>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 60-360 ng/mL in the presence of 10 ng/mL of
Mouse Wnt‑3a (Catalog # 1324-WN).
Mouse myeloma cell line, NS0-derived Thr25-Arg302, with a C-terminal 6-His tag
Recombinant Human Stanniocalcin 2/STC-2 (Catalog # 9405-SO) inhibits Recombinant Mouse Wnt-3a induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 60-360 ng/ml in the presence of 10 ng/ml of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
Background: Stanniocalcin 2/STC-2
Stanniocalcin 2 (STC-2) is a secreted disulfide-linked homodimeric
glycoprotein hormone that is related to the STC protein first discovered from corpuscles
of stannius in fish (1). The STC-2 has 10 of its 15 cysteine residues conserved among stanniocalcin
family members and is phosphorylated by casein kinase 2 exclusively on its
serine residues (2). Its C-terminus contains a cluster of histidine residues
which may interact with metal ions (1). STC-2
is expressed in a wide variety of tissues. In the ovary, STC-2 has been shown
to be a paracrine hormone that regulates granulosa cell function (1). The amino acid
sequence of human mature STC-2 is 36% identical to that of human STC-1. It is
also 99% and 88% identical to that of monkey and mouse STC-2, respectively
(3). STC-2 enhances mesenchymal stem cell survival (4), promotes cell proliferation
in cervical cancer (5), suppresses breast cancer cell migration and invasion (6),
promotes osteoblast differentiation (7), and inhibits longitudinal bone growth
directly at the growth plate (8). It is also a biomarker for cervical and lung
cancers (9, 10),
Luo, C. et al. (2005). Endocrinology 146:469.
Tagliabracci, V. S. et al. (2015). Cell 161:1619.
Ishibashi, K. et al. (1998). Biochem. Biophys. Res. Commun. 250:252.
Kim PH, et al. (2015) BMB Rep. 48:702.
Wang Y, et al. (2015) Biochem Biophys Res Commun. 466:362.
Hou J, et al. (2015) PLoS One. 10:e0122179.
Zhou J, et al. (2016) Mol Med Rep. 14:5653.
Wu S, et al. (2006) J Biol Chem. 281: 5120.
Shen XJ, et al. (2014) Int J Clin Exp Pathol. 7: 8770.
Na SS, et al. (2015) Biochim Biophys Acta. 1854:668.
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