Recombinant Mouse BTNL2/Butyrophilin-like 2 Protein, CF

    
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  • Purity
    >95%, by SDS-PAGE with silver staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is 1-6 μg/mL.
  • Source
    Mouse myeloma cell line, NS0-derived mouse BTNL2/Butyrophilin-like 2 protein
    Asp27-Ser452, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Asp27
  • Predicted Molecular Mass
    49 kDa
  • SDS-PAGE
    54-67 kDa, reducing conditions
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8605-BT
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
 
Reconstitution Reconstitute at 200 μg/mL in PBS.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Data Image
Recombinant Mouse BTNL2/Butyrophilin-like 2 inhibits IL-2secretion by mouse T cells in the presence of anti-CD3 antibody. The ED50for this effect is 1-6 ug/mL.
Background: BTNL2/Butyrophilin-like 2

Butyrophilin-like 2 (BTNL2) is a member of the BTN/MOG Ig-superfamily and functions as a negative regulator of immune cell activation (1).Mouse BTNL2 is a 514 amino acid (aa) type I transmembrane glycoprotein that contains a signal peptide followed by an extracellular domain (ECD), a transmembrane region and a short cytoplasmic domain (2). The ECD features two V-type Ig-like domains, two C-type Ig-like domains, and four glycosylation sites. The ECD of mouse BTNL2 (aa 27-452) shares 64% and 88% sequence identity with the ECD of human and rat BTNL2, respectively. A splice variant of BTNL2 lacks the second Ig-like domain in the ECD (2). BTNL2 is expressed in epithelial cells of the small intestine, colonic dendritic cells, and in cells of the lymph node (1, 2). BTNL2 expression is upregulated in T cells following activation, a characteristic BTNL2 shares with the homologous B7 family of co-stimulatory molecules (3, 4). BTNL2 negatively regulates T cells by inhibiting proliferation and inflammatory cytokine secretion (1, 3). It also increases the expression of FoxP3 in T cells to promote regulatory T cell development (5). Single nucleotide polymorphisms in BTNL2 are associated with a risk for sporadic prostate cancer, rheumatoid arthritis, sarcoidosis, ulcerative colitis, and other inflammatory diseases (2, 6-12).

  • References:
    1. Arnett, H.A. et al. (2007) J. Immunol. 178:1523.
    2. Arnett, H.A. et al. (2009) Cytokine 46:370.
    3. Smith, I.A. et al. (2010) J. Immunol. 184:3514.
    4. Nguyen, T. et al. (2006) J. Immunol. 176:7354.
    5. Swanson, R.M. et al. (2013) J. Immunol. 190:2027.
    6. Morais, A. et al. (2012) Respir. Med. 106:1771.
    7. Milman, N. et al. (2011) Clin. Respir. J. 5:105.
    8. Wijnen, P.A. et al. (2011) Hum. Immunol. 72:342.
    9. Valentonyte, R. et al. (2005) Nat. Genet. 37:357.
    10. Pathan, S. et al. (2009) Tissue. Antigens 74:322.
    11. Mitsunaga, S. et al. (2013) J. Hum. Genet. 58:210.
    12. Fitzgerald, L.M. et al. (2013) Cancer Epidemiol. Biomarkers Prev. 22:1520.
  • Entrez Gene IDs:
    56244 (Human); 547431 (Mouse)
  • Alternate Names:
    BTL-II; BTNL2; Butyrophilin like 2; butyrophilin-like 2 (MHC class II associated); Butyrophilin-like 2
Related Research Areas

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Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB , Simple Western , Flow , AP , CyTOF-ready MAB050 31  
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His Tag HRP-conjugated Antibody

WB , Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag PE-conjugated Antibody

ICFlow IC050P 3  
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His Tag Antibody

WB , Flow , AP MAB050R 2  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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