Recombinant Human TIM-4 Fc Chimera Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The ED50 for this effect is 0.15-0.75 μg/mL.
  • Source
    Human embryonic kidney cell, HEK293-derived
    Human TIM-4
    (Glu25-Gln314)
    Accession # Q96H15
    IEGRMD Human IgG1
    (Pro100-Lys330)
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Glu25
  • Predicted Molecular Mass
    58 kDa
  • SDS-PAGE
    95-113 kDa, reducing conditions
9300-TM
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human TIM-4 Fc Chimera (Catalog #
9300-TM)inhibits anti-CD3-induced proliferation of human T cells. The ED50 for thiseffect is 0.15-0.75 μg/mL.
Background: TIM-4
TIM-4 (T cell; immunoglobulin; mucin-4), also known as SMUCKLER,  is a 60 kDa member of the TIM family of immune regulating proteins. TIMs are type I transmembrane proteins with one Ig-like V domain and one Ser/Thr-rich mucin domain (1). Mature human TIM-4 consists of a 290 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 43 aa cytoplasmic tail (2). Structurally, TIM-4 is distinguished from other TIMs by the presence of an RGD motif in its Ig domain and the lack of a site for tyrosine phosphorylation in its cytoplasmic tail. Within the ECD human TIM-4 shares 45% aa sequence identity with mouse and rat TIM-4. TIM-4 is expressed by macrophages and mature dendritic cells but not by lymphocytes (2, 3). It binds specifically to TIM-1 which is also the cellular receptor for hepatitis A virus and has been implicated in the development of asthma (3-5). Among hematopoietic cells, TIM-1 is expressed on activated B and T cells, preferentially in the Th2 subset of CD4+ T cells (3, 6). The interaction of TIM-4 with TIM-1 induces co-stimulatory and hyperproliferative signals in T cells (3). TIM-4 also binds to phosphatidylserine (PS) on the surface of apoptotic cells (7). It enhances the clearance of apoptotic cells including T cells in the contraction phase of immune responses, apoptotic macrophages that accumulate in atherosclerosis, and dying neurons in the brain (7-10). TIM-4 is upregulated on some tumor cells and tumor-associated myeloid cells where it binds Integrin alpha V beta 3, contributes to tumor progression, and inhibits tumor-specific cellular immunity (11, 12).
  • References:
    1. Fang, X.Y. et al. (2015) Autoimmunity 48:189.
    2. Shakhov, A.N. et al. (2004) Eur. J. Immunol. 34:494.
    3. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
    4. Feigelstock, D. et al. (1998) J. Virol. 72:6621.
    5. McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
    6. Khademi, M. et al. (2004) J. Immunol. 172:7169.
    7. Kobayashi, N. et al. (2007) Immunity 27:927.
    8. Albacker, L.A. et al. (2010) J. Immunol. 185:6839.
    9. Mazaheri, F. et al. (2014) Nat. Commun. 5:4046.
    10. Foks, A.C. et al. (2016) Arterioscler. Thromb. Vasc. Biol. 36:456.
    11. Zhang, Q. et al. (2015) Br. J. Cancer 113:1484.
    12. Baghdadi, M. et al. (2013) Immunity 39:1070.
  • Long Name:
    T Cell Immunoglobulin Mucin-4
  • Entrez Gene IDs:
    91937 (Human); 276891 (Mouse); 102140672 (Cynomolgus Monkey)
  • Alternate Names:
    FLJ27515; Smuckler; T-cell immunoglobulin and mucin domain containing 4; T-cell immunoglobulin and mucin domain-containing protein 4; T-cell membrane protein 4; TIM4; TIM-4; TIMD4; TIMD-4
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