Recombinant Human SorCS1 Protein, CF

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3457-SR-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human SorCS1 Protein, CF Summary

Product Specifications

Purity
>85%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized rhSorCS1 at 1 µg/mL (100 µL/well) can bind rh beta -NGF with an apparent KD <75 nM.
Source
Mouse myeloma cell line, NS0-derived human SorCS1 protein
Ser111-Ser1099 (Ser231Gly), with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ser111
Predicted Molecular Mass
112 kDa
SDS-PAGE
130-140 kDa, reducing conditions

Product Datasheets

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3457-SR

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3457-SR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: SorCS1

SorCS1 is a type I transmembrane receptor of the mammalian Vps10p (vacuolar protein-sorting 10 protein) family (1, 2). These sorting receptors include sortilin, SorLA, and three SorCS proteins. Three splicing variants (SorCS1a, b and c) differ only in their cytoplasmic domains (3). All variants are predominantly expressed in the central nervous system, but SorCS1 can also be identified in heart, kidney and pancreatic islets (2 - 5). SorCS1a mediates endocytosis, and only ~10% of it is expressed on the cell surface. SorCS1b shows higher surface expression (~45%) and is much less involved in endocytosis. SorCS1c is intermediate. Human SorCS1a is synthesized as a 1159 amino acid (aa) preproform with a 33 aa signal sequence and a 77 aa propeptide. After proteolytic processing at a furin-type consensus sequence, the mature SorCS1a is a 1049 aa, 130 kDa protein with a 989 aa extracellular/lumenal domain (ECD). Within the ECD, human SorCS1 shares 93%, 94%, 93% and 98% aa identity with mouse, rat, bovine and canine SorCS1, respectively. It also shares 70% and 46% aa identity with the ECD of human SorCS3 and SorCS2, respectively. The ECD contains an imperfect leucine-rich repeat (LRR) and a Vps10p domain and binds the growth factor PDGF-BB (1, 2, 6). Expression in the hippocampus indicates that SorCS1 may modulate PDGF-BB activity in this location (6). SorCS1 has also been identified as a susceptibility gene for type 2 diabetes in overweight females (4). Consequently, it has been proposed to affect insulin secretion by modifying PDGF-mediated growth of the islet vasculature (7). The 80 kDa ECD may be constitutively or inducibly shed, mainly via the metalloproteinase TACE/ADAM17 (6). The shed soluble form also binds PDGF. The cellular portion appears to undergo regulated intramembrane proteolysis (8).

References
  1. Hampe, W. et al. (2001) Hum. Genet. 108:529.
  2. Hermey, G. et al. (1999) Biochem. Biophys. Res. Commun. 266:347.
  3. Hermey, G. et al. (2003) J. Biol. Chem. 278:7390.
  4. Clee, S.M. et al. (2006) Nat. Genet. 38:688.
  5. Hermey, G. et al. (2001) Neurosci. Lett. 313:83.
  6. Hermey, G. et al. (2006) Biochem. J. 395:285.
  7. Goodarzi, M.O. et al. (2007) Diabetes Apr 10 [Epub ahead of print].
  8. Nyborg, A.C. et al. (2006) Mol. Neurodegen. 1:3.
Long Name
Sortilin-related VPS10 Domain Containing Receptor 1
Entrez Gene IDs
114815 (Human); 58178 (Mouse); 309533 (Rat)
Alternate Names
FLJ41758; FLJ43475; FLJ44957; hSorCS; SORCS receptor 1; SORCS; SorCS1; sortilin-related VPS10 domain containing receptor 1; VPS10 domain receptor protein SORCS 1; VPS10 domain receptor SorCS; VPS10 domain-containing receptor SorCS1

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