Recombinant Mouse IL-4R alpha Fc Chimera Protein, CF
Recombinant Mouse IL-4R alpha Fc Chimera Protein, CF Summary
|Mouse IL-4 R alpha
(Ile26 - Arg233)
Accession # Q544A9
|IEGRMD||Human IgG1 |
(Pro100 - Lys330)
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: IL-4R alpha
Interleukin 4 Receptor alpha (IL-4 R alpha ), also known as CD124 and BSF receptor, is a widely expressed 140 kDa transmembrane glycoprotein in the class I cytokine receptor family. IL-4 R alpha plays an important role in Th2-biased immune responses, alternative macrophage activation, mucosal immunity, allergic inflammation, tumor progression, and atherogenesis (1 ‑ 5). Mature mouse IL-4 R alpha consists of a 208 amino acid (aa) extracellular domain (ECD) that contains a ligand binding region and one fibronectin type-III domain, a 24 aa transmembrane segment, and a 553 aa cytoplasmic domain that contains one Box 1 motif and one ITIM motif (6). Within the ECD, mouse IL-4 R alpha shares 51% and 76% aa sequence identity with human and rat IL-4 R alpha, respectively. Alternate splicing of mouse IL-4 R alpha generates a secreted isoform and an isoform that lacks the cytoplasmic region (6 - 8). Proteolytic cleavage can also release soluble IL-4 R alpha, and both these mechanisms produce a molecule which retains ligand binding properties and inhibits IL-4 bioactivity (6, 7, 9). IL-4 R alpha is a component of two distinct receptor complexes and shows species selectivity between human and mouse (10). It can associate with the common gamma chain ( gamma c) to form the IL-4 responsive type I receptor in which gamma c increases the affinity for IL-4 and enables signaling (11, 12). It can alternatively associate with IL-13 R alpha 1 to form the type II receptor which is responsive to both IL-4 and IL-13 (13, 14). The use of shared receptor components contributes to the overlapping biological effects of IL-4 and IL-13 as well as other cytokines that utilize gamma c (i.e. IL-2, IL-7, IL-9, IL-15, and IL-21) (15, 16).
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- Gordon, S. and F.O. Martinez (2010) Immunity 32:593.
- Kuperman, D.A. and R.P. Schleimer (2008) Curr. Mol. Med. 8:384.
- Li, Z. et al. (2009) Cell. Mol. Immunol. 6:415.
- Lee, Y.W. et al. (2010) Biomol. Ther. 18:135.
- Mosley, B. et al. (1989) Cell 59:335.
- Blum, H. et al. (1996) J. Immunol. 157:1846.
- Wrighton, N. et al. (1992) Growth Factors 6:103.
- Jung, T. et al. (1999) Int. Arch. Allergy Immunol. 119:23.
- Idzerda, R.L. et al. (1990) J. Exp. Med. 171:861.
- Kondo, M. et al. (1993) Science 262:1874.
- Russell, S.M. et al. (1993) Science 262:1880.
- Hilton, D.J. et al. (1996) Proc. Natl. Acad. Sci. 93:497.
- Aman, M.J. et al. (1996) J. Biol. Chem. 271:29265.
- Ramalingam, T.R. et al. (2008) Nat. Immunol. 9:25.
- Overwijk, W.W. and K.S. Schluns (2009) Clin. Immunol. 132:153.
Citations for Recombinant Mouse IL-4R alpha Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Murine IL-4?2 splice variant down-regulates IL-4 activities independently of IL-4R? binding and STAT-6 phosphorylation
Authors: GR Diogo, A Sparrow, MJ Paul, A Copland, PJ Hart, S Stelter, C van Dollew, PMW Drake, DC Macallan, R Reljic
Sample Types: Recombinant Protein
IL4 receptor ILR4alpha regulates metastatic colonization by mammary tumors through multiple signaling pathways.
Authors: Venmar K, Carter K, Hwang D, Dozier E, Fingleton B
Cancer Res, 2014;74(16):4329-40.
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