Recombinant Mouse Follistatin-like 1 Protein, CF

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R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse Follistatin-like 1 Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Follistatin‑like 1/FSTL1 is coated at 5 μg/mL (100 μL/well), the concentration of Recombinant Mouse BMP‑4 (Catalog # 5020-BP) that produces 50% of the optimal binding response is approximately 30-150 ng/mL.
Mouse myeloma cell line, NS0-derived mouse Follistatin-like 1/FSTL1 protein
Glu19-Ile306, with a C-terminal Asp-Ile and 6-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
33.5 kDa
43-50 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Follistatin-like 1/FSTL1

Follistatin-like 1 (FSTL1 or FSL1), also known as FRP (follistatin-related protein), Flik (follistatin-like), and TSC-36 (TGF-beta 1-stimulated clone 36), is a secreted 45‑55 kDa extracellular glycoprotein belonging to the BM-40/SPARC/Osteonectin family (1-3). The mouse FSTL1 cDNA encodes 306 amino acids (aa), including an 18 aa signal sequence, a cysteine-rich Follistatin (EGF- and kazal-like) domain, two apparently nonfunctional EF-hand calcium-binding motifs, and a von Willebrand Factor C homology domain (1, 3). Mature mouse FSTL1 shares 94%, 96%, 94%, 93% and 93% aa identity with human, rat, equine, bovine and canine FSTL1, respectively. FSTL1 was first identified as a TGF-beta -induced protein from a mouse osteoblast cell line (4). It is ubiquitously expressed in early mouse development, but is mainly mesenchymal later in development (5). In vitro, FSTL1 interacts directly with BMP-4, while in vivo, it regulates BMP-4 signaling during normal lung development, as demonstrated by lethal respiratory failure in mice deleted for FSTL1 (6). In humans, FSTL1 is a common rheumatoid arthritis autoantigen (2). It is reported to be either proinflammatory due to promoting inflammatory cytokine secretion, or to prevent autoimmune arthritis by inhibiting matrix metalloproteinase (MMP) and prostaglandin expression (7-10). In muscle and heart, it appears to be protective and promotes endothelial cell functions such as revascularization after ischemia, probably due to promoting expression and activation of the protein kinase AKT1 (11, 12). Cardiac and circulating FSTL1 is generally increased in conditions such as heart failure and acute coronary syndrome (12-14). FSTL1 also appears to be a tumor suppressor, showing down‑regulated expression in many human cancers (4, 15, 16). In vitro, it slows proliferation and MMP-dependent migration, and increases FAS-dependent apoptosis of tumor cell lines (15).

  1. Zwijsen, A. et al. (1994) Eur. J. Biochem. 225:937.
  2. Tanaka, M. et al. (1998) Int. Immunol. 10:1305.
  3. Hambrook, H.O. et al. (2004) J. Biol. Chem. 279:11727.
  4. Shibanuma, M. et al. (1993) Eur. J. Biochem. 217:13.
  5. Adams, D. et al. (2007) Gene Expr. Patterns 7:491.
  6. Geng, Y et al. (2011) Proc. Natl. Acad. Sci. USA 108:7058.
  7. Clutter, S.D. et al. (2009) J. Immunol. 182:234.
  8. Miyamae, T. et al. (2006) J. Immunol. 177:4758.
  9. Kawabata, D. et al. (2004) Arthritis Rheum. 50:660.
  10. Tanaka, M. et al. (2003) Int. Immunol. 15:71.
  11. Ouchi, N. et al. (2008) J. Biol. Chem. 283:32802.
  12. Oshima, Y. et al. (2008) Circulation 117:3099.
  13. Lara-Pezzi, E. et al. (2008) Endocrinology 149:5822.
  14. Widera, C. et al. (2009) Clin. Chem. 55:1794.
  15. Chan, Q.K. et al. (2009) Carcinogenesis 30:114.
  16. Mashimo, J. et al. (1997) Cancer Lett. 113:213.
Entrez Gene IDs
11167 (Human); 14314 (Mouse); 79210 (Rat)
Alternate Names
Flik; FLJ50214; Follistatin-like 1; Follistatin-like protein 1; follistatin-related protein 1; Follistatin-related Protein; FRP; FRPFLJ52277; FSL1; FSTL1; TSC-36

Citations for Recombinant Mouse Follistatin-like 1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Follistatin-like 1 (FSTL1) interacts with Wnt ligands and Frizzled receptors to enhance Wnt/beta-catenin signaling in obstructed kidneys in vivo
    Authors: Y Zhang, Y Wang, G Zheng, Y Liu, J Li, H Huang, C Xu, Y Zeng, X Zhang, J Qin, C Dai, HO Hambrock, U Hartmann, B Feng, KK Mak, Y Liu, HY Lan, Y Huang, ZH Zheng, Y Xia
    The Journal of Biological Chemistry, 2022;0(0):102010.
    Species: Mouse
    Sample Types: Whole Cell
    Applications: Cell Culture
  2. Ups and downs: the PPARgamma/p-PPARgamma seesaw of follistatin-like 1 and integrin receptor signaling in adipogenesis
    Authors: D Fang, X Shi, X Jia, C Yang, L Wang, B Du, T Lu, L Shan, Y Gao
    Molecular Metabolism, 2021;0(0):101400.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. BBS4 regulates the expression and secretion of FSTL1, a protein that participates in ciliogenesis and the differentiation of 3T3-L1
    Authors: V Prieto-Ech, S Lodh, L Colman, N Bobba, L Santos, N Katsanis, C Escande, NA Zaghloul, JL Badano
    Sci Rep, 2017;7(1):9765.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Intratumoural heterogeneity generated by Notch signalling promotes small-cell lung cancer
    Authors: JS Lim, A Ibaseta, MM Fischer, B Cancilla, G O'Young, S Cristea, VC Luca, D Yang, NS Jahchan, C Hamard, M Antoine, M Wislez, C Kong, J Cain, YW Liu, AM Kapoun, KC Garcia, T Hoey, CL Murriel, J Sage
    Nature, 2017;545(7654):360-364.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Follistatin-like 1: a potential mediator of inflammation in obesity.
    Authors: Fan N, Sun H, Wang Y, Wang Y, Zhang L, Xia Z, Peng L, Hou Y, Shen W, Liu R, Yin J, Peng Y
    Mediators Inflamm, 2013;2013(0):752519.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay


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Recombinant Mouse Follistatin-like 1 Protein, CF
By Pawel Mazur on 10/21/2016
Application: In vitro bioactivity in cell culture