Recombinant Mouse EphB4 Fc Chimera Protein: R&D Systems

Recombinant Mouse EphB4 Fc Chimera Protein, CF

(13 citations)   
  • Purity
    >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse EphB4 Fc Chimera at 2 µg/mL (100 µL/well) can bind Recombinant Mouse Ephrin‑B2 Fc Chimera (Catalog # 496-EB) with a linear range of 0.078-5 ng/mL.
    Optimal dilutions should be determined by each laboratory for each application.
  • Source
    Mouse myeloma cell line, NS0-derived
    Mouse EphB4
    (Leu16-Ala539)
    Accession # P54761
    DIEGRMD Human IgG1
    (Pro100-Lys330)
    6-His tag
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Leu16
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    85.2 kDa (monomer)
  • SDS-PAGE
    110 kDa, reducing conditions
446-B4
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EphB4
EphB4, also known as Htk, Myk1, Tyro11, and Mdk2 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphB4 has been shown to bind ephrin-B2 andephrin-B1 (2, 3). The extracellular domains of human and mouse EphB4 share 88% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).
  • References:
    1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
    2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
    3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.
  • Long Name:
    Eph Receptor B4
  • Entrez Gene IDs:
    2050 (Human); 13846 (Mouse)
  • Alternate Names:
    EC 2.7.10; EC 2.7.10.1; EPH receptor B4; EphB4; ephrin type-B receptor 4; hepatoma transmembrane kinase; Htk; HTKephrin receptor EphB4; Mdk2; Myk1; soluble EPHB4 variant 1; soluble EPHB4 variant 2; soluble EPHB4 variant 3; Tyro11; Tyrosine-protein kinase receptor HTK; Tyrosine-protein kinase TYRO11
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. EphrinB2 controls vessel pruning through STAT1-JNK3 signalling.
    Authors: Salvucci O, Ohnuki H, Maric D, Hou X, Li X, Yoon S, Segarra M, Eberhart C, Acker-Palmer A, Tosato G
    Nat Commun, 2015;6(0):6576.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. CHMP5 controls bone turnover rates by dampening NF-kappaB activity in osteoclasts.
    Authors: Greenblatt M, Park K, Oh H, Kim J, Shin D, Lee J, Lee J, Singh A, Lee K, Hu D, Xiao C, Charles J, Penninger J, Lotinun S, Baron R, Ghosh S, Shim J
    J Exp Med, 2015;212(8):1283-301.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  3. Limited gene expression variation in human embryonic stem cell and induced pluripotent stem cell-derived endothelial cells.
    Authors: White M, Rufaihah A, Liu L, Ghebremariam Y, Ivey K, Cooke J, Srivastava D
    Stem Cells, 2013;31(1):92-103.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Ephrin-B2 regulates VEGFR2 function in developmental and tumour angiogenesis.
    Authors: Sawamiphak S, Seidel S, Essmann CL, Wilkinson GA, Pitulescu ME, Acker T, Acker-Palmer A
    Nature, 2010;465(7297):487-91.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: IP
  5. The ephrinB2/EphB4 axis is dysregulated in osteoprogenitors from myeloma patients and its activation affects myeloma bone disease and tumor growth.
    Authors: Pennisi A, Ling W, Li X, Khan S, Shaughnessy JD, Barlogie B, Yaccoby S
    Blood, 2009;114(9):1803-12.
    Species: Human
    Sample Type: In Vivo
    Application: In Vivo
  6. Directed and systematic differentiation of cardiovascular cells from mouse induced pluripotent stem cells.
    Authors: Narazaki G, Uosaki H, Teranishi M, Okita K, Kim B, Matsuoka S, Yamanaka S, Yamashita JK
    Circulation, 2008;118(5):498-506.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  7. Fibronectin type I repeat is a nonactivating ligand for EphA1 and inhibits ATF3-dependent angiogenesis.
    Authors: Masuda J, Usui R, Maru Y
    J. Biol. Chem., 2008;283(19):13148-55.
    Species: Human
    Sample Type: Protein
    Application: Bioassay
  8. Tyrosine phosphorylation sites in ephrinB2 are required for hippocampal long-term potentiation but not long-term depression.
    Authors: Bouzioukh F, Wilkinson GA, Adelmann G, Frotscher M, Stein V, Klein R
    J. Neurosci., 2007;27(42):11279-88.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  9. Reverse endocytosis of transmembrane ephrin-B ligands via a clathrin-mediated pathway.
    Authors: Parker M, Roberts R, Enriquez M, Zhao X, Takahashi T, Pat Cerretti D, Daniel T, Chen J
    Biochem. Biophys. Res. Commun., 2004;323(1):17-23.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  10. Interplay between EphB4 on tumor cells and vascular ephrin-B2 regulates tumor growth.
    Authors: Noren NK, Lu M, Freeman AL, Koolpe M, Pasquale EB
    Proc. Natl. Acad. Sci. U.S.A., 2004;101(15):5583-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  11. Flow regulates arterial-venous differentiation in the chick embryo yolk sac.
    Authors: le Noble F, Moyon D, Pardanaud L, Yuan L, Djonov V, Matthijsen R, Breant C, Fleury V, Eichmann A
    Development, 2004;131(2):361-75.
    Species: Avian - Quail
    Sample Type: In Vivo
    Application: In Vivo
  12. Inhibition of tumor growth and angiogenesis by soluble EphB4.
    Authors: Martiny-Baron G, Korff T, Schaffner F, Esser N, Eggstein S, Marme D, Augustin HG
    Neoplasia, 2004;6(3):248-57.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  13. Forward EphB4 signaling in endothelial cells controls cellular repulsion and segregation from ephrinB2 positive cells.
    Authors: Fuller T, Korff T, Kilian A, Dandekar G, Augustin HG
    J. Cell. Sci., 2003;116(0):2461-70.
    Species: Human
    Sample Type: Cell Lysates
    Application: Affinity Chromatography
Expand to show all 13 Citations
Primary Antibodies
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His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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Recombinant Proteins
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Recombinant Human IgG1 Fc, CF

BA 110-HG 65
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Recombinant Mouse Ephrin-B2 Fc Chimera Protein, CF

Bind 496-EB 32
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