Recombinant Mouse CD229/SLAMF3 Protein, CF Summary
Lys48-Phe454, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
CD229, also known as Ly9 and SLAMF3, is a 120 kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 family (1). Mature mouse CD229 consists of a 406 aa extracellular domain (ECD) with two Ig‑like V‑set and two Ig‑like truncated C2‑set domains, a 21 aa transmembrane segment, and a 180 aa cytoplasmic domain with two immunoreceptor tyrosine‑based switch motifs ITSMs (2, 3). The ECD of mouse CD229 shares 59% and 73% aa sequence identity with human and rat CD229, respectively. Within the first two Ig‑like domains that are common to all SLAM proteins, mouse CD229 shares 22% ‑ 36% aa sequence identity with mouse 2B4, BLAME, CD2F‑10, CD84, CRACC, NTB‑A, and SLAM. CD229 is expressed on T, B, and NK cells, thymocytes, and monocytes (2 ‑ 7). Homophilic binding between CD229 molecules is mediated by the N‑terminal Ig‑like domain (8). Human and mouse CD229 exhibit cross‑species binding (8). Antigen stimulation of lymphocytes induces CD229 clustering to sites of T cell ‑ B cell contact (8). Y470 and Y606 in the cytoplasmic domain are required for association of CD229 with the adaptor proteins AP‑2 (μ2 chain) and GRB‑2, respectively, and both tyrosines are required for CD229 internalization (9, 10). The two ITSMs, which include Y558 and Y581, mediate phosphorylation‑dependent CD229 association with SAP and SHP‑2 (11). Antibody ligation of CD229 can inhibit T cell activation, but CD229 knockout mice show impaired T cell immune responses, suggesting a potential role for CD229 in T cell activation or co‑stimulation (7, 12).
- Bhat, R. et al. (2006) J. Leukoc. Biol. 79:417.
- de la Fuente, M.A. et al. (2001) Blood 97:3513.
- Sandrin, M.S. et al. (1992) J. Immunol. 149:1636.
- Romero, X. et al. (2004) Tissue Antigens 64:132.
- Hogarth, P.M. et al. (1980) Immunogenetics 11:65.
- Mathieson, B.J. et al. (1980) J. Immunol. 125:2127.
- Sintes, J. et al. (2007) Tissue Antigens 70:355.
- Romero, X. et al. (2005) J. Immunol. 174:7033.
- Del Valle, J.M. et al. (2003) J. Biol. Chem. 278:17430.
- Martin, M. et al. (2005) J. Immunol. 174:5977.
- Sayos, J. et al. (2001) Blood 97:3867.
- Graham, D.B. et al. (2006) J. Immunol. 176:291.
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