Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus, CF

New rhShh Available! C-term cholesterol, N-term fatty acid-modified; 250-fold activity!
New Product 8908-SH/CF.
(14 citations)   
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465. The ED50 for this effect is 0.1-0.4 µg/mL.
  • Source
    E. coli-derived Cys24-Gly197 (Cys24Ile-Ile), with an N-terminal Met
  • Accession #
  • N-terminal Sequence
    Analysis
    Met
  • Predicted Molecular Mass
    20 kDa
  • SDS-PAGE
    22 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1845-SH/CF
 
1845-SH
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus (Catalog #
1845-SH/CF) induces alkaline phosphatase production by the C3H10T1/2 mouse embryonic fibroblast cell line. The ED50 for this effect is 0.1-0.4 μg/mL.
1 μg/lane of Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 22 kDa.
Mass Spectrometry
MALDI-TOF analysis of Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus. The major peak corresponds to the calculated molecular mass, 19814 Da. The minor peak at 20029  is a matrix-associated artifact of the MALDI-TOF.
Background: Sonic Hedgehog/Shh
Sonic Hedgehog (Shh) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation as well as T cell determination. In adult tissue Shh is associated with cancer development and tissue remodeling following injury (1-3). Human Shh encodes a 462 amino acid (aa) precursor protein that is autocatalytically processed to yield a non-glycosylated 19 kDa N-terminal fragment (Shh-N) and a glycosylated 25 kDa C-terminal protein (Shh-C) (4). Shh-C, which is responsible for the intramolecular processing of Shh, is rapidly degraded following Shh proteolysis (5). Shh-N is highly conserved, sharing >98% aa identity between mouse, human, rat, canine, porcine, and chicken Shh-N. Shh-N can be palmitoylated at its
N-terminal cysteine and modified by cholesterol addition at its C-terminus (6). These modifications contribute to the membrane tethering of Shh as well as its assembly into various sized multimers (6-9). Lipid modification and multimerization greatly increase Shh-N receptor binding affinity and signaling potency (5, 6, 8, 9). Monomeric and multimeric Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17 (10-12). Modifications also extend the effective range of Shh functionality and are required for the development of protein gradients important in tissue morphogenesis (9, 13). Canonical signaling of Shh is mediated by a multicomponent receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO) (14). The binding of Shh to PTCH releases the basal repression of SMO by PTCH. Shh activity can also be regulated through interactions with heparin, glypicans, and membrane-associated Hip (hedgehog interacting protein) (13, 15, 16).
  • References:
    1. Briscoe, J. and P.P. Therond (2013) Mol. Cell. Biol. 14:416.
    2. Aviles, E.C. et al. (2013) Front. Cell. Neurosci. 7:86.
    3. Xie, J. et al. (2013) OncoTargets Ther. 6:1425.
    4. Marigo, V. et al. (1995) Genomics 28:44.
    5. Zeng, X. et al. (2001) Nature 411:716.
    6. Feng, J. et al. (2004) Development 131:4357.
    7. Goetz, J.A. et al. (2006) J. Biol. Chem. 281:4087.
    8. Pepinsky, R.B. et al. (1998) J. Biol. Chem. 273:14037.
    9. Chen, M.-H. et al. (2004) Genes Dev. 18:641.
    10. Etheridge, L.A. et al. (2010) Development 137:133.
    11. Jakobs, P. et al. (2014) J. Cell Sci. 127:1726.
    12. Dierker, T. et al. (2009) J. Biol. Chem. 284:8013.
    13. Lewis, P.M. et al. (2001) Cell 105:599.
    14. Carpenter, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:13630.
    15. Filmus, J. and M. Capurro (2014) Matrix Biol. 35:248.
    16. Chuang, P.-T. and A.P. McMahon (1999) Nature 397:617.
  • Entrez Gene IDs:
    6469 (Human); 20423 (Mouse)
  • Alternate Names:
    HHG1; HHG-1; HLP3; HPE3; MCOPCB5sonic hedgehog (Drosophila) homolog; Shh; SMMCIsonic hedgehog homolog (Drosophila); sonic hedgehog homolog; sonic hedgehog protein; Sonic Hedgehog; TPT; TPTPS
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. GLI1 blockade potentiates the antitumor activity of PI3K antagonists in lung squamous cell carcinoma
    Authors: S Kasiri, C Shao, B Chen, AN Wilson, P Yenerall, BC Timmons, L Girard, H Tian, C Behrens, II Wistuba, AF Gazdar, J Kim
    Cancer Res., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Developmental Reprogramming in Mesenchymal Stromal Cells of Human Subjects with Idiopathic Pulmonary Fibrosis
