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Recombinant Human TGF-beta 3 Protein, CF

Name: Recombinant Human TGF-beta 3 Protein, CF
Brand: R&D Systems
SKU: BT-TGFB3
Price: 1376 USD
Availability: InStock

R&D Systems | Catalog # BT-TGFB3

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Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Source

E. coli

Accession Number

P10600.1

Applications

Bioactivity

View all TGF-beta 3 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human TGF-beta 3 protein
Ala301-Ser412

Purity

>97%, by SDS-PAGE with quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala301

Predicted Molecular Mass

13 kDa

SDS-PAGE

10 kDa, under reducing conditions.

Activity

Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389.
The ED₅₀ for this effect is 7.50-75.0 pg/mL.

Scientific Data Images for Recombinant Human TGF-beta 3 Protein, CF

Equivalent Bioactivity of GMP,Animal-Free, and RUO gradesof Recombinant Human TGF-beta 3.

Equivalent bioactivity of GMP (BT-TGFB3-GMP), Animal-Free (BT-TGFB3-AFL) and RUO(Catalog # BT-TGFB3) grades of Recombinant Human TGF-beta 3 as measured by its ability to inhibit the IL-4-dependent proliferation ofHT‑2 mouse T cells (orange, green, red, respectively).

Recombinant Human TGF-beta 3 Protein Bioactivity.

Recombinant Human TGF-beta 3Protein (Catalog # BT-TGFB3)inhibits recombinant mouse IL-4induced proliferation in the HT-2mouse T cell line. The ED₅₀ forthis effect is 7.50-75.0 pg/mL.

Recombinant Human TGF-beta 3 Protein SDS-PAGE.

2 μg/lane of Recombinant Human TGF-beta 3 Protein (Catalog # BT-TGFB3) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 10 kDa, under reducing conditions.

Formulation, Preparation, and Storage

BT-TGFB3

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with Trehalose.
Reconstitution	Reconstitute the 20 μg size at 100 μg/mL in 4mM HCl. Reconstitute all other sizes at 500 μg/mL in 4mM HCl.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: TGF-beta 3

TGF beta 3 (transforming growth factor-beta 3) is a member of a TGF-beta superfamily subgroup that is defined by their structural and functional similarities (1-5). TGF-beta 3 and its closely related proteins, TGF-beta 1 and -beta 2, act as cellular switches to regulate immune function, cell proliferation, and epithelial-mesenchymal transition (4, 6, 7). The non-redundant biological effects of TGF-beta 3 include involvement in palatogenesis, chondrogenesis, and pulmonary development (1, 2, 7-9). Human TGF- beta 3 cDNA encodes a 412 amino acid (aa) precursor that contains a 20 aa signal peptide and a 392 aa proprotein. The proprotein is processed by a furinlike convertase to generate a 220 aa latencyassociated peptide (LAP) and a 112 aa mature TGF-beta 3 (10, 11). Mature human TGF-beta 3 shows 100%, 99%, and 98% aa identity with mouse/dog/horse, rat, and pig TGF- beta 3, respectively. TGF-beta 3 is secreted as a complex with LAP. This latent form of TGF-beta 3 becomes active upon cleavage by plasmin, matrix metalloproteases, thrombospondin-1, and a subset of integrins (12). TGF-beta 3 binds with high affinity to TGF-beta RII, a type II serine/threonine kinase receptor. This receptor then phosphorylates and activates type I serine/threonine kinase receptors, TGF- beta RI or ALK-1, to modulate transcription through Smad phosphorylation (13-15). The divergent biological effects exerted by individual TGF-beta isoforms is dependent upon the recruitment of co-receptors (TGF- beta RIII and endoglin) and the subsequent initiation of Smad-dependent or -independent signaling pathways (14, 16, 17).

References

Barrio, M.C. et al. (2014) Cells Tissues Organs. [Epub ahead of print; PMID 24861080].
Doetschman, T. et al. (2012) Genesis 50:59.
Mittl, P.R. et al. (1996) Protein Sci. 5:1261.
Sporn, M.B. (2006) Cytokine Growth Factor Rev. 17:3.
Wahl, S.M. et al. (2006) Immunol. Rev. 213:213.
Chang, H. et al. (2002) Endocr. Rev. 23:787.
Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
Jin, J.Z. et al. (2014) Dev. Dyn. [Epub ahead of print; PMID 25104574].
Tang, Q.O. et al. (2009) Expert Opin. Biol Ther. 9:689.
Derynck, R. et al. (1988) EMBO J. 7:3737.
Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
Oklu, R. and R. Hesketh (2000) Biochem. J. 352 Pt 3:601.
Cui, X.M. and C.F. Shuler (2000) Int. J. Dev. Biol. 44:397.
de Caestecker, M. (2004) Cytokine Growth Factor Rev. 15:1.
Nakajima, A. et al. (2007) Dev. Dyn. 236:791.
Iwata, J. et al. (2012) J. Clin. Invest. 122:873.
Gatza, C.E. et al. (2010) Cell. Signal. 22:1163.