Recombinant Human Thrombopoietin R Protein, CF Summary
Ser25-Trp491, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 250 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: Thrombopoietin R/Tpo R
Thrombopoietin receptor (Tpo R), also known as myeloproliferative leukemia protein (c-mpl), is a 95 kDa type I transmembrane protein that is a member of the type I cytokine receptor family within the hematopoietin/cytokine receptor superfamily (1 - 4). The 635 amino acid (aa) full-length human Tpo R contains a 25 aa signal sequence, a 466 aa extracellular domain with a ligand binding domain and two fibronectin type III domains, a transmembrane (TM) domain and a cytoplasmic domain. The extracellular domain of human Tpo R shares 78%, 76%, 81%, 82% and 80% aa identity with mouse, rat, bovine, canine and equine Tpo R, respectively. Humans produce three distinct mRNA species; a P-form, a K-form, and a truncated form (Mpl-tr) lacking a TM domain (3 - 7). The P-form encodes the full-length receptor. The Mpl-tr form, which is expressed in both human and mouse, is intracellular and targets the P-form for degradation (5, 6). The 579 aa K-form has an alternate cytoplasmic domain, but does not dimerize with, or inhibit, the P-form (7). Thrombopoietin (Tpo) is a key regulator of megakaryocytopoiesis, thrombopoiesis and hematopoietic stem cell self-renewal, as reflected by expression of the Tpo R on megakaryocytes, platelets and hematopoietic progenitors (2, 8). Receptor dimerization occurs upon Tpo binding and initiates signaling through the Ras/MAP and JAK/STAT pathways (1, 2). Internalization and degradation of Tpo following Tpo R binding serves to downregulate circulating Tpo (9). Tpo R expressed at low levels on endothelial cells does not appear to contribute to regulation of Tpo (10). Inactivating mutations of Tpo R cause thrombocytopenia, and absence of functional Tpo R is lethal in humans, but not mice. Other mutations, including an activating change in the TM domain, can cause thrombocytosis (11, 12).
- Kaushansky, K. (2005) J. Clin. Invest. 115:3339.
- Deutsch, V.R. and A. Tomer (2006) Br. J. Haematol. 134:453.
- Vigon, I. et al. (1992) Proc. Natl. Acad. Sci. USA 89:5640.
- Mignotte, V. et al. (1994) Genomics 20:5.
- Li, J. et al. (2000) Cytokine 12:835.
- Coers, J. et al. (2004) J. Biol. Chem. 279:36397.
- Millot, G.A. et al. (2002) Exp. Hematol. 30:166.
- Tong, W. et al. (2007) Exp. Hematol. July 14 [Epub ahead of print].
- Li, J. et al. (1999) Br. J. Haematol. 106:345.
- Geddis, A.E. et al. (2006) Exp. Hematol. 34:82.
- Germeshausen, M. et al. (2006) Hum. Mutat. 27:296.
- Ding, J. et al. (2004) Blood 103:4198.
Citations for Recombinant Human Thrombopoietin R Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Affinity maturation of an TpoR targeting antibody in full-length IgG form for enhanced agonist activity
Authors: Z Yang, M Du, W Wang, X Xin, P Ma, H Zhang, RA Lerner
Protein Eng. Des. Sel., 2018;0(0):.
Species: Transformed Yeast
Sample Types: Whole Cells
Applications: Flow Cytometry
Thrombopoietin contributes to enhanced platelet activation in cigarette smokers.
Authors: Lupia E, Bosco O, Goffi A
Sample Types: Plasma
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