Recombinant Mouse IL-5 Protein, CF

Formulations:
Catalog # Availability Size / Price Qty
405-ML-005/CF
405-ML-025/CF
Product Details
Citations (36)
FAQs
Reviews

Recombinant Mouse IL-5 Protein, CF Summary

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.04-0.15 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse IL-5 protein
Met21-Gly133
Accession #
N-terminal Sequence
Analysis
Met21
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
13.1 kDa (monomer)
SDS-PAGE
12-20 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

405-ML/CF

Formulation Supplied as a 0.2 μm filtered solution in PBS.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.

405-ML

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-5

Interleukin-5 (IL-5) is a secreted glycoprotein that belongs to the alpha -helical group of cytokines (1 ‑ 3). Unlike other family members, it is present as a covalently linked antiparallel dimer (4, 5). The cDNA for mouse IL‑5 encodes a signal peptide and a 113 amino acid (aa) mature protein. Mature mouse IL‑5 shares 70%, 94%, 58%, 66%, 59% and 63%, aa sequence identity with human, rat, canine, equine, feline and porcine IL-5, respectively, and shows cross-reactivity with human IL-5 receptor. IL-5 is primarily produced by CD4+ Th2 cells, but also by activated eosinophils, mast cells, EBV-transformed B cells, Reed-Sternberg cells in Hodgkin’s disease, and IL-2-stimulated invariant natural killer T cells (iNKT) (1 ‑ 3, 6 ‑ 8). IL-5 increases production and mobilization of eosinophils and CD34+ progenitors from the bone marrow and causes maturation of eosinophil precursors outside the bone marrow (1, 6, 9, 10). The receptor for human IL-5, mainly expressed by eosinophils, but also found on basophils and mast cells, consists of a unique ligand-binding subunit (IL-5 R alpha ) and a shared signal‑transducing subunit, beta c (3, 6, 11). IL-5 R alpha first binds IL-5 at low affinity, then associates with preformed beta c dimers, forming a high-affinity receptor (12). IL-5 also binds proteoglycans, potentially enhancing its activity (13). Soluble forms of IL-5 R alpha   antagonize IL-5 and can be found in vivo (10, 14). In humans, IL-5 primarily affects cells of the eosinophilic lineage, and promotes their differentiation, maturation, activation, migration and survival, while in mice IL-5 also enhances Ig class switching and release from B1 cells (1 ‑ 3, 9, 10, 15, 16). IL-5 also promotes differentiation of basophils and primes them for histamine and leukotriene release (17).

References
  1. Rosenberg, H. F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.
  2. Elsas, P.X. and M. I. G. Elsas (2007) Curr. Med. Chem. 14:1925.
  3. Martinez-Moczygemba, M. and D. P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
  4. Minamitake, Y. et al. (1990) J. Biochem. 107:292.
  5. McKenzie, A. N. et al. (1991) Mol. Immunol. 28:155.
  6. Shakoory, B. et al. (2004) J. Interferon Cytokine Res. 24:271.
  7. Lalani, T. et al. (1999) Ann. Allergy Asthma Immunol. 82:317.
  8. Sakuishi, K. et al. (2007) J. Immunol. 179:3452.
  9. Clutterbuck, E. J. et al. (1989) Blood 73:1504.
  10. Cameron, L. et al. (2000) J. Immunol. 164:1538.
  11. Tavernier, J. et al. (1991) Cell 66:1175.
  12. Zaks-Zilberman, M. et al. (2008) J. Biol. Chem. 283:13398.
  13. Lipscombe, R. et al. (1998) J. Leukocyte Biol. 63:342.
  14. Tavernier, J. et al. (2000) Blood 95:1600.
  15. Kopf, M. et al. (1996) Immunity 4:15.
  16. Horikawa, K. and K. Takatsu (2006) Immunology 118:497.
  17. Denburg, J. A. et al. (1991) Blood 77:1462.
Long Name
Interleukin 5
Entrez Gene IDs
3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)
Alternate Names
BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I

