Recombinant Human uPAR His-tag Avi-tag Protein, CF

Catalog # Availability Size / Price Qty
AVI807-100
Recombinant Human uPAR His-tag Avi-tag Protein, CF Binding Activity
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Recombinant Human uPAR His-tag Avi-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human u-Plasminogen Activator (uPA)/Urokinase (Catalog # 1310-SE) is immobilized at 5 μg/mL (100 μL/well), the concentration of Recombinant Human uPAR His-tag Avi-tag (Catalog # AVI807) that produces 50% of the optimal binding response is 40‑240 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human uPAR protein
Leu23-Arg303
Accession #
N-terminal Sequence
Analysis
Leu23
Structure / Form
Biotinylated via Avi-tag
Predicted Molecular Mass
29 kDa
SDS-PAGE
47-54 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI807

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant Human u-Plasminogen Activator (uPA)/Urokinase (Catalog # 1310-SE) is immobilized at 5 μg/mL (100 μL/well), Recombinant HumanuPAR His-tag Avi-tag (Catalog # AVI807) binds with an ED50 of 40‑240 ng/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human uPAR His-tag Avi-tag (Catalog # AVI807) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 47-54 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: uPAR

The urokinase-type Plasminogen Activator (uPA) is one of two activators that converts the extracellular zymogen plasminogen to plasmin, a serine protease that is involved in a variety of normal and pathological processes that require cell migration and/or tissue destruction. uPA is synthesized and released from cells as a single-chain (sc) pro-enzyme with limited enzymatic activity and is converted to an active two-chain (tc) disulfide-linked active enzyme by plasmin and other specific proteinases. Both the scuPA and tcuPA bind with high-affinity to the cell surface via the glycosyl phosphatidylinositol-linked receptor uPAR which serves to localize the uPA proteolytic activity. The enzymatic activity of scuPA has also been shown to be enhanced by binding to uPAR. Independent of their proteolytic activity, the uPA/uPAR interaction also initiates signal transduction responses resulting in activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis. uPAR can interact with integrins to suppress normal integrin adhesive function and promote adhesion to vitronectin through a high affinity vitronectin binding site on uPAR. uPAR cDNA encodes a 335 amino acid (aa) residue precursor protein with a 22 aa residue signal peptide, five potential N-linked glycosylation sites and a C-terminal GPI-anchor site. An alternate spliced variant of uPAR encoding a secreted soluble form of uPAR also exists. Human and mouse uPAR share approximately 60% aa sequence identity and the receptor-ligand interaction is strictly species-specific.

References
  1. Dear, A.E. and R.L. Medcalf, Eur. J. Biochemistry (1998) 252:185.
Long Name
Urokinase-type Plasminogen Activator Receptor
Entrez Gene IDs
5329 (Human); 18793 (Mouse)
Alternate Names
CD87 antigen; CD87; Monocyte activation antigen Mo3; plasminogen activator, urokinase receptor; PLAUR; uPAR; U-PAR; UPARurokinase plasminogen activator surface receptor; u-plasminogen activator receptor form 2; URKRMO3

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