    Sci Rep, 2016;6(0):37445.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. Hedgehog Acyltransferase as a target in estrogen receptor positive, HER2 amplified, and tamoxifen resistant breast cancer cells.
    Authors: Matevossian A, Resh M
    Mol Cancer, 2015;14(0):72.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. Inversion of Sonic hedgehog action on its canonical pathway by electrical activity.
    Authors: Belgacem Y, Borodinsky L
    Proc Natl Acad Sci U S A, 2015;112(13):4140-5.
    Species: Xenopus
    Sample Type: Embryo
    Application: Bioassay
  5. Hedgehog signaling stimulates the conversion of cholesterol to steroids.
    Authors: Tang C, Pan Y, Luo H, Xiong W, Zhu H, Ruan H, Wang J, Zou C, Tang L, Iguchi T, Long F, Wu X
    Cell Signal, 2015;27(3):487-97.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  6. Mutant astrocytes differentiated from Rett syndrome patients-specific iPSCs have adverse effects on wild-type neurons.
    Authors: Williams E, Zhong X, Mohamed A, Li R, Liu Y, Dong Q, Ananiev G, Mok J, Lin B, Lu J, Chiao C, Cherney R, Li H, Zhang S, Chang Q
    Hum Mol Genet, 2014;23(11):2968-80.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  7. Physiological characterisation of human iPS-derived dopaminergic neurons.
    Authors: Hartfield, Elizabet, Yamasaki-Mann, Michiko, Ribeiro Fernandes, Hugo J, Vowles, Jane, James, William, Cowley, Sally A, Wade-Martins, Richard
    PLoS ONE, 2014;9(2):e87388.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  8. Chondrocyte proliferation regulated by secreted luminal domain of ER stress transducer BBF2H7/CREB3L2.
    Authors: Saito A, Kanemoto S, Zhang Y, Asada R, Hino K, Imaizumi K
    Mol Cell, 2014;53(1):127-39.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Bioassay
  9. BMP and TGF-beta pathway mediators are critical upstream regulators of Wnt signaling during midbrain dopamine differentiation in human pluripotent stem cells.
    Authors: Cai J, Schleidt S, Pelta-Heller J, Hutchings D, Cannarsa G, Iacovitti L
    Dev Biol, 2013;376(1):62-73.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  10. Better prognosis of patients with glioma expressing FGF2-dependent PDGFRA irrespective of morphological diagnosis.
    Authors: Chen, Dongfeng, Persson, Annette, Sun, Yingyu, Salford, Leif G, Nord, David Gi, Englund, Elisabet, Jiang, Tao, Fan, Xiaolong
    PLoS ONE, 2013;8(4):e61556.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  11. Medial ganglionic eminence-like cells derived from human embryonic stem cells correct learning and memory deficits.
    Authors: Liu Y, Weick J, Liu H, Krencik R, Zhang X, Ma L, Zhou G, Ayala M, Zhang S
    Nat Biotechnol, 2013;31(5):440-7.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  12. A novel synthetic smoothened antagonist transiently inhibits pancreatic adenocarcinoma xenografts in a mouse model.
    Authors: Strand MF, Wilson SR, Dembinski JL, Holsworth DD, Khvat A, Okun I, Petersen D, Krauss S
    PLoS ONE, 2011;6(6):e19904.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  13. Segregation of ipsilateral retinal ganglion cell axons at the optic chiasm requires the Shh receptor Boc.
    Authors: Fabre PJ, Shimogori T, Charron F
    J. Neurosci., 2010;30(0):266.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  14. Human embryonic stem cells in culture possess primary cilia with hedgehog signaling machinery.
    Authors: Kiprilov EN, Awan A, Desprat R, Velho M, Clement CA, Byskov AG, Andersen CY, Satir P, Bouhassira EE, Christensen ST, Hirsch RE
    J. Cell Biol., 2008;180(5):897-904.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
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FAQs

  1. What is the difference between Recombinant Human Sonic Hedgehog Catalog # 1845-SH and Catalog # 1314-SH?

    • Recombinant Human Sonic Hedgehog, Catalog # 1845-SH, possesses a N-terminal mutation that increases its potency in bioassay tests. The amino acid sequence is Cys24-Gly197 (Cys24Ile-Ile), accession number NP_000184. The Cys24Ile-Ile mutation was created to match a publication that describes enhanced activity with these modifications: "http://www.ncbi.nlm.nih.gov/pubmed/11284692". Catalog # 1314-SH does not possess this mutation.

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