Recombinant Mouse IL-4R alpha Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
530-MR-100
Product Details
Citations (2)
FAQs
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Recombinant Mouse IL-4R alpha Fc Chimera Protein, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 0.05‑0.2 µg/mL in the presence of 3 ng/mL of recombinant mouse IL-4.
Source
Mouse myeloma cell line, NS0-derived mouse IL-4 R alpha protein
Mouse IL-4 R alpha
(Ile26 - Arg233)
Accession # Q544A9
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus


Accession #
N-terminal Sequence
Analysis
Ile26
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
51 kDa
SDS-PAGE
65-85 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

530-MR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

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Background: IL-4R alpha

Interleukin 4 Receptor alpha (IL-4 R alpha ), also known as CD124 and BSF receptor, is a widely expressed 140 kDa transmembrane glycoprotein in the class I cytokine receptor family. IL-4 R alpha plays an important role in Th2-biased immune responses, alternative macrophage activation, mucosal immunity, allergic inflammation, tumor progression, and atherogenesis (1 ‑ 5). Mature mouse IL-4 R alpha consists of a 208 amino acid (aa) extracellular domain (ECD) that contains a ligand binding region and one fibronectin type-III domain, a 24 aa transmembrane segment, and a 553 aa cytoplasmic domain that contains one Box 1 motif and one ITIM motif (6). Within the ECD, mouse IL-4 R alpha shares 51% and 76% aa sequence identity with human and rat IL-4 R alpha, respectively. Alternate splicing of mouse IL-4 R alpha generates a secreted isoform and an isoform that lacks the cytoplasmic region (6 - 8). Proteolytic cleavage can also release soluble IL-4 R alpha, and both these mechanisms produce a molecule which retains ligand binding properties and inhibits IL-4 bioactivity (6, 7, 9). IL-4 R alpha is a component of two distinct receptor complexes and shows species selectivity between human and mouse (10). It can associate with the common gamma chain ( gamma c) to form the IL-4 responsive type I receptor in which gamma c increases the affinity for IL-4 and enables signaling (11, 12). It can alternatively associate with IL-13 R alpha 1 to form the type II receptor which is responsive to both IL-4 and IL-13 (13, 14). The use of shared receptor components contributes to the overlapping biological effects of IL-4 and IL-13 as well as other cytokines that utilize gamma c (i.e. IL-2, IL-7, IL-9, IL-15, and IL-21) (15, 16).

References
  1. Wills-Karp, M. and F.D. Finkelman (2008) Sci. Signal. 1:pe55.
  2. Gordon, S. and F.O. Martinez (2010) Immunity 32:593.
  3. Kuperman, D.A. and R.P. Schleimer (2008) Curr. Mol. Med. 8:384.
  4. Li, Z. et al. (2009) Cell. Mol. Immunol. 6:415.
  5. Lee, Y.W. et al. (2010) Biomol. Ther. 18:135.
  6. Mosley, B. et al. (1989) Cell 59:335.
  7. Blum, H. et al. (1996) J. Immunol. 157:1846.
  8. Wrighton, N. et al. (1992) Growth Factors 6:103.
  9. Jung, T. et al. (1999) Int. Arch. Allergy Immunol. 119:23.
  10. Idzerda, R.L. et al. (1990) J. Exp. Med. 171:861.
  11. Kondo, M. et al. (1993) Science 262:1874.
  12. Russell, S.M. et al. (1993) Science 262:1880.
  13. Hilton, D.J. et al. (1996) Proc. Natl. Acad. Sci. 93:497.
  14. Aman, M.J. et al. (1996) J. Biol. Chem. 271:29265.
  15. Ramalingam, T.R. et al. (2008) Nat. Immunol. 9:25.
  16. Overwijk, W.W. and K.S. Schluns (2009) Clin. Immunol. 132:153.
Long Name
Interleukin 4 Receptor alpha
Entrez Gene IDs
3566 (Human); 16190 (Mouse)
Alternate Names
CD124 antigen; CD124; IL-4 R alpha; IL-4 receptor subunit alpha; IL4R alpha; IL-4R alpha; IL-4R subunit alpha; IL4R; IL-4Ra; IL4RACD124; IL-4R-alpha; interleukin 4 receptor; interleukin-4 receptor alpha chain; interleukin-4 receptor subunit alpha

Citations for Recombinant Mouse IL-4R alpha Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Murine IL-4?2 splice variant down-regulates IL-4 activities independently of IL-4R? binding and STAT-6 phosphorylation
    Authors: GR Diogo, A Sparrow, MJ Paul, A Copland, PJ Hart, S Stelter, C van Dollew, PMW Drake, DC Macallan, R Reljic
    Cytokine, 2017;99(0):154-162.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: ELISA
  2. IL4 receptor ILR4alpha regulates metastatic colonization by mammary tumors through multiple signaling pathways.
    Authors: Venmar K, Carter K, Hwang D, Dozier E, Fingleton B
    Cancer Res, 2014;74(16):4329-40.

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