hh Recombinant Human SCF GMP Protein, CF: R&D Systems

Recombinant Human SCF GMP Protein, CF

Animal-Free.
  
  • Purity
    >97%, by SDS-PAGE with silver staining, under reducing conditions. Intact mass analysis of recombinant human SCF confirms the predicted molecular mass of 18589 Da.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 1‑5 ng/mL.

    The specific activity of recombinant human SCF is approximately 564 units/μg, which is calibrated against recombinant human SCF WHO Standard (NIBSC code: 91/682).
  • Source
    E. coli-derived Glu26-Ala189, with an N-terminal Met Produced using non-animal reagents in an animal-free laboratory.
    Manufactured and tested under cGMP guidelines.
  • Accession #
  • N-terminal Sequence
    Analysis
    Met-Glu26-Gly-Ile-(Cys)-Arg-Asn-Arg-Val-Thr
  • Predicted Molecular Mass
    18.6 kDa
  • SDS-PAGE
    19 kDa, reducing conditions
255-GMP
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
Data Images
GMP-grade Recombinant Human SCF/c-kit Ligand (Catalog # 255-GMP) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 1-5 ng/mL.
1 μg/lane of GMP-grade Recombinant Human SCF/c-kit Ligand (Catalog #
255-GMP) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 19 kDa.
Background: SCF/c-kit Ligand

Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28‑40 kDa type I transmembrane glycoprotein (1). It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors (1‑7). SCF is a primary growth and activation factor for mast cells and eosinophils (8). Mature human SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail (9). The ECD shows both N‑linked and O-linked glycosylation (10). Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel-dickie mutant mice (11, 12). An alternately spliced isoform of human SCF lacks 28 aa that encompasses the primary proteolytic recognition site (13). Within the ECD of the long isoform (corresponding to this recombinant protein), human SCF shares 79%‑87% aa sequence identity with canine, feline, mouse, and rat SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells (9). Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/c‑kit to trigger receptor dimerization and signaling (14). SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels (15).

  • References:
    1. Ashman, L.K. (1999) Int. J. Biochem. Cell Biol. 31:1037. 
    2. Sette, C. et al. (2000) Int. J. Dev. Biol. 44:599. 
    3. Yoshida, H. et al. (2001) J. Invest. Dermatol. Symp. Proc. 6:1. 
    4. Erlandsson, A. et al. (2004) Exp. Cell Res. 301:201. 
    5. Kapur, R. et al. (2002) Blood 100:1287. 
    6. Wang, C.-H. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540. 
    7. Bashamboo, A. et al. (2006) J. Cell Sci. 119:3039. 
    8. Reber, L. et al. (2006) Eur. J. Pharmacol. 533:327.
    9. Martin, F.H. et al. (1990) Cell 63:203.
    10. Arakawa, T. et al. (1991) J. Biol. Chem. 266:18942.
    11. Majumdar, M.K. et al. (1994) J. Biol. Chem. 269:1237.
    12. Brannan, C.I. et al. (1991) Proc. Natl. Acad. Sci. 88:4671.
    13. Anderson, D.M. et al. (1991) Cell Growth Differ. 2:373.
    14. Lemmon, M.A. et al. (1997) J. Biol. Chem. 272:6311.
    15. Kanellakis, P. et al. (2006) Cardiovasc. Res. 70:117.
  • Long Name:
    Stem Cell Factor
  • Entrez Gene IDs:
    4254 (Human); 17311 (Mouse); 60427 (Rat); 403507 (Canine); 493937 (Feline)
  • Alternate Names:
    c-kit Ligand; DKFZp686F2250; familial progressive hyperpigmentation 2; FPH2; KIT ligand; Kitl; KITLG; KL-1; Mast cell growth factor; MGF; MGFSHEP7; SCF; SCFStem cell factor; SFc-Kit ligand; SLF; steel factor

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