Recombinant Human TIM-1/KIM-1/HAVCR Fc Chimera Protein, CF

  
  • Purity
    >85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The ED50 for this effect is 0.6-3.6 μg/mL.
  • Source
    Human embryonic kidney cell, HEK293-derived
    Human Tim-1/Kim-1/HAVCR
    (Ser21-Thr288)
    Accession # AAC39862
    IEGRMD Human IgG1
    (Pro100-Lys330)
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Ser21
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    55 kDa
  • SDS-PAGE
    112-129 kDa, reducing conditions
9319-TM
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human TIM‑1/KIM‑1/HAVCR Fc Chimera (Catalog #9319-TM) inhibits anti-CD3-induced proliferation of PHA-activated human Tcells. The ED50 for this effect is 0.6-3.6 μg/mL.
Background: TIM-1/KIM-1/HAVCR
T cell immunoglobulin and mucin domain 1 (TIM-1), also known as KIM-1 and HAVcr1, is a member of the TIM family which is involved in the regulation of innate and adaptive immune responses (1). TIM-1 is a type I transmembrane protein that contains an N-terminal immunoglobulin-like domain, a mucin domain with O- and N-linked carbohydrates, a transmembrane segment, and a cytoplasmic signaling domain (2). Multiple TIM-1 variants can be produced due to polymorphisms or alternative splicing resulting in deletions in the mucin domain. Within the extracellular domain, human TIM-1 shares 41% aa sequence identity with mouse and rat TIM-1. TIM-1 is expressed on splenic B cells, IL-10+ regulatory B cells, CD4+ T cells, mast cells, invariant NKT (iNKT) cells, dendritic cells, kidney epithelium and a broad range of mucosal epithelium (1, 3-5). It is upregulated on activated Th2 cells, after dendritic cell maturation, and on kidney tubular epithelial cells after injury (6-9). Metalloproteinase-mediated cleavage of TIM-1 at the membrane-proximal region results in the release of a soluble form of TIM-1 which is detectable in the urine and in circulation (10). TIM-1 serves as a receptor for phosphatidylserine, LMIR5/CD300b, TIM-1 (homophilic), TIM-4, IgA, and the glycoproteins of a number of enveloped viruses (2, 11-16). Its interaction with phosphatidylserine enables TIM-1 to mediate the phagocytosis of apoptotic cells (12, 13) and iNKT cell activation (17). TIM-1 binding induces the activation of LMIR5-expressing myeloid cells, contributing to tissue homeostasis as well as damage following kidney injury (14). TIM-1 ligation co-stimulates T cell activation and enhances Th2 cytokine production (7, 15). In humans, TIM-1 serves as a cellular entry receptor for various viruses, including hepatitis A virus, Ebolavirus and Marburgvirus (2, 11).
  • References:
    1. Du, P. et al. (2016) J. Immunol. Res. 2016:8605134.
    2. Feigelstock, D. et al. (1998) J. Virol. 72:6621.
    3. Ding, Q. et al. (2011) J. Clin. Invest. 121:3645.
    4. Ma, J. et al. (2011) Biochem. Biophys. Res. Commun. 406:223.
    5. de Souza, A.J. et al. (2005) Proc. Natl. Acad. Sci. USA 102:17113.
    6. Kuehn, E.W. et al. (2002) Am. J. Physiol. Renal Physiol. 283:F1326.
    7. Umetsu, S.E. et al. (2005) Nat. Immunol. 6:447.
    8. Xiao, S. et al. (2011) Eur. J. Immunol. 41:1539.
    9. Ichimura, T. et al. (1998) J. Biol. Chem. 273:4135.
    10. Bailly, V. et al. (2002) J. Biol. Chem. 277:39739.
    11. Kondratowicz, A.S. et al. (2011) Proc. Natl. Acad. Sci. USA 108:8426.
    12. Miyanishi, M. et al. (2007) Nature 450:435.
    13. Kobayashi, N. et al. (2007) Immunity 27:927.
    14. Yamanishi, Y. et al. (2010) J. Exp. Med. 207:1501.
    15. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
    16. Tami, C. et al. (2007) J. Virol. 81:3437.
    17. Lee, H.H. et al. (2010) J. Immunol. 185:5225.
  • Long Name:
    T Cell Immunoglobulin Mucin-1
  • Entrez Gene IDs:
    26762 (Human); 171283 (Mouse); 286934 (Rat); 102141332 (Cynomolgus Monkey)
  • Alternate Names:
    CD365; HAVCR1; HAVCR-1; HAVCRT cell immunoglobin domain and mucin domain protein 1; hepatitis A virus cellular receptor 1; Kidney injury molecule 1; KIM1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIM1; TIM-1; TIM-1TIM; TIM1TIMD-1; TIMD1T-cell membrane protein 1
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