Recombinant Human Ubiquilin 1 Tandem UBA (TUBE2) Agarose, CF

Catalog # Availability Size / Price Qty
AM-130-250
Product Details
Citations (4)
FAQs
Reviews (1)

Recombinant Human Ubiquilin 1 Tandem UBA (TUBE2) Agarose, CF Summary

Ubiquilin 1 is a ubiquitously expressed member of the UBL-UBA family of Ubiquitin-binding proteins that is 589 amino acids (aa) in length and has a predicted molecular weight of 62.5 kDa (1). Human Ubiquilin 1 shares 88% aa sequence identity with the mouse and rat orthologs. It contains an N-terminal Ubiquitin-like (UBL) domain and a C-terminal Ubiquitin-associated (UBA) domain (2). The UBA domain is able to interact with mono- and poly-Ubiquitin chains, with a similar affinity for Lys48- and Lys63-linked chains (3,4). In contrast, the UBL domain interacts with Ubiquitin-interacting motif (UIM)-containing proteins, such as S5a/Angiocidin, a 19S Proteasome component (5). It is thought that Ubiquilin 1 may act as a molecular shuttle, bringing poly-ubiquitinated proteins to the 26S Proteasome by simultaneously binding poly-Ubiquitin via its UBA domain and the 19S Proteasome via its UBL domain (5). Ubiquilin 1 also binds two endoplasmic reticulum (ER)-localized proteins, Herp and Erasin, and may function in the ER-associated degradation pathway (6,7). It has been reported to co-precipitate with LC3/MAP1LC3A/Apg8p3, an autophagosomal marker, suggesting that it may play a role in autophagy (8,9). Single nucleotide polymorphisms in the UBQLN1 gene have been linked to Alzheimer's disease (AD) (10). Furthermore, Ubiquilin 1 may regulate the processing of APP, which is associated with AD (11).

 

Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitinated proteins. TUBEs display increased affinity for poly-Ubiquitin moieties over the single Ubiquitin binding associated domain (UBA). TUBEs also display a protective effect on poly-ubiquitinated proteins, allowing for detection at relatively low abundance. This affinity resin can be used for the enrichment, isolation and identification of K48-linked (preferentially) or K63-linked poly-Ubiquitin chains or ubiquitinated substrates that contain these linkages.

Specifications

Source
E. coli
Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Do not freeze.Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 3 months from date of receipt, 2 to 8 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
Applications
Enzyme Activity
Species
Human

Product Datasheets

Background: Ubiquilin 1

References
  1. Lee, D.Y. & E.J. Brown (2012) Biol. Chem. 393:441.
  2. Mah, A.L. et al. (2000) J. Cell Biol. 151:847.
  3. Raasi, S. et al. (2005) Nat. Struct. Mol. Biol. 12:708.
  4. Zhang, D. et al.(2008) J. Mol. Biol. 377:162.
  5. Ko, H.S. et al. (2004) FEBS Lett. 566:110.
  6. Kim, T.Y. et al. (2008) Biochem. Biophys. Res. Commun. 369:741.
  7. Lim, P.J. et al. (2009) J. Cell Biol. 187:201.
  8. N'Diaye, E.N. et al. (2009) EMBO Rep. 10:173.
  9. Rothenberg, C. et al. (2010) Hum. Mol. Genet. 19:3219.
  10. Bertram, L. et al. (2005) N. Engl. J. Med. 352:884.
  11. El Ayadi, A. et al. (2012) Proc. Natl. Acad. Sci. USA 109:13416.
Entrez Gene IDs
29979 (Human); 56085 (Mouse); 114590 (Rat)
Alternate Names
DA41; DA41FLJ90054; DSK2; hPLIC-1; PLIC1; PLIC-1; PLIC-1UBQN; Protein linking IAP with cytoskeleton 1; Ubiquilin 1; ubiquilin-1; UBQLN1; UBQN; XDRP1

Citations for Recombinant Human Ubiquilin 1 Tandem UBA (TUBE2) Agarose, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. DNA damage and transcription stress cause ATP-mediated redesign of metabolism and potentiation of anti-oxidant buffering
    Authors: C Milanese, CR Bombardier, S Sepe, S Barnhoorn, C Payán-Gomé, D Caruso, M Audano, S Pedretti, WP Vermeij, RMC Brandt, A Gyenis, MM Wamelink, AS de Wit, RC Janssens, R Leen, ABP van Kuilen, N Mitro, JHJ Hoeijmaker, PG Mastrobera
    Nat Commun, 2019;10(1):4887.  2019
  2. Vms1 and ANKZF1 peptidyl-tRNA hydrolases release nascent chains from stalled ribosomes
    Authors: R Verma, KM Reichermei, AM Burroughs, RS Oania, JM Reitsma, L Aravind, RJ Deshaies
    Nature, 2018;0(0):.  2018
  3. p97/VCP promotes degradation of CRBN substrate glutamine synthetase and neosubstrates
    Authors: TV Nguyen, J Li, CJ Lu, JL Mamrosh, G Lu, BE Cathers, RJ Deshaies
    Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.  2017
  4. Capzimin is a potent and specific inhibitor of proteasome isopeptidase Rpn11
    Authors: J Li, T Yakushi, F Parlati, AL Mackinnon, C Perez, Y Ma, KP Carter, S Colayco, G Magnuson, B Brown, K Nguyen, S Vasile, E Suyama, LH Smith, E Sergienko, AB Pinkerton, TD Chung, AE Palmer, I Pass, S Hess, SM Cohen, RJ Deshaies
    Nat. Chem. Biol, 2017;0(0):.  2017

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Recombinant Human Ubiquilin 1 Tandem UBA (TUBE2) Agarose, CF
By Anonymous on 08/02/2018
Application:

Worked perfectly well for my project with C. elegans.