Recombinant Human Syndecan-3 Protein, CF Summary
Gln48-Lys383, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Reconstitution||Reconstitute at 200 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Syndecan-3, also called N-syndecan, is one of four vertebrate syndecans that are principal carriers of heparan sulfate and chondroitin sulfate glycosaminoglycans (GAGs) (1 - 3). These type 1 transmembrane proteins show conserved cytoplasmic domains and divergent extracellular domains (1 - 3). Human Syndecan-3 is synthesized as a 442 amino acid (aa) core protein with a 44 aa signal sequence, a 343 aa extracellular domain (ECD), a 21 aa transmembrane (TM) region and a 34 aa cytoplasmic tail with a binding site for PDZ domains (1). The ECD of human Syndecan-3 shares 83%, 83%, 92%, 91% and 91% aa identity with of mouse, rat, equine, bovine and canine Syndecan-3, respectively. Splice isoforms of 384 aa and 346 aa, containing either a 28 aa substitution for aa 1 - 86 or deletion of aa 1 - 96, have been reported (4). Syndecan-3 contains four conserved closely-spaced GAG attachment sites near the N-terminus and unique threonine-rich and mucin-like sequences near the membrane (4). Addition of glycan side chains results in an apparent size of 120 - 150 kDa. Non-covalent homodimerization of Syndecan-3 or, potentially, heterodimerization with Syndecan-2 or -4, is dependent on the transmembrane domain (5). A cleavage site near the TM domain allows shedding of soluble ECD; the remainder of the molecule undergoes regulated intramembrane proteolysis (6). Syndecan-3 is expressed in the nervous system and at limb buds during development (1, 2). It is expressed on neuronal axons and Schwann cell perinodal processes, promoting nerve cell migration and synapse formation (7, 8). Roles in memory and body weight regulation have been described (2, 9, 10). Through localization of growth factors such as FGF2, HGF and TGF-beta, it regulates expression of molecules important for differentiation of muscle and bone, such as myogenin, BMP-2 and hedgehog family members (1, 2, 11 - 13). In adults, it is upregulated during regeneration, such as following myocardial infarction (14).
- Tkachenko, E. et al. (2005) Circ. Res. 96:488.
- Reizes, O. et al. (2008) Int. J. Biochem. Cell Biol. 40:28.
- Carey, D.J. et al. (1997) J. Biol. Chem. 272:2873.
- ENTREZ protein Accession # O75056, EAX076736 and EAX07637.
- Dews, I.C. and K.R. MacKenzie (2007) Proc. Natl. Acad. Sci. USA 104:20782.
- Schultz, J.G. et al. (2003) J. Biol. Chem. 278:48651.
- Hienola, A. et al. (2006) J. Cell Biol. 174:569.
- Goutebroze, L. et al. (2003) BMC Neurosci. 4:29.
- Kaksonen, M. et al. (2002) Mol. Cell. Neurosci. 21:158.
- Strader. A.D. et al. (2004) J. Clin. Invest. 114:1354.
- Cornelison, D.D.W. et al. (2004) Genes Dev. 18:2231.
- Fisher, M.C. et al. (2006) Matrix Biol. 25:27.
- Pacifici, M. et al. (2005) J. Bone Miner. Metab. 23:191.
- Finsen, A.V. et al. (2004) Physiol. Genomics 16:301.
Citation for Recombinant Human Syndecan-3 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Soluble syndecan-3 binds chemokines, reduces leukocyte migration in vitro and ameliorates disease severity in models of rheumatoid arthritis
Authors: AD Eustace, EF McNaughton, S King, O Kehoe, A Kungl, D Mattey, AH Nobbs, N Williams, J Middleton
Arthritis Res. Ther., 2019;21(1):172.
Sample Types: In Vivo
Applications: In Vivo
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