Recombinant Human S5a/Angiocidin Biotin Protein, CF

(1 citations)   
  • Purity
    >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
  • Activity
    Recombinant Human S5a/Angiocidin Biotin is ideal for use as a control substrate for in vitro Ubiquitin conjugation. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human S5a/Angiocidin Biotin concentration of 1-5 μM.
  • Source
    E. coli-derived
  • Accession #
  • Predicted Molecular Mass
    43 kDa
SP-405
 
Formulation Supplied as a solution in HEPES, NaCl, DTT and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Background: S5a/Angiocidin
S5a/Angiocidin, also known as Anti-secretory Factor (ASF), is classified under the gene PSMD4, but is often referred to by a different name depending on the context in which it is described. S5a and ASF have identical 377 amino acid (aa) sequences, while Angiocidin is described as having an additional Gly255Glu256Arg257 sequence in its C-Terminus (1-3). The human protein shares 96% and 99% aa sequence identity with its mouse and rat orthologs, respectively. Structurally, it contains an N-terminal von Willebrand Factor type A domain and two C-terminal Ubiquitin-interacting motifs (UIM). It acts as a Ubiquitin-binding protein where it is most commonly referred to as S5a or in yeast as Rpn10. It is part of the 19S regulatory subunit of the 26S Proteasome where its UIM recognizes poly-ubiquitinated proteins destined for degradation (4). As a part of the proteasome complex, it may also recognize the Ubiquitin-like modifier FAT10 (5). Free cytoplasmic forms also exist where its ubiquitination is catalyzed by a range of Ubiquitin E3 ligases from different classes. Therefore, experimentally S5a/Angiocidin may act as a useful substrate to monitor the activity of (E3) ligases, independent of their specific mechanisms of action (6). In cancer biology, where it is often referred to as Angiocidin, it is shown to slow tumor progression. It is found in the extracellular matrix of certain tumor subtypes, and it may act by suppressing angiogenesis or by directly inhibiting tumor cell growth (7,8). It also is found in several biological fluids where it is known primarily as ASF. It suppresses fluid secretion in response to enterotoxin and may act as an anti-inflammatory factor (9-11).
  • References:
    1. Nilsson, L.A. et al. (1992) J. Parasitol. 78:1055.
    2. Young, P. et al. (1998) J. Biol. Chem. 273:5461.
    3. Zhou, J. et al. (2004) J. Cell. Biochem. 92:125.
    4. Walters, K.J. et al. (2002) Biochemistry 41:1767.
    5. Rani, N. et al. (2011) Nature Commun. 3:749.
    6. Kim, H.T. & A.L. Goldberg (2012) Methods Mol. Biol. 832:653.
    7. Sabherwal, Y. et al. (2006) Exp. Cell Res. 312:2443.
    8. Godek, J. et al. (2011) Exp. Mol. Pathol. 90:244.
    9. Davidson, T.S. & W.J. Hickey (2004) J. Leukoc. Biol. 76:835.
    10. Davidson, T.S. & W.J. Hickey (2004) Lab. Invest. 84:307.
    11. Ulgheri, C. et al. (2010) Nutr. Res. Rev. 23:300.
  • Long Name:
    26S Proteasome Regulatory Subunit S5a
  • Entrez Gene IDs:
    5710 (Human); 19185 (Mouse); 83499 (Rat)
  • Alternate Names:
    Angiocidin; ASF; Macropain; Mcb1; PSMD4; pUB-R5; Rpn10 ; S5a
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. S5a binds to death receptor-6 to induce THP-1 monocytes to differentiate through the activation of the NF-kappaB pathway.
    Authors: Wang Z, Fan C, Zhou H, Lu J, Sun M, Song J, Le Y, Jiang H, Wang L, Jiao B
    J Cell Sci, 2014;127(0):3257-68.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
Staining Reagents
Description Application Cat# Citations Images  

Streptavidin-Phycoerythrin

Flow F0040 7
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Streptavidin-Alkaline Phosphatase

ELISA(Det) AR001 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

NorthernLights Streptavidin NL557

Flow, IHC, ICC NL999 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

NorthernLights Streptavidin NL637

Flow, IHC, ICC NL998 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

NorthernLights Streptavidin NL493

Flow, IHC, ICC NL997 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Streptavidin-Allophycocyanin

Flow F0050 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Avidin-Fluorescein

Flow F0030 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Supplemental Cell Selection Products
Description Application Cat# Citations Images  

MagCellect Streptavidin Ferrofluid

MAG999B 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

MagCellect Streptavidin Ferrofluid

MAG999 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Supplemental ELISA Products
Description Application Cat# Citations Images  

Streptavidin-HRP

ELISA DY998 44
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

EvenCoat Streptavidin Coated Plates

ELISA CP004
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

EvenCoat Streptavidin Coated Plates, 5 Pack

ELISA CP003 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Recombinant Human S5a/Angiocidin Biotin Protein, CF?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

    Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Feedback

Customer Information