Recombinant Mouse LRRTM3 Protein, CF

Catalog # Availability Size / Price Qty
6358-LR-050
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Recombinant Mouse LRRTM3 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Able to significantly enhance neurite outgrowth when immobilized as a 3 μL droplet containing 60-250 ng on a nitrocellulose coated microplate.
Source
Mouse myeloma cell line, NS0-derived mouse LRRTM3 protein
Met1-His419, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Glu31
Predicted Molecular Mass
45.3 kDa
SDS-PAGE
55-65 kDa, reducing conditons

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6358-LR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: LRRTM3

Leucine-rich repeat transmembrane neuronal 3 (LRRTM3) is a 68 kDa type I transmembrane protein that belongs to the LRRTM family, LRR superfamily of proteins (1). Mouse LRRTM3 is 582 amino acids (aa) in length with a 30 aa signal sequence, a 390 aa extracellular region, a 21 aa transmembrane region, and a 141 aa cytoplasmic domain. Within the extracellular region, there are ten LRRs flanked by cysteine-rich domains and one potential site for N‑linked glycosylation (1, 2). The cytoplasmic region contains several tyrosine, serine, and threonine residues that have potential to be phosphorylated and thus to be involved in signal transduction (1). There is also a conserved ECEV motif that interacts with PDZ proteins (1, 3). One isoform variant exists that shows an Ile514Val transition coupled to a deletion of aa 515 ‑ 582. Mature mouse LRRTM3 shares 98% aa sequence identity with mature human LRRTM3. All LRRTM proteins are expressed in the brain with varying profiles in other tissues (3). Strong LRRTM3 expression is present prenatally at 8.5 dpc in the neural progenitors of the anterior neural plate that will form the rostral neural tube (3). Expression is also seen in the region of the presomitic mesoderm/somite boundary plus the forebrain and developing eye (3). In the adult, LRRTM3 may be expressed both on neurons and placental trophoblasts. In neurons, it has been demonstrated to positively influenced by STOX1 and subsequently to induce BACE1 cleavage of APP, thus generating A beta PP beta production and a C99 fragment (2, 4).

References
  1. Lauren, J. et al. (2003) Genomics 81:411.
  2. Majercak, J. et al. (2006) Proc. Natl. Acad. Sci. USA 103:17967.
  3. Haines, B.P. and P.W.J. Rigby (2007) Gene Expr. Patterns 7:23.
  4. van Dijk, M. et al. (2010) J. Alzheimers Dis. 19:673.
Long Name
Leucine Rich Repeat Transmembrane Neuronal 3
Entrez Gene IDs
347731 (Human); 216028 (Mouse); 294380 (Rat)
Alternate Names
leucine rich repeat transmembrane neuronal 3; leucine-rich repeat transmembrane neuronal protein 3; LRRTM3; MGC131810

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reconstitution Buffers

Reviews for Recombinant Mouse LRRTM3 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Mouse LRRTM3 Protein, CF and earn rewards!

Have you used Recombinant Mouse LRRTM3 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review