Recombinant Mouse CD163 Protein, CF Summary
Val39-Thr1045, with a C-terminal 10-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
CD163, also known as M130 and p155, is a 130-160 kDa type I transmembrane protein belonging to group B of the cysteine-rich scavenger receptor family (1). Mature mouse CD163 consists of a 1007 amino acid (aa) extracellular domain (ECD) with 9 scavenger receptor cysteine-rich (SRCR) domains, a 21 aa transmembrane segment, and a 55 aa cytoplasmic domain (2). Alternative splicing of mouse CD163 generates an isoform with a substitution in the cytoplasmic region. Within the ECD, mouse CD163 shares 75% and 90% aa sequence identity with human and rat CD163, respectively. CD163 is expressed on monocytes and macrophages and is inducible by immunosuppressant glucocorticoids and IL-10 (3-5). A soluble form is shed from the cell surface by TACE or neutrophil elastase mediated cleavage (6, 7) in response to oxidative stress, Prostaglandin F2a stimulation, or the activation of Fc gamma receptors, TLR1, 2, 5, or 6 (8-10). CD163 mediates monocyte binding to bacteria, leading to the release of inflammatory cytokines (11). It is essential for the circulatory clearance of hemoglobin-haptoglobin (Hb-Hp) complexes as well as free hemoglobin (12, 13). It can also mediate monocyte-erythroblast adhesion and promote erythroblast expansion (14). CD163 binds and internalizes the cytokine TWEAK, and the ratio of soluble CD163 to TWEAK in the plasma is elevated during atherosclerosis (15, 16).
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- Moreno, J.A. et al. (2012) Eur. Heart J. 33:252.
- Weaver, L.K. et al. (2006) J. Leukoc. Biol. 80:26.
- Timmermann, M. and P. Hogger (2005) Free Radic. Biol. Med. 39:98.
- Sulahian, T.H. et al. (2004) J. Leukoc. Biol. 76:271.
- Fabriek, B.O. et al. (2009) Blood 113:887.
- Kristiansen, M. et al. (2001) Nature 409:198.
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- Bover, L.C. et al. (2007) J. Immunol. 178:8183.
- Moreno, J.A. et al. (2009) Atherosclerosis 207:103.
Citation for Recombinant Mouse CD163 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Precision Targeting of Tumor Macrophages with a CD206 Binding Peptide
Authors: P Scodeller, L Simón-Grac, S Kopanchuk, A Tobi, K Kilk, P Säälik, K Kurm, ML Squadrito, VR Kotamraju, A Rinken, M De Palma, E Ruoslahti, T Teesalu
Sci Rep, 2017;7(1):14655.
Sample Types: Recombinant Protein
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