Recombinant Human Stanniocalcin 2/STC-2 Protein, CF

Catalog # Availability Size / Price Qty
9405-SO-050
Recombinant Human Stanniocalcin 2/STC-2 Protein, CF Bioactivity
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Recombinant Human Stanniocalcin 2/STC-2 Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 60-360 ng/mL in the presence of 10 ng/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
Source
Mouse myeloma cell line, NS0-derived human Stanniocalcin 2/STC-2 protein
Thr25-Arg302, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Thr25
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
31 kDa
SDS-PAGE
34-43 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9405-SO

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Bioactivity View Larger

Recombinant Human Stanniocalcin 2/STC-2 (Catalog # 9405-SO) inhibits Recombinant Mouse Wnt-3a induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 60-360 ng/ml in the presence of 10 ng/ml of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Stanniocalcin 2/STC-2

Stanniocalcin 2 (STC-2) is a secreted disulfide-linked homodimeric glycoprotein hormone that is related to the STC protein first discovered from corpuscles of stannius in fish (1). The STC-2 has 10 of its 15 cysteine residues conserved among stanniocalcin family members and is phosphorylated by casein kinase 2 exclusively on its serine residues (2). Its C-terminus contains a cluster of histidine residues which may interact with metal ions (1). STC-2 is expressed in a wide variety of tissues. In the ovary, STC-2 has been shown to be a paracrine hormone that regulates granulosa cell function (1). The amino acid sequence of human mature STC-2 is 36% identical to that of human STC-1. It is also 99% and 88% identical to that of monkey and mouse STC-2, respectively (3). STC-2 enhances mesenchymal stem cell survival (4), promotes cell proliferation in cervical cancer (5), suppresses breast cancer cell migration and invasion (6), promotes osteoblast differentiation (7), and inhibits longitudinal bone growth directly at the growth plate (8). It is also a biomarker for cervical and lung cancers (9, 10),

References
  1. Luo, C. et al. (2005). Endocrinology 146:469.
  2. Tagliabracci, V. S. et al. (2015). Cell 161:1619.
  3. Ishibashi, K. et al. (1998). Biochem. Biophys. Res. Commun. 250:252.
  4. Kim PH, et al. (2015) BMB Rep. 48:702.
  5. Wang Y, et al. (2015) Biochem Biophys Res Commun. 466:362.
  6. Hou J, et al. (2015) PLoS One. 10:e0122179.
  7. Zhou J, et al. (2016) Mol Med Rep. 14:5653.
  8. Wu S, et al. (2006) J Biol Chem. 281: 5120.
  9. Shen XJ, et al. (2014) Int J Clin Exp Pathol. 7: 8770.
  10. Na SS, et al. (2015) Biochim Biophys Acta. 1854:668.
Entrez Gene IDs
8614 (Human); 20856 (Mouse)
Alternate Names
Stanniocalcin 2; stanniocalcin-2; STC2; STC-2; STC-2Stanniocalcin-related protein; STC-related protein; STCRP

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