Recombinant Human TIM-4 Fc Chimera Protein, CF

Datasheet / CoA / SDS
Product Details
Supplemental Products
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The ED50 for this effect is 0.15-0.75 μg/mL.
  • Source
    Human embryonic kidney cell, HEK293-derived human TIM-4 protein
    Human TIM-4
    Accession # Q96H15
    IEGRMD Human IgG1
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
  • Predicted Molecular Mass
    58 kDa
    95-113 kDa, reducing conditions
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Image
Recombinant Human TIM-4 Fc Chimera (Catalog #
9300-TM)inhibits anti-CD3-induced proliferation of human T cells. The ED50 for thiseffect is 0.15-0.75 μg/mL.
Background: TIM-4
TIM-4 (T cell; immunoglobulin; mucin-4), also known as SMUCKLER,  is a 60 kDa member of the TIM family of immune regulating proteins. TIMs are type I transmembrane proteins with one Ig-like V domain and one Ser/Thr-rich mucin domain (1). Mature human TIM-4 consists of a 290 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 43 aa cytoplasmic tail (2). Structurally, TIM-4 is distinguished from other TIMs by the presence of an RGD motif in its Ig domain and the lack of a site for tyrosine phosphorylation in its cytoplasmic tail. Within the ECD human TIM-4 shares 45% aa sequence identity with mouse and rat TIM-4. TIM-4 is expressed by macrophages and mature dendritic cells but not by lymphocytes (2, 3). It binds specifically to TIM-1 which is also the cellular receptor for hepatitis A virus and has been implicated in the development of asthma (3-5). Among hematopoietic cells, TIM-1 is expressed on activated B and T cells, preferentially in the Th2 subset of CD4+ T cells (3, 6). The interaction of TIM-4 with TIM-1 induces co-stimulatory and hyperproliferative signals in T cells (3). TIM-4 also binds to phosphatidylserine (PS) on the surface of apoptotic cells (7). It enhances the clearance of apoptotic cells including T cells in the contraction phase of immune responses, apoptotic macrophages that accumulate in atherosclerosis, and dying neurons in the brain (7-10). TIM-4 is upregulated on some tumor cells and tumor-associated myeloid cells where it binds Integrin alpha V beta 3, contributes to tumor progression, and inhibits tumor-specific cellular immunity (11, 12).
  • References:
    1. Fang, X.Y. et al. (2015) Autoimmunity 48:189.
    2. Shakhov, A.N. et al. (2004) Eur. J. Immunol. 34:494.
    3. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
    4. Feigelstock, D. et al. (1998) J. Virol. 72:6621.
    5. McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
    6. Khademi, M. et al. (2004) J. Immunol. 172:7169.
    7. Kobayashi, N. et al. (2007) Immunity 27:927.
    8. Albacker, L.A. et al. (2010) J. Immunol. 185:6839.
    9. Mazaheri, F. et al. (2014) Nat. Commun. 5:4046.
    10. Foks, A.C. et al. (2016) Arterioscler. Thromb. Vasc. Biol. 36:456.
    11. Zhang, Q. et al. (2015) Br. J. Cancer 113:1484.
    12. Baghdadi, M. et al. (2013) Immunity 39:1070.
  • Long Name:
    T Cell Immunoglobulin Mucin-4
  • Entrez Gene IDs:
    91937 (Human); 276891 (Mouse); 102140672 (Cynomolgus Monkey)
  • Alternate Names:
    FLJ27515; Smuckler; T-cell immunoglobulin and mucin domain containing 4; T-cell immunoglobulin and mucin domain-containing protein 4; T-cell membrane protein 4; TIM4; TIM-4; TIMD4; TIMD-4
Related Research Areas


No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs
Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 1 (PBS)

View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Recombinant Human TIM-4 Fc Chimera Protein, CF?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Us to Order


Customer Information
You May Also Like

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

R&D Systems Guarantee