Recombinant Mouse Glypican 3 Protein, CF

Catalog # Availability Size / Price Qty
6938-GP-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse Glypican 3 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Glypican 3 is coated at 5 μg/mL (100 μL/well), the concentration of recombinant human FGF basic that produces 50% of the optimal binding response is found to be approximately 0.75-3.75 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Glypican 3 protein
Gln25-Met557 & Ser358-Met557, both with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
 Gln25 predicted (No result obtained, sequencing might be blocked) & Ser358
Predicted Molecular Mass
61.3 kDa
SDS-PAGE
66-98 kDa & 30-40 kDa, reducing conditions

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6938-GP

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6938-GP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Glypican 3

The Glypicans (GPCs) are a small multigene family of GPI-linked proteoglycans that play a key role in growth factor signaling (1 ‑ 4). Glypicans exhibit a 60 ‑ 70 kDa protein core that consists of a globular N‑terminus, 14 conserved cysteines that form multiple intrachain disulfide bonds, and a number of C‑terminal N‑ and O‑linked carbohydrate attachment sites. At least two subfamilies of Glypicans are known (GPC1, 2, 4, and 6 and GPC3 and 5) (1, 5). Mouse Glypican 3 is synthesized as a 579 amino acid (aa) preproprecursor that contains a 24 aa signal sequence, a 535 aa mature segment, and a 20 aa C-terminal prosegment (6 ‑ 8). There are three potential N-linked, and two potential O-linked sites for glycosylation or glycanation. O-linked glycanation utilizes heparan sulfate (HS) and this contributes 60 ‑ 120 kDa to an overall molecular weight of approximately 200 kDa for Glypican 3 (9, 10). Glypican 3 undergoes convertase‑mediated proteolytic processing between Arg357 ‑ Ser358 to generate a disulfide-linked cell-surface heterodimer (9). It may also undergo Notum cleavage of the GPI-anchor to create a soluble circulating form (11, 12). Mature mouse Glypican 3 shares 95% and 99% aa identity with mature human and rat Glypican 3, respectively. Sources for Glypican 3 include liver carcinoma cells plus mesoderm and mesodermal derivatives. Cell types in mice include mesenchyme, prechondrocytes, primitive hematopoietic progenotors, fetal hepatocytes, and pericardium (6, 13). Glypican 3 is best known in human for its association with SGB syndrome, a condition characterized by tissue overgrowth (dysmorphogenesis), potentially through its binding to CD26. Normally, Glypican 3 binds to and down-regulates cell surface CD26.  In the absence of Glypican 3, CD26 promotes cell proliferation, possibly resulting in tissue malformation(s) (14 ‑ 16). Glypican 3 makes use of its both its core protein and HS adduct to impact select growth factor activity. The core protein is known to bind to Wnt and stabilize the Wnt:Frizzled interactions (1, 17, 18). It also binds to hedgehog (HH), serving as a competitive inhibitor for Patched. This results in the internalization and degradation of circulating HH, and it also removes a soluble promoter of Smoothened signaling (1, 19). The HS component of Glypican 3, by contrast, is reported to interact with members of the FGF system, promoting FGF receptor dimerization and signaling (1, 2). Finally, Glypican 3 binds to surface membrane Glut4, facilitating its stabilization and promoting the transport of glucose into the cell (20).

References
  1. Filmis, J. et al. (2008) Genome Biol. 9:224.
  2. Fransson, L-A. et al. (2004) Cell. Mol. Life Sci. 61:1016.
  3. Fransson, L-A. (2003) Int. J. Biochem. Cell Biol. 35:125.
  4. Lamoureux, F. et al. (2007) BioEssays 29:758.
  5. Veugelers, M. et al. (1999) J. Biol. Chem. 274:26968.
  6. Pellegrini, M. et al. (1998) Dev. Dyn. 213:431.
  7. Watanabe, K. et al. (1995) J. Cell Biol. 130:1207.
  8. Ho, M. and H. Kim (2011) Eur. J. Cancer 47:333.
  9. Capurro, M.I. et al. (2005) J. Biol. Chem. 280:41201.
  10. Filmus, J. et al. (1995) Biochem. J. 311:561.
  11. Traister, A. et al. (2008) Biochem. J. 410:503.
  12. Liu, H. et al. (2010) World J. Gastroenterol. 16:4410.
  13. Viviano, B.L. et al. (2005) Dev. Biol. 282:152.
  14. Gonzales, A.D. et al. (1998) J. Cell Biol. 141:1407.
  15. Cano-Gauci, D.F. et al. (1999) J. Cell Biol. 146:255.
  16. Cavoodi, J. et al. (2007) Proteomics 7:2300.
  17. Song, H.H. et al. (2005) J. Biol. Chem. 280:2116.
  18. Capurro, M.I. et al. (2005) Cancer Res. 65:6245.
  19. Capurro, M.I. et al. (2008) Dev. Cell 14:700.
  20. Taguchi, A. et al. (2008) Biochem. Biophys. Res. Commun. 369:1204.
Entrez Gene IDs
2719 (Human); 14734 (Mouse); 25236 (Rat); 102137748 (Cynomolgus Monkey)
Alternate Names
DGSX; Glypican 3; glypican proteoglycan 3; glypican-3; GPC3; GTR2-2; heparan sulphate proteoglycan; Intestinal protein OCI-5; MXR7; OCI5; OCI-5; secreted glypican-3; SGB; SGBS; SGBS1SDYS

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