Citations for Recombinant Mouse IL-5 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

36 Citations: Showing 1 - 10
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  1. Microbiota-driven interleukin-17-producing cells and eosinophils synergize to accelerate multiple myeloma progression
    Authors: A Calcinotto, A Brevi, M Chesi, R Ferrarese, L Garcia Per, M Grioni, S Kumar, VM Garbitt, ME Sharik, KJ Henderson, G Tonon, M Tomura, Y Miwa, E Esplugues, RA Flavell, S Huber, F Canducci, VS Rajkumar, PL Bergsagel, M Bellone
    Nat Commun, 2018;9(1):4832.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. mTOR complexes differentially orchestrates eosinophil development in allergy
    Authors: C Zhu, L Xia, F Li, L Zhou, Q Weng, Z Li, Y Wu, Y Mao, C Zhang, Y Wu, M Li, S Ying, Z Chen, H Shen, W Li
    Sci Rep, 2018;8(1):6883.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Mining the Plasma Cell Transcriptome for Novel Cell Surface Proteins
    Authors: S Trezise, A Karnowski, PL Fedele, S Mithraprab, Y Liao, K D'Costa, AJ Kueh, MP Hardy, CM Owczarek, MJ Herold, A Spencer, W Shi, SN Willis, SL Nutt, LM Corcoran
    Int J Mol Sci, 2018;19(8):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. The Esophageal Organoid System Reveals Functional Interplay Between Notch and Cytokines in Reactive Epithelial�Changes
    Authors: Y Kasagi, PM Chandramou, KA Whelan, K Tanaka, V Giroux, M Sharma, J Wang, AJ Benitez, M DeMarshall, JW Tobias, KE Hamilton, GW Falk, JM Spergel, AJ Klein-Szan, AK Rustgi, AB Muir, H Nakagawa
    Cell Mol Gastroenterol Hepatol, 2018;5(3):333-352.
    Species: Mouse
    Sample Types: Organoids
    Applications: Bioassay
  5. Murine eosinophil development and allergic lung eosinophilia are largely dependent on the signaling adaptor GRB2
    Authors: R Willebrand, A Dietschman, L Nitschke, S Krappmann, D Voehringer
    Eur. J. Immunol., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. B cell activation and plasma cell differentiation are inhibited by de novo DNA methylation
    Authors: BG Barwick, CD Scharer, RJ Martinez, MJ Price, AN Wein, RR Haines, APR Bally, JE Kohlmeier, JM Boss
    Nat Commun, 2018;9(1):1900.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Plasma cell differentiation is controlled by multiple cell division-coupled epigenetic programs
    Authors: CD Scharer, BG Barwick, M Guo, APR Bally, JM Boss
    Nat Commun, 2018;9(1):1698.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. The Small Rho GTPase TC10 Modulates B Cell Immune Responses
    Authors: M Burbage, SJ Keppler, B Montaner, PK Mattila, FD Batista
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Elevating adipose eosinophils in obese mice to physiologically normal levels does not rescue metabolic impairments
    Authors: WR Bolus, KR Peterson, MJ Hubler, AJ Kennedy, ML Gruen, AH Hasty
    Mol Metab, 2017;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  10. Therapeutic reversal of food allergen sensitivity by mature retinoic acid-differentiated dendritic cell induction of LAG3(+)CD49b(-)Foxp3(-) regulatory T�cells
    Authors: W Dawicki, C Li, J Town, X Zhang, JR Gordon
    J. Allergy Clin. Immunol., 2017;139(5):1608-1620.e3.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA (Standard)
  11. IL33-mediated ILC2 activation and neutrophil IL5 production in the lung response after severe trauma: A reverse translation study from a human cohort to a mouse trauma model
    Authors: J Xu, J Guardado, R Hoffman, H Xu, R Namas, Y Vodovotz, L Xu, M Ramadan, J Brown, HR Turnquist, TR Billiar
    PLoS Med., 2017;14(7):e1002365.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Eosinophil differentiation in the bone marrow is promoted by protein tyrosine phosphatase SHP2
