; MSPB; PIP; PN44; PRPS; PSP57; PSP94

Citation for Recombinant Human PSP94/MSMB Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. beta-Microseminoprotein endows post coital seminal plasma with potent candidacidal activity by a calcium- and pH-dependent mechanism.
    Authors: Edstrom Hagerwall AM, Rydengard V, Fernlund P, Morgelin M, Baumgarten M, Cole AM, Malmsten M, Kragelund BB, Sorensen OE
    PLoS Pathog., 2012;8(4):e1002625.
    Species: Human
    Sample Types: Seminal Plasma
    Applications: Bioassay

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