Recombinant Human UFM1 Agarose Protein, CF

Catalog # Availability Size / Price Qty
UL-530-500
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (2)
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Recombinant Human UFM1 Agarose Protein, CF Summary

Product Specifications

Activity
UFM1 Agarose is ideal for the enrichment of known UFM1-interacting proteins as well as the discovery of novel UFM1-interacting proteins. We recommend equilibrating the resin by washing with 5-10 mL of your desired aqueous buffer.
Source
E. coli-derived human UFM1 protein
Accession #

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

UL-530

Formulation

0.5 ml Ufm1 agarose is supplied in a 1 ml total volume of 50 mM Hepes pH 7.5, 250 mM NaCl.

Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Do not freeze.
  • 3 months from date of receipt, 2 to 8 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
Reconstitution Calculator

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Background: UFM1

Human Ubiquitin-fold Modifier 1 (UFM1), also known as BM-002, is an 85 amino acid (aa) member of the Ubiquitin-like protein family that has a predicted molecular weight of 9.1 kDa. Human and mouse UFM1 share 100% aa sequence identity and are primarily localized in the nucleus, but can also be detected in the cytoplasm and the endoplasmic reticulum (ER) (1). ER localization of UFM1 appears to be dependent on the co-expression of UFBP1 (2). Initially expressed as an inactive precursor, UFM1 undergoes proteolytic cleavage at the C-terminus to expose a conserved glycine residue that is necessary for UFM1 conjugation to target proteins. Conjugation of UFM1 to target proteins requires a UFM1-activating (E1) enzyme, a UFM1-conjugating (E2) enzyme, and a UFM1 ligase (E3) (1,3). UFM1 has been shown to be a mediator of ER stress-induced apoptosis and to promote the pathogenesis of Leishmania (2,4). This protein is covalently coupled to agarose beads via primary amines allowing for a fully functional C-terminus. Useful for isolation and capture of UFM1 interacting proteins and/or enzymes that have an affinity for this Ubiquitin-like protein.

References
  1. Komatsu, M. et al. (2004) EMBO J. 23:1977.
  2. Lemaire, K. et al. (2011) PLoS One 6:e18517.
  3. Tatsumi, K. et al. (2010) J. Biol. Chem. 285:5417.
  4. Gannavaram, S. et al. (2012) Mol. Microbiol. 86:187.
 
Long Name
Ubiquitin-fold Modifier 1
Entrez Gene IDs
51569 (Human); 67890 (Mouse); 365797 (Rat)
Alternate Names
bA131P10.1; BM-002; C13orf20; chromosome 13 open reading frame 20; ubiquitin-fold modifier 1; UFM1

Citations for Recombinant Human UFM1 Agarose Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Reactive-site-centric chemoproteomics identifies a distinct class of deubiquitinase enzymes
    Authors: DS Hewings, J Heideker, TP Ma, AP AhYoung, F El Oualid, A Amore, GT Costakes, D Kirchhofer, B Brasher, T Pillow, N Popovych, T Maurer, C Schwerdtfe, WF Forrest, K Yu, J Flygare, M Bogyo, IE Wertz
    Nat Commun, 2018;9(1):1162.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  2. ZUFSP Deubiquitylates K63-Linked Polyubiquitin Chains to Promote Genome Stability
    Authors: P Haahr, N Borgermann, X Guo, D Typas, D Achuthanku, S Hoffmann, R Shearer, TK Sixma, N Mailand
    Mol. Cell, 2018;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay

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