Recombinant Human Ubiquilin 1 Tandem UBA (TUBE2) Agarose, CF
Recombinant Human Ubiquilin 1 Tandem UBA (TUBE2) Agarose, CF Summary
Ubiquilin 1 is a ubiquitously expressed member of the UBL-UBA family of Ubiquitin-binding proteins that is 589 amino acids (aa) in length and has a predicted molecular weight of 62.5 kDa (1). Human Ubiquilin 1 shares 88% aa sequence identity with the mouse and rat orthologs. It contains an N-terminal Ubiquitin-like (UBL) domain and a C-terminal Ubiquitin-associated (UBA) domain (2). The UBA domain is able to interact with mono- and poly-Ubiquitin chains, with a similar affinity for Lys48- and Lys63-linked chains (3,4). In contrast, the UBL domain interacts with Ubiquitin-interacting motif (UIM)-containing proteins, such as S5a/Angiocidin, a 19S Proteasome component (5). It is thought that Ubiquilin 1 may act as a molecular shuttle, bringing poly-ubiquitinated proteins to the 26S Proteasome by simultaneously binding poly-Ubiquitin via its UBA domain and the 19S Proteasome via its UBL domain (5). Ubiquilin 1 also binds two endoplasmic reticulum (ER)-localized proteins, Herp and Erasin, and may function in the ER-associated degradation pathway (6,7). It has been reported to co-precipitate with LC3/MAP1LC3A/Apg8p3, an autophagosomal marker, suggesting that it may play a role in autophagy (8,9). Single nucleotide polymorphisms in the UBQLN1 gene have been linked to Alzheimer's disease (AD) (10). Furthermore, Ubiquilin 1 may regulate the processing of APP, which is associated with AD (11).
Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitinated proteins. TUBEs display increased affinity for poly-Ubiquitin moieties over the single Ubiquitin binding associated domain (UBA). TUBEs also display a protective effect on poly-ubiquitinated proteins, allowing for detection at relatively low abundance. This affinity resin can be used for the enrichment, isolation and identification of K48-linked (preferentially) or K63-linked poly-Ubiquitin chains or ubiquitinated substrates that contain these linkages.
- 3 months from date of receipt, 2 to 8 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after opening.
Background: Ubiquilin 1
- Lee, D.Y. & E.J. Brown (2012) Biol. Chem. 393:441.
- Mah, A.L. et al. (2000) J. Cell Biol. 151:847.
- Raasi, S. et al. (2005) Nat. Struct. Mol. Biol. 12:708.
- Zhang, D. et al.(2008) J. Mol. Biol. 377:162.
- Ko, H.S. et al. (2004) FEBS Lett. 566:110.
- Kim, T.Y. et al. (2008) Biochem. Biophys. Res. Commun. 369:741.
- Lim, P.J. et al. (2009) J. Cell Biol. 187:201.
- N'Diaye, E.N. et al. (2009) EMBO Rep. 10:173.
- Rothenberg, C. et al. (2010) Hum. Mol. Genet. 19:3219.
- Bertram, L. et al. (2005) N. Engl. J. Med. 352:884.
- El Ayadi, A. et al. (2012) Proc. Natl. Acad. Sci. USA 109:13416.
Citations for Recombinant Human Ubiquilin 1 Tandem UBA (TUBE2) Agarose, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
Filter your results:
DNA damage and transcription stress cause ATP-mediated redesign of metabolism and potentiation of anti-oxidant buffering
Authors: C Milanese, CR Bombardier, S Sepe, S Barnhoorn, C Payán-Gomé, D Caruso, M Audano, S Pedretti, WP Vermeij, RMC Brandt, A Gyenis, MM Wamelink, AS de Wit, RC Janssens, R Leen, ABP van Kuilen, N Mitro, JHJ Hoeijmaker, PG Mastrobera
Nat Commun, 2019;10(1):4887. 2019
Vms1 and ANKZF1 peptidyl-tRNA hydrolases release nascent chains from stalled ribosomes
Authors: R Verma, KM Reichermei, AM Burroughs, RS Oania, JM Reitsma, L Aravind, RJ Deshaies
Nature, 2018;0(0):. 2018
p97/VCP promotes degradation of CRBN substrate glutamine synthetase and neosubstrates
Authors: TV Nguyen, J Li, CJ Lu, JL Mamrosh, G Lu, BE Cathers, RJ Deshaies
Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):. 2017
Capzimin is a potent and specific inhibitor of proteasome isopeptidase Rpn11
Authors: J Li, T Yakushi, F Parlati, AL Mackinnon, C Perez, Y Ma, KP Carter, S Colayco, G Magnuson, B Brown, K Nguyen, S Vasile, E Suyama, LH Smith, E Sergienko, AB Pinkerton, TD Chung, AE Palmer, I Pass, S Hess, SM Cohen, RJ Deshaies
Nat. Chem. Biol, 2017;0(0):. 2017
No product specific FAQs exist for this product, however you mayView all Proteins and Enzyme FAQs
Reviews for Recombinant Human Ubiquilin 1 Tandem UBA (TUBE2) Agarose, CF
Average Rating: 5 (Based on 1 Review)
Have you used Recombinant Human Ubiquilin 1 Tandem UBA (TUBE2) Agarose, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image