Recombinant Mouse Leptin R Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
7814-LR-050
Product Details
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Recombinant Mouse Leptin R Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit Leptin-dependent proliferation of BaF3 mouse pro‑B cells transfected with human Leptin R. The ED50 for this effect is 2-10 ng/mL in the presence of 1 ng/mL recombinant mouse Leptin.
Source
Mouse myeloma cell line, NS0-derived mouse Leptin R protein
Mouse Leptin R
(Leu22-Gly839)
Accession # P48356
IIEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Leu22
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
119.6 kDa (monomer)
SDS-PAGE
125-190 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7814-LR

Formulation Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: Leptin R

The Leptin receptor (Leptin R; gene name LEPR), also called OB R (obesity receptor), is a 150 kDa protein that is a member of the Class I cytokine receptor family. It mediates the activities of Leptin, a multi-functional hormone produced primarily by adipose tissues that plays roles in food intake, energy metabolism, angiogenesis, reproduction, hematopoiesis, bone metabolism, and immune function (1‑3). The mouse Leptin R gene encodes 1162 amino acids (aa) including a signal peptide, an extracellular region with cytokine receptor homology (CRH), multiple fibronectin type III domains and a WSXWS motif, a transmembrane domain, and a cytoplasmic domain that supports JAK/STAT signaling (2, 3). Mouse Leptin R shares 93% aa sequence identity with rat Leptin R and 74‑76% with human, bovine, canine, equine and porcine Leptin R. Leptin R isoforms include a long form, OB RL or OB Rb (primary signaling form), and at least four shorter isoforms with truncated cytoplasmic domains, named OB Ra (ubiquitous), Rc, Rd, and Rf (2, 4). A soluble isoform, OB Re, is found in rodents but not humans (5). However, both rodents and humans produce soluble Leptin R due to release of soluble ectodomains by metalloproteinases such as ADAM10 (5, 6). OB Rb is highly expressed in the hypothalamus and mediates the anti‑orexigenic effects of Leptin (1, 2). Mutations of ObRb have caused extreme obesity in humans, mice (db/db “diabetes”), and rats (Zucker fa/fa “fatty”) (1, 7‑9). Shorter isoforms of Leptin R exhibit limited signaling capability, but mediate endocytosis and degradation of Leptin and passage through the blood-brain barrier (4, 5, 10, 11). Soluble Leptin R is the primary Leptin‑binding protein in blood, where it maintains a pool of available bioactive Leptin, delays Leptin clearance from circulation, and down‑regulates blood‑brain transmission of Leptin (5‑7, 10). In humans, soluble Leptin R levels are inversely proportional to adiposity and are elevated in females versus males (12). Soluble Leptin R is also found up‑regulated in patients with chronic heart failure, end-stage renal disease, and anorexia (13‑15). It is expressed by tumor‑initiating stem cells, and is proposed as a link between between cancer and obesity (16).

References
  1. Israel, D. and S. Chua, Jr. (2010) Trends Endocrinol. Metab. 21:10.
  2. Oswal, A. and G. Yeo (2010) Obesity 18:221.
  3. Tartaglia, L.A. et al. (1995) Cell 83:1263.
  4. Murakami, T. et al. (1997) Biochem. Biophys. Res. Commun. 231:26.
  5. Lou, P.H. et al. (2010) PLoS ONE 5:e11669.
  6. Schaab, M. et al. (2012) PLoS ONE 7:e34787.
  7. Huang, L. et al. (2001) J. Biol. Chem. 276:6343.
  8. Chen, H. et al. (1996) Cell 84:491.
  9. Phillips, M.S. et al. (1996) Nature Genet. 13:18.
  10. Tu, H. et al. (2008) J. Cell Physiol. 214:301.
  11. Tu, H. et al. (2007) J. Cell. Physiol. 212:215.
  12. Mann, D.R. et al. (2003) J. Clin. Endocrinol. Metab. 88:3339.
  13. Schulze, P.C. et al. (2003) Eur. J. Heart Fail. 5:33.
  14. Pecoits-Filho, R. et al. (2002) Eur. J. Clin. Invest. 32:811.
  15. Krizova, J. et al. (2002) Endocr. Res. 28:199.
  16. Feldman, D.E. et al. (2012) Proc. Natl. Acad. Sci. USA 109:829.
Long Name
Leptin Receptor
Entrez Gene IDs
3953 (Human); 16847 (Mouse)
Alternate Names
B219; CD295 antigen; CD295; DB; DKFZp686B1731; huB219; LEPR; LEP-R; Leptin R; leptin receptor; LeptinR; OB R; OB receptor; OB-R; OBRCD295

FAQs

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Recombinant Mouse Leptin R Fc Chimera Protein, CF
By Anonymous on 09/07/2018
Application: Binding assay/Protein-protein interaction