    Authors: LX Xia, W Hua, Y Jin, BP Tian, ZW Qiu, C Zhang, LQ Che, HB Zhou, YF Wu, HQ Huang, F Lan, YH Ke, JJ Lee, W Li, SM Ying, ZH Chen, HH Shen
    Cell Death Dis, 2016;7(0):e2175.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Eosinophil-specific deletion of IkappaBalpha in mice reveals a critical role of NF-kappaB-induced Bcl-xL for inhibition of apoptosis.
    Authors: Schwartz C, Willebrand R, Huber S, Rupec R, Wu D, Locksley R, Voehringer D
    Blood, 2015;125(25):3896-904.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. IL-25 inhibits atherosclerosis development in apolipoprotein E deficient mice.
    Authors: Mantani P, Duner P, Bengtsson E, Alm R, Ljungcrantz I, Soderberg I, Sundius L, To F, Nilsson J, Bjorkbacka H, Fredrikson G
    PLoS ONE, 2015;10(1):e0117255.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  15. The generation of neutrophils in the bone marrow is controlled by autophagy.
    Authors: Rozman S, Yousefi S, Oberson K, Kaufmann T, Benarafa C, Simon H
    Cell Death Differ, 2015;22(3):445-56.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Expression profiling of differentiating eosinophils in bone marrow cultures predicts functional links between microRNAs and their target mRNAs.
    Authors: Yang M, Eyers F, Xiang Y, Guo M, Young I, Rosenberg H, Foster P
    PLoS ONE, 2014;9(5):e97537.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Bcl-xL protein protects from C/EBP homologous protein (CHOP)-dependent apoptosis during plasma cell differentiation.
    Authors: Gaudette B, Iwakoshi N, Boise L
    J Biol Chem, 2014;289(34):23629-40.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Follicular dendritic cell secreted protein FDC-SP controls IgA production.
    Authors: Hou S, Landego I, Jayachandran N, Miller A, Gibson I, Ambrose C, Marshall A
    Mucosal Immunol, 2014;7(4):948-57.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Basophils regulate the recruitment of eosinophils in a murine model of irritant contact dermatitis.
    Authors: Nakashima C, Otsuka A, Kitoh A, Honda T, Egawa G, Nakajima S, Nakamizo S, Arita M, Kubo M, Miyachi Y, Kabashima K
    J Allergy Clin Immunol, 2014;134(1):100-7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Alternative splice variants of AID are not stoichiometrically present at the protein level in chronic lymphocytic leukemia.
    Authors: Rebhandl S, Huemer M, Zaborsky N, Gassner F, Catakovic K, Felder T, Greil R, Geisberger R
    Eur J Immunol, 2014;44(7):2175-87.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Bee venom phospholipase A2 induces a primary type 2 response that is dependent on the receptor ST2 and confers protective immunity.
    Authors: Palm N, Rosenstein R, Yu S, Schenten D, Florsheim E, Medzhitov R
    Immunity, 2013;39(5):976-85.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Homologous recombination into the eosinophil peroxidase locus generates a strain of mice expressing Cre recombinase exclusively in eosinophils.
    Authors: Doyle, Alfred D, Jacobsen, Elizabet, Ochkur, Sergei I, Willetts, Lian, Shim, Kelly, Neely, Joseph, Kloeber, Jake, Lesuer, Will E, Pero, Ralph S, Lacy, Paige, Moqbel, Redwan, Lee, Nancy A, Lee, James J
    J Leukoc Biol, 2013;94(1):17-24.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Regulation of innate CD8+ T-cell activation mediated by cytokines.
    Proc Natl Acad Sci U S A, 2012;109(25):9971-6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Defective eosinophil hematopoiesis ex vivo in inbred Rocky Mountain White (IRW) mice.
    Authors: Dyer KD, Garcia-Crespo KE, Percopo CM, Bowen AB, Ito T, Peterson KE, Gilfillan AM, Rosenberg HF
