Recombinant Human Stanniocalcin 2/STC-2 Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 60-360 ng/mL in the presence of 10 ng/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
  • Source
    Mouse myeloma cell line, NS0-derived Thr25-Arg302, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Thr25
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    31 kDa
  • SDS-PAGE
    34-43 kDa, reducing conditions
9405-SO
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human Stanniocalcin 2/STC-2 (Catalog # 9405-SO) inhibits Recombinant Mouse Wnt-3a induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 60-360 ng/ml in the presence of 10 ng/ml of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
Background: Stanniocalcin 2/STC-2
Stanniocalcin 2 (STC-2) is a secreted disulfide-linked homodimeric glycoprotein hormone that is related to the STC protein first discovered from corpuscles of stannius in fish (1). The STC-2 has 10 of its 15 cysteine residues conserved among stanniocalcin family members and is phosphorylated by casein kinase 2 exclusively on its serine residues (2). Its C-terminus contains a cluster of histidine residues which may interact with metal ions (1). STC-2 is expressed in a wide variety of tissues. In the ovary, STC-2 has been shown to be a paracrine hormone that regulates granulosa cell function (1). The amino acid sequence of human mature STC-2 is 36% identical to that of human STC-1. It is also 99% and 88% identical to that of monkey and mouse STC-2, respectively (3). STC-2 enhances mesenchymal stem cell survival (4), promotes cell proliferation in cervical cancer (5), suppresses breast cancer cell migration and invasion (6), promotes osteoblast differentiation (7), and inhibits longitudinal bone growth directly at the growth plate (8). It is also a biomarker for cervical and lung cancers (9, 10),
  • References:
    1. Luo, C. et al. (2005). Endocrinology 146:469.
    2. Tagliabracci, V. S. et al. (2015). Cell 161:1619.
    3. Ishibashi, K. et al. (1998). Biochem. Biophys. Res. Commun. 250:252.
    4. Kim PH, et al. (2015) BMB Rep. 48:702.
    5. Wang Y, et al. (2015) Biochem Biophys Res Commun. 466:362.
    6. Hou J, et al. (2015) PLoS One. 10:e0122179.
    7. Zhou J, et al. (2016) Mol Med Rep. 14:5653.
    8. Wu S, et al. (2006) J Biol Chem. 281: 5120.
    9. Shen XJ, et al. (2014) Int J Clin Exp Pathol. 7: 8770.
    10. Na SS, et al. (2015) Biochim Biophys Acta. 1854:668.
  • Entrez Gene IDs:
    8614 (Human); 20856 (Mouse)
  • Alternate Names:
    Stanniocalcin 2; stanniocalcin-2; STC2; STC-2; STC-2Stanniocalcin-related protein; STC-related protein; STCRP
Related Research Areas
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Flow, AP MAB050R  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag APC-conjugated Antibody

ICFlow IC050A  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag PE-conjugated Antibody

ICFlow IC050P 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Biotinylated Antibody

WB BAM050 3  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag PerCP-conjugated Antibody

ICFlow IC050C  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC0501G
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 594-conjugated Antibody

ICFlow IC050T
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 647-conjugated Antibody

ICFlow IC0501R
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 405-conjugated Antibody

ICFlow IC050V
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 647-conjugated Antibody

ICFlow IC050R
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 750-conjugated Antibody

ICFlow IC0501S
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 405-conjugated Antibody

ICFlow IC0501V
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 350-conjugated Antibody

ICFlow IC050U
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 750-conjugated Antibody

ICFlow IC050S
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 350-conjugated Antibody

ICFlow IC0501U
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC0501N
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 594-conjugated Antibody

ICFlow IC0501T
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Recombinant Human Stanniocalcin 2/STC-2 Protein, CF?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

    Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Feedback

Customer Information