Recombinant Human UbcH7/UBE2L3 Dominant Negative, CF
Recombinant Human UbcH7/UBE2L3 Dominant Negative, CF Summary
Product Specifications
Accession # P68036
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E2-642
| Formulation | Supplied as a solution in HEPES, NaCl, DTT and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: UbcH7/UBE2L3
Ubiquitin-conjugating Enzyme H7 (UbcH7), also known as Ubiquitin-conjugating Enzyme E2L 3 (UBE2L3), is a member of the Ubiquitin-conjugating (E2) enzyme family (1). It has a predicted molecular weight of approximately 18 kDa. The human UbcH7 protein shares 100% amino acid (aa) sequence identity with the mouse and rat orthologs. UbcH7 has an E2 catalytic core domain that contains an active site cysteine residue and comprises 152 of its 154 aa residues. UbcH7 is catalytically active with HECT and RBR domain-containing families of Ubiquitin ligases (E3s) (2,3). UbcH7 localizes to both the nucleus and cytoplasm in human cells. In mice, its ortholog is expressed in many tissues including brain, muscle, heart, lung, lymph node, spleen, thymus, and testis (4,5). UbcH7 depletion results in an extended S phase and a reduced rate of proliferation, suggesting that it may play a role in the cell cycle (6). In humans, single nucleotide polymorphisms in UbcH7 are associated with systemic lupus erythematosus and Crohn's disease, suggesting that UbcH7 is important for proper immune system function (7,8). This enzyme has a mutation of the active site from cysteine to serine which abolishes the ability of UbcH7 to transfer ubiquitin to an accepting E3 protein. This product is ideal for use as a negative control, competitive control, or to study protein-protein interactions.
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- Wenzel, D.M. et al. (2011) Nature 474:105.
- Garside, H. et al. (2006) J. Endocrinol. 190:621.
- Harbers, K. et al. (1996) Proc. Natl. Acad. Sci. USA 93:12412.
- Whitcomb, E.A. et al. (2009) Mol. Biol. Cell 20:1.
- Wang, S. et al. (2012) Genes Immun. 13:380.
- Fransen, K. et al. (2010) Hum. Mol. Genet. 19:3482.
Citation for Recombinant Human UbcH7/UBE2L3 Dominant Negative, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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An engineered platform for reconstituting functional multisubunit SCF E3 ligase in vitro
Authors: H Liu, S Liu, H Yu, X Huang, Y Wang, L Jiang, X Meng, G Liu, M Chen, Y Jing, F Yu, B Wang, J Li
Molecular plant, 2022-06-24;0(0):.
Species: Human
Sample Types: Protein
Applications: Bioassay
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