Recombinant Human Universal Type I IFN Protein, CF

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Recombinant Human Universal Type I IFN Protein Bioactivity.
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Product Details

Recombinant Human Universal Type I IFN Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED50 for this effect is 6.00-60.0 pg/mL.
Human embryonic kidney cell, HEK293-derived human IFN-alpha protein
Human IFNA-2A
Accession # P01563.1
Human IFNA-1B
Accession # CAA23799.1
Accession #
N-terminal Sequence
Predicted Molecular Mass
19 kDa
16-22 kDa, under reducing conditions.

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity View Larger

Recombinant Human Universal Type I IFN (Catalog # 11020-IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 6.00‑60.0 pg/mL

SDS-PAGE View Larger

2 μg/lane of Recombinant Human Universal Type I IFN Protein (Catalog # 11020-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 16-22 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: IFN-alpha

Interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, classified based on their binding specificity to cell surface receptors (1). Human IFNA2 was originally cloned in the early ‘80s and now more than a dozen closely related IFN alpha subtypes have been identified in both the human and mouse genome, each sharing about 80% amino acid (aa) sequence homology (2-4). Structurally, type I IFNs belong to the class of five helical‑bundle cytokines, with the IFNA subtypes containing 2 conserved disulfide bonds (5). The extracellular domain (ECD) of mature human universal Type I IFN is a hybrid of the N-terminal residues of IFNA-2A and C-terminal residues of IFNA-1B. The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 (alpha -subunit) and IFNAR2 (beta -subunit) (6, 7). Individual IFNA subtypes are known to display unique efficacies to viral protection, and IFNA1 exhibits low potency, determined by both antiviral and antiproliferative activities (8). Conversely, hybrid IFNA molecules, similar to universal Type I IFN, exhibit high specific activity across multiple species (9, 10). These molecules were developed to help study the biology of the IFN system in various animal species (11). Human IFNA1 was the first IFNA to be purified and has been tested as a treatment for various diseases (12-14).

  1. Pestka, S. et al. (1987) Annu Rev Biochem. 56:727.
  2. Goeddel, D.V. et al. (1980) Nature 287:411.
  3. Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
  4. Schreiber, G. and J. Piehler (2015) Trends Immunol. 36:139.
  5. Wittling, M.C. et al. (2021) Front Immunol. 11:605673.
  6. van Pesch, V. et al. (2004) J. Virol. 78:8219.
  7. James, C.M. et al. (2007) Vaccine. 25(10):1856.
  8. Moll, H.P. et al. (2011) Cytokine. 53:52.
  9. Horisberger, M.A. and de Staritzky, K. (1987) J Gen Virol. 68:945.
  10. Hu, R. et al. (1999) J Immunol. 163:854.
  11. Horisberger, M.A. and Di Marco, S. (1995) Pharmacol Ther. 66:507.
  12. Rubinstein, M. et al. (1978) Science. 202:1289.
  13. Harper, M.S. et al. (2015) PLOS Pathogens 11:e1005254.
  14. George, J. and Mattapallil, J.J. (2018) Front Immunol. 9:299.
Long Name
Interferon alpha
Entrez Gene IDs
3440 (Human)
Alternate Names
IFNA; IFNA2; IFNA2b; IFNalpha; IFN-alpha; IFN-alphaA; INFA2; interferon alpha 2; LeIF D


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