Recombinant Human Syndecan-3 Protein, CF

Catalog # Availability Size / Price Qty
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Citations (1)
Supplemental Products
Reviews (1)

Recombinant Human Syndecan-3 Protein, CF Summary

Product Specifications

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Measured by the ability of the immobilized protein to inhibit the adhesion of Saos‑2 human osteosarcoma cells to human Fibronectin. The ED50 for this effect is 0.75-4.5 μg/mL.
Mouse myeloma cell line, NS0-derived human Syndecan-3 protein
Gln48-Lys383, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
No results obtained: Gln48 predicted
Predicted Molecular Mass
35.5 kDa
100-150 kDa, reducing conditions

Product Datasheets

You must select a language.



Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: Syndecan-3

Syndecan-3, also called N-syndecan, is one of four vertebrate syndecans that are principal carriers of heparan sulfate and chondroitin sulfate glycosaminoglycans (GAGs) (1 - 3). These type 1 transmembrane proteins show conserved cytoplasmic domains and divergent extracellular domains (1 - 3). Human Syndecan-3 is synthesized as a 442 amino acid (aa) core protein with a 44 aa signal sequence, a 343 aa extracellular domain (ECD), a 21 aa transmembrane (TM) region and a 34 aa cytoplasmic tail with a binding site for PDZ domains (1). The ECD of human Syndecan-3 shares 83%, 83%, 92%, 91% and 91% aa identity with of mouse, rat, equine, bovine and canine Syndecan-3, respectively. Splice isoforms of 384 aa and 346 aa, containing either a 28 aa substitution for aa 1 - 86 or deletion of aa 1 - 96, have been reported (4). Syndecan-3 contains four conserved closely-spaced GAG attachment sites near the N-terminus and unique threonine-rich and mucin-like sequences near the membrane (4). Addition of glycan side chains results in an apparent size of 120 - 150 kDa. Non-covalent homodimerization of Syndecan-3 or, potentially, heterodimerization with Syndecan-2 or -4, is dependent on the transmembrane domain (5). A cleavage site near the TM domain allows shedding of soluble ECD; the remainder of the molecule undergoes regulated intramembrane proteolysis (6). Syndecan-3 is expressed in the nervous system and at limb buds during development (1, 2). It is expressed on neuronal axons and Schwann cell perinodal processes, promoting nerve cell migration and synapse formation (7, 8). Roles in memory and body weight regulation have been described (2, 9, 10). Through localization of growth factors such as FGF2, HGF and TGF-beta, it regulates expression of molecules important for differentiation of muscle and bone, such as myogenin, BMP-2 and hedgehog family members (1, 2, 11 - 13). In adults, it is upregulated during regeneration, such as following myocardial infarction (14).

  1. Tkachenko, E. et al. (2005) Circ. Res. 96:488.
  2. Reizes, O. et al. (2008) Int. J. Biochem. Cell Biol. 40:28.
  3. Carey, D.J. et al. (1997) J. Biol. Chem. 272:2873.
  4. ENTREZ protein Accession # O75056, EAX076736 and EAX07637.
  5. Dews, I.C. and K.R. MacKenzie (2007) Proc. Natl. Acad. Sci. USA 104:20782.
  6. Schultz, J.G. et al. (2003) J. Biol. Chem. 278:48651.
  7. Hienola, A. et al. (2006) J. Cell Biol. 174:569.
  8. Goutebroze, L. et al. (2003) BMC Neurosci. 4:29.
  9. Kaksonen, M. et al. (2002) Mol. Cell. Neurosci. 21:158.
  10. Strader. A.D. et al. (2004) J. Clin. Invest. 114:1354.
  11. Cornelison, D.D.W. et al. (2004) Genes Dev. 18:2231.
  12. Fisher, M.C. et al. (2006) Matrix Biol. 25:27.
  13. Pacifici, M. et al. (2005) J. Bone Miner. Metab. 23:191.
  14. Finsen, A.V. et al. (2004) Physiol. Genomics 16:301.
Entrez Gene IDs
9672 (Human); 20970 (Mouse)
Alternate Names
KIAA0468; N-Syndecan; SDC3; SDCN; SYND3syndecan proteoglycan 3; syndecan 3 (N-syndecan); syndecan 3; syndecan neural type; Syndecan3; Syndecan-3

Citation for Recombinant Human Syndecan-3 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Soluble syndecan-3 binds chemokines, reduces leukocyte migration in vitro and ameliorates disease severity in models of rheumatoid arthritis
    Authors: AD Eustace, EF McNaughton, S King, O Kehoe, A Kungl, D Mattey, AH Nobbs, N Williams, J Middleton
    Arthritis Res. Ther., 2019;21(1):172.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo


No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Natural Proteins

Reconstitution Buffers

Reviews for Recombinant Human Syndecan-3 Protein, CF

Average Rating: 5 (Based on 1 Review)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used Recombinant Human Syndecan-3 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

Recombinant Human Syndecan-3 Protein, CF
By Anonymous on 09/14/2017
Application: SDS-PAGE Control

Visualized using Simply Blue Safe Stain.