Recombinant Mouse BTLA Fc Chimera Protein, CF

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Recombinant Mouse BTLA Fc Chimera Protein Binding Activity
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Recombinant Mouse BTLA Fc Chimera Protein, CF Summary

Product Specifications

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured in a competitive binding assay. When Recombinant Mouse HVEM/TNFRSF14 Fc Chimera (Catalog # 2516-HV) is immobilized at 0.1 µg/mL (100 µL/well), Recombinant Mouse BTLA Fc Chimera inhibits 50% binding of biotinylated Recombinant Mouse BTLA Fc Chimera (0.2 µg/mL) at the concentration range of 0.3-1 µg/mL.
Mouse myeloma cell line, NS0-derived mouse BTLA protein
Mouse BTLA
Glu30-Gly176 (Lys173Glu)
Accession # Q32MV9
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
44 kDa (monomer)
60-70 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Mouse BTLA Fc Chimera Protein Binding Activity View Larger

When Recombinant Mouse HVEM/TNFRSF14 Fc Chimera (Catalog # 2516-HV) is coated at 0.1 µg/mL, Recombinant Mouse BTLA Fc Chimera inhibits the binding of biotinylated Recombinant Mouse BTLA Fc Chimera with an IC50of 0.3-1 µg/mL

Reconstitution Calculator

Reconstitution Calculator

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Background: BTLA

B- and T-lymphocyte attenuator (BTLA; CD272) is a 35 kDa type I transmembrane glycoprotein in the CD28 family of T cell co-stimulatory molecules (1-3). Mature mouse BTLA contains a 154 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane sequence, and a 102 aa cytoplasmic domain. The two ITIM motifs and three Tyr phosphorylation sites in the cytoplasmic tail transmit inhibitory signaling (4-5). The ECD of mouse BTLA shares 51% and 77% aa identity with that of human and rat BTLA, respectively. A splice variant lacking the transmembrane domain has been reported (6). Unlike other CD28 family members, the BTLA Ig domain in the ECD is of the I-type rather than V-type, and BTLA does not form homodimers (7). BTLA is also unusual in its interaction with the TNF superfamily member HVEM rather than with B7 family ligands (8). BTLA is expressed on T cells, B cells, macrophages, dendritic cells, and NK cells (9). Its expression is low in naïve T cells and increases during antigen-specific induction of anergy. In B cells, BTLA expression is highest in mature naïve cells (9). BTLA apparently limits T cell numbers, since its deletion results in over-production of T cells, especially CD8+ memory T cells that are hyper-responsive to TCR cross-linking (10). Under the control of ROR gamma t and IL-7, BTLA regulates the homeostasis and inflammatory responses of gamma δT cells (11). The binding of BTLA and HVEM does not preclude the concurrent binding of other HVEM ligands such as LIGHT or Lymphotoxin-alpha (12).

  1. Murphy, K.M. et al. (2006) Nat. Rev. Immunol. 6:671.
  2. Croft, M. (2005) Trends. Immunol. 26:292.
  3. Watanabe, N. et al. (2003) Nat. Immunol. 4:670.
  4. Gavrieli, M. et al. (2003) Biochem. Biophys. Res. Commun. 312:1236.
  5. Chemnitz, J.M. et al. (2006) J. Immunol. 176:6603.
  6. Han, P. et al. (2004) J. Immunol. 172 :5931.
  7. Compaan, D.M. et al. (2005) J. Biol. Chem. 280:39553.
  8. Sedy, J. R. et al. (2005) Nat. Immunol. 6:90.
  9. Hurchla, M.A. et al. (2005) J. Immunol. 174:3377.
  10. Krieg, C. et al. (2007) Nat. Immunol. 8:162.
  11. Bekiaris, V. et al. (2013) Immunity 39:1082.
  12. Cai G and Freeman GJ, (2009) Immunol Rev. 229:244
Long Name
B- And T-Lymphocyte Attenuator
Entrez Gene IDs
151888 (Human); 208154 (Mouse); 102144877 (Cynomolgus Monkey)
Alternate Names
B and T lymphocyte associated; B and T lymphocyte attenuator; B- and T-lymphocyte attenuator; B- and T-lymphocyte-associated protein; BTLA; BTLA1; CD272 antigen; CD272; FLJ16065; MGC129743

Citation for Recombinant Mouse BTLA Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. ICOS agonism by JTX-2011 (vopratelimab) requires initial T cell priming and Fc cross-linking for optimal T cell activation and anti-tumor immunity in preclinical models
    Authors: A Hanson, K Elpek, E Duong, L Shallberg, M Fan, C Johnson, M Wallace, GR Mabry, S Sazinsky, L Pepper, CJ Shu, S Sathyanara, S Zuerndorfe, T Simpson, M Gostissa, M Briskin, D Law, J Michaelson, CJ Harvey
    PLoS ONE, 2020;15(9):e0239595.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay


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