    J. Leukoc. Biol., 2011;90(6):1101-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Impaired basophil induction leads to an age-dependent innate defect in type 2 immunity during helminth infection in mice.
    Authors: Nel HJ, Hams E, Saunders SP
    J. Immunol., 2011;186(8):4631-9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  26. Innate lymphoid cells promote lung-tissue homeostasis after infection with influenza virus.
    Authors: Monticelli LA, Sonnenberg GF, Abt MC
    Nat. Immunol., 2011;12(11):1045-54.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  27. The sensing of environmental stimuli by follicular dendritic cells promotes immunoglobulin A generation in the gut.
    Authors: Suzuki K, Maruya M, Kawamoto S
    Immunity, 2010;33(1):71-83.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  28. Interleukin-1 family cytokines as mucosal vaccine adjuvants for induction of protective immunity against influenza virus.
    Authors: Kayamuro H, Yoshioka Y, Abe Y, Arita S, Katayama K, Nomura T, Yoshikawa T, Kubota-Koketsu R, Ikuta K, Okamoto S, Mori Y, Kunisawa J, Kiyono H, Itoh N, Nagano K, Kamada H, Tsutsumi Y, Tsunoda S
    J. Virol., 2010;84(24):12703-12.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  29. Sphingosine 1-phosphate regulates the egress of IgA plasmablasts from Peyer's patches for intestinal IgA responses.
    Authors: Gohda M, Kunisawa J, Miura F, Kagiyama Y, Kurashima Y, Higuchi M, Ishikawa I, Ogahara I, Kiyono H
    J. Immunol., 2008;180(8):5335-43.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  30. B7-DC induced by IL-13 works as a feedback regulator in the effector phase of allergic asthma.
    Authors: Matsumoto K, Fukuyama S, Eguchi-Tsuda M, Nakano T, Matsumoto T, Matsumura M, Moriwaki A, Kan-o K, Wada Y, Yagita H, Shin T, Pardoll DM, Patcharee R, Azuma M, Nakanishi Y, Inoue H
    Biochem. Biophys. Res. Commun., 2007;365(1):170-5.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  31. Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile.
    Authors: Wen H, Hogaboam CM, Gauldie J, Kunkel SL
    Am. J. Pathol., 2006;168(6):1940-50.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  32. The IgE adjuvant effect of particles: characterisation of the primary cellular response in the draining lymph node.
    Authors: Nygaard UC, Ormstad H, Aase A, Lovik M
    Toxicology, 2005;206(2):181-93.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  33. Arthropod-derived histamine-binding protein prevents murine allergic asthma.
    Authors: Couillin I, Maillet I, Jacobs M, Paesen GC, Moser R, Weston-Davies W
    J. Immunol., 2004;173(5):3281-6.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  34. Marked airway eosinophilia prevents development of airway hyper-responsiveness during an allergic response in IL-5 transgenic mice.
    Authors: Kobayashi T, Iijima K, Kita H
    J. Immunol., 2003;170(11):5756-63.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  35. AMD3100, a CxCR4 antagonist, attenuates allergic lung inflammation and airway hyperreactivity.
    Authors: Lukacs NW, Berlin A, Schols D, Skerlj RT, Bridger GJ
    Am. J. Pathol., 2002;160(4):1353-60.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  36. The effects of intranasal budesonide on allergen-induced production of interleukin-5 and eotaxin, airways, blood, and bone marrow eosinophilia, and eosinophil progenitor expansion in sensitized mice.
    Authors: O&amp;amp;apos;Byrne PM
    Am. J. Respir. Crit. Care Med., 2002;166(2):146-53.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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