Recombinant Mouse BAMBI/NMA Fc Chimera Protein, CF
Recombinant Mouse BAMBI/NMA Fc Chimera Protein, CF Summary
Accession # Q9D0L6
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
BMP and Activin Membrane-Bound Inhibitor (BAMBI), also called Non-Metastatic Gene A (NMA), is a type I transmembrane protein that shares sequence homology with the TGF-beta type I receptor family and functions as a decoy receptor (1). Mouse BAMBI is synthesized as a 260 amino acid (aa) precursor and has a predicted molecular weight of approximately 29 kDa (1). Its extracellular domain contains only one potential N-glycosylation site, and shares 91% and 98% aa sequence identity with the human and rat orthologs, respectively. BAMBI differs from other members of the TGF-beta RI family in that it has a short cytoplasmic region that lacks the intracellular serine/threonine kinase domain (1, 2). It competes with type I receptors to form heterodimers with type II receptors, thus interfering with BMP, Activin, and TGF-beta signaling (2). In fact, BAMBI is believed to be one component of a negative feedback loop for BMP signaling that serves to increase the dynamic signaling range for BMPs (3, 4). During development, BAMBI is prominently expressed in gastrulation, neurulation, and during the development of teeth and bones, and is often co-expressed with other BMP family members (1-5). BAMBI is also ubiquitously expressed in the adult, with the highest levels being observed in heart, lung, spleen, kidney, bladder, and testes (6). BAMBI expression is induced by TGF-beta and Wnt signaling (7, 8). It cooperates with inhibitory Smad6 and Smad7 to inhibit TGF-beta signaling (9). In contrast, BAMBI promotes Wnt signaling via its interactions with Frizzled receptors (10). BAMBI has been shown to modulate various processes including adipogenesis, thrombus formation and stability, cell proliferation, and opioid signaling in neuropathic pain (10-13). Additionally, aberrant BAMBI expression is believed to be a factor in the development of cancer, inflammation, and fibrotic processes (7, 14-18).
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