Recombinant Human SMOC-1 Protein, CF

Catalog # Availability Size / Price Qty
6074-SM-050
Product Details
Citations (1)
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Recombinant Human SMOC-1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells.

When 5 x 104 cells/well are added to rhSMOC-1 coated plates, cell adhesion is enhanced in a dose dependent manner after 75 minutes at 37 °C. The ED50 for this effect is 0.5-2 μg/mL.

Source
Mouse myeloma cell line, NS0-derived human SMOC-1 protein
His27-Val434, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
His27 & Ile36
Predicted Molecular Mass
46.1 kDa
SDS-PAGE
60-65 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6074-SM

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: SMOC-1

SMOC-1 (secreted, or SPARC-related, modular calcium-binding protein 1) is a 70 - 90 kDa secreted glycoprotein that is a member of the SPARC family of matricellular molecules (1). Mature human SMOC-1 is 408 amino acids (aa) in length. It contains one Kazal-like domain (aa 42 - 87), two thyroglobulin type-1 (TY) segments (aa 92 - 159 and 224 - 292) and two EF-hand sequences (aa 359 - 394 and 396 - 431). One potential splice variant contains a 16 aa region of alternate sequence between the TY domains, a 7 aa deletion between the TY and EF-hand domains, and three alternate aa at the C-terminus. Mature human SMOC-1 shares 92% aa identity with mouse and rat SMOC-1 and 97%, 96% and 95% aa identity with canine, bovine and porcine SMOC-1, respectively. The principal difference between rodents and other mammals is an additional 19 aa near the C-terminus of rodent SMOC-1. Like other matricellular proteins, SMOC-1 is primarily expressed in basement membranes, although it has also been found in other extracellular matrices and the oocyte zona pellucida (1). It is present early in mouse embryogenesis, and is produced by cells deriving from all three germ layers (4). Recombinant bacterially produced human SMOC-1 and SMOC-2 were both shown to bind the acute phase protein, C-reactive protein, and the adhesion proteins, fibulin and vitronectin (2). A signaling role for SMOC-1 was shown in rat mesangial cells: induction of nitric oxide in response to inflammatory cytokines downregulates SMOC-1 which, in turn, downregulates expression of TGF-beta and TGF-beta -regulated genes. This mechanism is proposed to limit the profibrotic effects of TGF-beta, for example in the glomerulus (5). In Xenopus, the SMOC paralog has been shown to antagonize BMP activity.

References
  1. Vannahme, C. et al. (2002) J. Biol. Chem. 277:37977.
  2. Novinec, M. et al. (2008) Protein Expr. Purif. 62:75.
  3. Entrez Q8BLY1 and EDL02699.
  4. Gersdorff, N. et al. (2006) Histochem. Cell Biol. 126:705.
  5. Dreieicher, E. et al. (2009) J. Am. Soc. Nephrol. 20:1963.
  6. Thomas, J.T. et al. (2009) J. Biol. Chem. 284:18994.
Long Name
Secreted Modular Calcium-binding Protein 1
Entrez Gene IDs
64093 (Human); 64075 (Mouse); 314280 (Rat)
Alternate Names
Secreted modular calcium-binding protein 1; SMOC1; SMOC-1; SPARC related modular calcium binding 1; SPARC-related modular calcium-binding protein 1

Citation for Recombinant Human SMOC-1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. SMOC Binds to Pro-EGF, but Does Not Induce Erk Phosphorylation via the EGFR
    Authors: JT Thomas, L Chhuy-Hy, KR Andrykovic, M Moos
    PLoS ONE, 2016;11(4):e0154294.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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