Recombinant Human SUMO E1 (SAE1/UBA2) Protein, CF

Catalog # Availability Size / Price Qty
Product Details
Citations (6)

Recombinant Human SUMO E1 (SAE1/UBA2) Protein, CF Summary

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Recombinant Human SUMO E1 (SAE1/UBA2) is a member of the SUMO-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a SUMO-conjugating (E2) enzyme to conjugate SUMO to substrate proteins. A SUMO ligase (E3) is sometimes utilized for SUMO conjugation, but is not always required. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human SUMO E1 (SAE1/UBA2) concentration of 50-500 nM.
E. coli-derived human SUMO Activating Enzyme E1 (SAE1/UBA2) protein
Predicted Molecular Mass
38 kDa (SAE1) & 71 kDa (UBA2)

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Supplied as a solution in HEPES, NaCl, DTT and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: SUMO Activating Enzyme E1 (SAE1/UBA2)

Small Ubiquitin-like Modifier (SUMO) Activating Enzyme Subunit 1 (SAE1) is the highly conserved human ortholog of yeast Ubiquitin-activating enzyme E1-like (UBA2) (1). These SUMO-activating (E1) enzymes are critical for the enzymatic attachment of SUMO molecules to a target protein by a post-translational modification process termed SUMOylation (2-4).  The ATP-dependent E1 enzyme charges the SUMO by forming a high-energy thiol ester intermediate which is transferred to the UBE2I/Ubc9 SUMO-conjugating (E2) enzyme (5,6). The second step is the trans-esterification reaction whereby SUMO is transferred to Cys93 of UbcH9. UBE2I/Ubc9 is the only known E2 that is able to mediate the conjugation of SUMO to lysine residues on a variety of cellular targets, usually in the absence of a Ubiquitin ligase (E3). Although UBE2I/Ubc9 can directly recognize and modify lysine residues contained in a SUMOylation motif, E3-like factors most likely facilitate the SUMOylation of specific substrates.

Conjugation of the ubiquitin-like modifier SUMO (Sentrin) requires the activities of the heterodimeric E1 (SAE1/UBA2) and the UbcH9 E2 enzyme. The dimeric activating enzyme utilizes ATP to adenylate the C-terminal glycine residue of all SUMO proteins, forming a high energy thiolester bond with the cysteine residue of UBA2 and the concomitant release of AMP and PPi. The second step is the trans-esterification reaction whereby SUMO is transferred to Cys93 of UbcH9.

  1. Johnson, E.S. et al. (1997) EMBO J. 16:5509.
  2. Desterro, J.M. et al. (1997) FEBs. Lett. 417:297.
  3. Bettermann, K. et al. (2012) Cancer Lett. 316:113.
  4. Praefcke, G.J. et al. (2012) Trends Biochem. Sci. 37:23.
  5. Okuma, T. et al. (1999) Biochem. Biophys. Res. Commun. 254:693.
  6. Tatham, M.H. et al. (2001) J. Biol. Chem. 276:35368.
Long Name
SUMO1 Activating Enzyme E1
Entrez Gene IDs
10055 (Human); 56459 (Mouse); 308384 (Rat); 856310 (Yeast)
Alternate Names
AOS1; AOS1activator of SUMO1; FLJ3091; HSPC140; SAE1; SUA1; SUA1sentrin/SUMO-activating protein AOS1; SUMO Activating Enzyme E1 (SAE1/UBA2); SUMO-1 activating enzyme E1 N subunit; SUMO1 activating enzyme subunit 1; SUMO-1 activating enzyme subunit 1; SUMO-activating enzyme subunit 1; UBA2; Ubiquitin-like 1-activating enzyme E1A; ubiquitin-like protein SUMO-1 activating enzyme; UBLE1A

Citations for Recombinant Human SUMO E1 (SAE1/UBA2) Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
Filter your results:

Filter by:

  1. Mechanism of Adenovirus E4-ORF3-Mediated SUMO Modifications
    Authors: SY Sohn, P Hearing
    MBio, 2019;10(1):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Enzyme Activity
  2. TRIM28 promotes HIV-1 latency by SUMOylating CDK9 and inhibiting P-TEFb
    Authors: X Ma, T Yang, Y Luo, L Wu, Y Jiang, Z Song, T Pan, B Liu, G Liu, J Liu, F Yu, Z He, W Zhang, J Yang, L Liang, Y Guan, X Zhang, L Li, W Cai, X Tang, S Gao, K Deng, H Zhang
    Elife, 2019;8(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: SUMOylation Assay
  3. Dynamic SUMO remodeling drives a series of critical events during the meiotic divisions in Caenorhabditis elegans
    Authors: AC Davis-Roca, NS Divekar, RK Ng, SM Wignall
    PLoS Genet., 2018;14(9):e1007626.
    Species: Nematode (C. elegans)
    Sample Types: Cell Lysates
    Applications: Bioassay
  4. HSP70-Hrd1 axis precludes the oncorepressor potential of N-terminal misfolded Blimp-1s in lymphoma cells
    Authors: WF Wang, L Yan, Z Liu, LX Liu, J Lin, ZY Liu, XP Chen, W Zhang, ZZ Xu, T Shi, JM Li, YL Zhao, G Meng, Y Xia, JY Li, J Zhu
    Nat Commun, 2017;8(1):363.
    Applications: Bioassay
  5. Sumoylation in p27kip1 via RanBP2 promotes cancer cell growth in cholangiocarcinoma cell line QBC939
    Authors: J Yang, Y Liu, B Wang, H Lan, Y Liu, F Chen, J Zhang, J Luo
    BMC Mol. Biol., 2017;18(1):23.
    Species: N/A
    Sample Types: Proteins
    Applications: Bioassay
  6. The adenovirus E4-ORF3 protein functions as a SUMO E3 ligase for TIF-1? sumoylation and poly-SUMO chain elongation
    Proc Natl Acad Sci USA, 2016;113(24):6725-30.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay


No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human SUMO E1 (SAE1/UBA2) Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human SUMO E1 (SAE1/UBA2) Protein, CF and earn rewards!

Have you used Recombinant Human SUMO E1 (SAE1/UBA2) Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review