Recombinant Human SUMO E1 (SAE1/UBA2) Protein, CF
Discontinued Product
Recombinant Human SUMO E1 (SAE1/UBA2) Protein, CF Summary
Product Specifications
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E-315
| Formulation | Supplied as a solution in HEPES, NaCl, DTT and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: SUMO Activating Enzyme E1 (SAE1/UBA2)
Small Ubiquitin-like Modifier (SUMO) Activating Enzyme Subunit 1 (SAE1) is the highly conserved human ortholog of yeast Ubiquitin-activating enzyme E1-like (UBA2) (1). These SUMO-activating (E1) enzymes are critical for the enzymatic attachment of SUMO molecules to a target protein by a post-translational modification process termed SUMOylation (2-4). The ATP-dependent E1 enzyme charges the SUMO by forming a high-energy thiol ester intermediate which is transferred to the UBE2I/Ubc9 SUMO-conjugating (E2) enzyme (5,6). The second step is the trans-esterification reaction whereby SUMO is transferred to Cys93 of UbcH9. UBE2I/Ubc9 is the only known E2 that is able to mediate the conjugation of SUMO to lysine residues on a variety of cellular targets, usually in the absence of a Ubiquitin ligase (E3). Although UBE2I/Ubc9 can directly recognize and modify lysine residues contained in a SUMOylation motif, E3-like factors most likely facilitate the SUMOylation of specific substrates.
Conjugation of the ubiquitin-like modifier SUMO (Sentrin) requires the activities of the heterodimeric E1 (SAE1/UBA2) and the UbcH9 E2 enzyme. The dimeric activating enzyme utilizes ATP to adenylate the C-terminal glycine residue of all SUMO proteins, forming a high energy thiolester bond with the cysteine residue of UBA2 and the concomitant release of AMP and PPi. The second step is the trans-esterification reaction whereby SUMO is transferred to Cys93 of UbcH9.
- Johnson, E.S. et al. (1997) EMBO J. 16:5509.
- Desterro, J.M. et al. (1997) FEBs. Lett. 417:297.
- Bettermann, K. et al. (2012) Cancer Lett. 316:113.
- Praefcke, G.J. et al. (2012) Trends Biochem. Sci. 37:23.
- Okuma, T. et al. (1999) Biochem. Biophys. Res. Commun. 254:693.
- Tatham, M.H. et al. (2001) J. Biol. Chem. 276:35368.
Citations for Recombinant Human SUMO E1 (SAE1/UBA2) Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
9
Citations: Showing 1 - 9
Filter your results:
Filter by:
-
KSHV hijacks the antiviral kinase IKK? to initiate lytic replication
Authors: Wang, X;Liu, Z;Xu, X;Wang, X;Ming, Z;Liu, C;Gao, H;Li, T;Liang, Q;
PLoS pathogens
Species: N/A
Sample Types: Recombinant Protein
Applications: Bioassay -
Concerted SUMO-targeted ubiquitin ligase activities of TOPORS and RNF4 are essential for stress management and cell proliferation
Authors: Liu, J;Ackermann, L;Hoffmann, S;Gál, Z;Hendriks, I;Jain, C;Morlot, L;Tatham, M;McLelland, G;Hay, R;Nielsen, M;Brummelkamp, T;Haahr, P;Mailand, N;
Nature Structural & Molecular Biology
Species: N/A
Sample Types: Recombinant Protein
Applications: Bioassay -
RSK1 SUMOylation is required for KSHV lytic replication
Authors: Z Liu, C Liu, X Wang, W Li, J Zhou, P Dong, MZX Xiao, C Wang, Y Zhang, J Fu, F Zhu, Q Liang
PloS Pathogens, 2021-12-06;17(12):e1010123.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Mechanism of Adenovirus E4-ORF3-Mediated SUMO Modifications
Authors: SY Sohn, P Hearing
MBio, 2019-02-26;10(1):.
Species: Human
Sample Types: Recombinant Protein
Applications: Enzyme Assay -
TRIM28 promotes HIV-1 latency by SUMOylating CDK9 and inhibiting P-TEFb
Authors: X Ma, T Yang, Y Luo, L Wu, Y Jiang, Z Song, T Pan, B Liu, G Liu, J Liu, F Yu, Z He, W Zhang, J Yang, L Liang, Y Guan, X Zhang, L Li, W Cai, X Tang, S Gao, K Deng, H Zhang
Elife, 2019-01-17;8(0):.
Species: Human
Sample Types: Recombinant Protein
Applications: SUMOylation Assay -
Dynamic SUMO remodeling drives a series of critical events during the meiotic divisions in Caenorhabditis elegans
Authors: AC Davis-Roca, NS Divekar, RK Ng, SM Wignall
PLoS Genet., 2018-09-04;14(9):e1007626.
Species: C. elegans
Sample Types: Cell Lysates
Applications: Bioassay -
Sumoylation in p27kip1 via RanBP2 promotes cancer cell growth in cholangiocarcinoma cell line QBC939
Authors: J Yang, Y Liu, B Wang, H Lan, Y Liu, F Chen, J Zhang, J Luo
BMC Mol. Biol., 2017-09-07;18(1):23.
Species: Human
Sample Types: Protein
Applications: Bioassay -
The adenovirus E4-ORF3 protein functions as a SUMO E3 ligase for TIF-1? sumoylation and poly-SUMO chain elongation
Proc Natl Acad Sci USA, 2016-05-31;113(24):6725-30.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Human SUMO E1 (SAE1/UBA2) Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Human SUMO E1 (SAE1/UBA2) Protein, CF and earn rewards!
Have you used Recombinant Human SUMO E1 (SAE1/UBA2) Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
