Recombinant Human S100A9 Protein, CF

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Recombinant Human S100A9 Protein Bioactivity
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Product Details
Citations (4)
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Recombinant Human S100A9 Protein, CF Summary

Product Specifications

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to induce CXCL1/KC secretion by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.8-4 μg/mL.
E. coli-derived human S100A9 protein
Accession #
N-terminal Sequence
Predicted Molecular Mass
13 kDa
12 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in Tris, TCEP and Trehalose.
Reconstitution Reconstitute at 400 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human S100A9 Protein Bioactivity View Larger

Recombinant Human S100A9 (Catalog # 9254-S9) induces CXCL1/KC secretion in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.8-4 μg/mL.

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Background: S100A9

S100A9, also known as MRP14 and Calgranulin B, is an approximately 14 kDa pro-inflammatory protein in the S100 family of calcium binding proteins (1, 2). It is
up-regulated in neutrophils and monocytes at sites of inflammation (e.g. psoriasis, rheumatoid arthritis, cardiac ischemia) and is present at elevated concentrations in rheumatoid arthritis synovial fluid (3-6). Human S100A9 contains two EF-hand calcium binding motifs and shares 57% and 62% amino acid sequence identity with mouse and rat S100A9, respectively (7). S100A9 is a noncovalent homodimer that can also noncovalently heterodimerize with S100A8; in the presence of calcium and zinc, the homodimer and heterodimers will form tetramers (8-10). Human S100A9 can be phosphorylated at Thr113 near the C-terminus, a residue which is not conserved in the mouse protein (11). Human S100A9 can also be modified by S-glutathionylation and oxidation (12). The S100A8/A9 heterodimer binds to fatty acids such as arachidonic acid, while neither monomer does (4). S100A9 and the heterodimer promote neutrophil infiltration and degranulation at sites of inflammation and inflammatory cytokine production by monocytes (5, 6, 9, 13). S100A9 also promotes cartilage matrix destruction in osteoarthritis (14) as well as RAGE-dependent cardiac fibrosis and remodeling (6). In the brain, the S100A9 homodimer promotes neuronal cytotoxicity, although this is reduced by its binding to amyloid-beta peptide (15).

  1. Averill, M.M. et al. (2012) Arterioscler. Thromb. Vasc. Biol. 32:223.
  2. Vogl, T. et al. (2012) Int. J. Mol. Sci. 13:2893.
  3. Edgeworth, J. et al. (1991) J. Biol. Chem. 266:7706.
  4. Siegenthaler, G. et al. (1997) J. Biol. Chem. 272:9371.
  5. Sunahori, K. et al. (2006) Arthritis Res. Ther. 8:R69.
  6. Volz, H.C. et al. (2012) Basic Res. Cardiol. 107:250.
  7. Odink, K. et al. (1987) Nature 330:80.
  8. Teigelkamp, S. et al. (1991) J. Biol. Chem. 266:13462.
  9. Ryckman, C. et al. (2003) J. Immunol. 170:3233.
  10. Vogl, T. et al. (2006) Biochim. Biophys. Acta 1763:1298.
  11. Edgeworth, J. et al. (1989) Nature 342:189.
  12. Lim, S.Y. et al. (2010) J. Biol. Chem. 285:14377.
  13. Simard, J.-C. et al. (2010) J. Leukoc. Biol. 87:905.
  14. Schelbergen, R.F.P. et al. (2012) Arthritis Rheum. 64:1477.
  15. Zhang, C. et al. (2012) PLoS ONE 7:e32953.
Long Name
S100 Calcium Binding Protein A9
Entrez Gene IDs
6280 (Human); 20202 (Mouse)
Alternate Names
CAGBMigration inhibitory factor-related protein 14; Calgranulin B; calgranulin-B; Calprotectin L1H subunit; CFAGMRP-14,60B8AG; CGLB; L1AG; LIAG; MAC387; MIF; MRP-14; MRP14Leukocyte L1 complex heavy chain; NIF; P14; protein S100-A9; S100 calcium binding protein A9 (calgranulin B); S100 calcium binding protein A9; S100 calcium-binding protein A9 (calgranulin B); S100 calcium-binding protein A9; S100A9

Citations for Recombinant Human S100A9 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Stratification of radiosensitive brain metastases based on an actionable S100A9/RAGE resistance mechanism
    Authors: C Monteiro, L Miarka, M Perea-Garc, N Priego, P García-Góm, L Álvaro-Esp, A de Pablos-, N Yebra, D Retana, P Baena, C Fustero-To, O Graña-Cast, K Troulé, E Caleiras, P Tezanos, P Muela, E Cintado, JL Trejo, JM Sepúlveda, P González-L, L Jiménez-Ro, LM Moreno, O Esteban, Á Pérez-Núñe, A Hernández-, J Mazarico G, I Ferrer, R Suárez, EM Garrido-Ma, L Paz-Ares, C Dalmasso, E Cohen-Jona, A Siegfried, A Hegarty, S Keelan, D Varešlija, LS Young, M Mohme, Y Goy, H Wikman, J Fernández-, G Blasco, L Alcázar, C Cabañuz, SI Grivenniko, A Ianus, N Shemesh, CC Faria, R Lee, P Lorigan, E Le Rhun, M Weller, R Soffietti, L Bertero, U Ricardi, J Bosch-Barr, E Sais, E Teixidor, A Hernández-, A Calvo, J Aristu, SM Martin, A Gonzalez, O Adler, N Erez, RENACER, M Valiente
    Nature Medicine, 2022;28(4):752-765.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. AIM/CD5L attenuates DAMPs in the injured brain and thereby ameliorates ischemic stroke
    Authors: N Maehara, K Taniguchi, A Okuno, H Ando, A Hirota, Z Li, CT Wang, S Arai, T Miyazaki
    Cell Reports, 2021;36(11):109693.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Differential Regulation of Circulating Soluble Receptor for Advanced Glycation End Products (sRAGEs) and Its Ligands S100A8/A9 Four Weeks Post an Exercise Intervention in a Cohort of Young Army Recruits
    Authors: IA Drosatos, JN Tsoporis, S Izhar, S Gupta, G Tsirebolos, E Sakadakis, AS Triantafyl, A Rigopoulos, D Rigopoulos, LS Rallidis, I Rizos, TG Parker
    Biomolecules, 2021;11(9):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. S100A9 promotes prostate cancer cell invasion by activating TLR4/NF-&kappaB/integrin &beta1/FAK signaling
    Authors: Z Lv, W Li, X Wei
    OncoTargets and Therapy, 2020;13(0):6443-6452.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay


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