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Recombinant Mouse Lymphotoxin-alpha/TNF-beta, CF

Catalog #: 749-TB/CF
3 Citations Datasheet / COA / SDS

Discontinued Product

749-TB/CF has been discontinued.
View all Lymphotoxin-alpha/TNF-beta products.
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Lymphotoxin-alpha/TNF-beta, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 0.125-0.5 µg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Lymphotoxin-alpha/TNF-beta protein
Leu34-Leu202, with an N-terminal 7-His tag
Accession # P09225
Accession #
P09225
N-terminal Sequence
Analysis
His
Predicted Molecular Mass
19.5 kDa
SDS-PAGE
25 kDa, reducing conditions

Product Datasheets

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749-TB/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

749-TB/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Lymphotoxin-alpha/TNF-beta

Tumor necrosis factor-beta (TNF-beta ), also known as lymphotoxin-alpha (LT-alpha ), is a secreted homotrimeric glycoprotein belonging to the TNF superfamily and is designated TNFSF1B. It is produced by NK, T, and B cells. TNF-beta was originally identified as protein that kills tumor cells in cell culture supernatants of a lymphoblastoid cell line. The TNF-beta subunit also associates with the type II transmembrane TNF superfamily protein lymphotoxin beta (LT beta ) to generate two types of heterotrimers designated as LT alpha 1 beta 2 (a single TNF-beta chain non-covalently associated with two chains of LT beta ), and LT alpha 2 beta 1 (1, 2). TNF-alpha, TNF-beta, and LT beta form a subfamily of the TNF related ligands. Their genes are genetically linked within a compact cluster inside the major histocompatibility complex locus (2, 3). The soluble TNF-beta binds and signals through TNF R1 and TNF R2. In contrast, the membrane-bound LT alpha 1 beta 2 interacts specifically with the LT beta receptor (LT beta R), which does not bind TNF-beta or TNF-alpha. Both TNFR1 and TNFR2 bind LT alpha 2 beta 1, which is recognized weakly by LT beta R (4, 5). TNF R1 and 2 express very broadly, while expression of LT beta  R is restricted to stromal cells of lymphoid tissues. Herpesvirus entry mediator binds TNF-beta in vitro (6). The physiological importance of such interaction, if it occurs in vivo, is unclear. Distinct functions attributed to TNF-beta from transgenic knock-out mice include, loss of lymph node development, change in splenic architecture, impaired germinal center formation, and susceptibility to pulmonary tuberculosis (7, 8). TNF-beta also has overlapping physiological functions with LT beta and TNF-alpha in lymphoid organogenesis (7). Mouse and human TNF-beta share approximately 74% homology in their amino acid sequence.

References
  1. Aggarwal, B.B. (2003) Nature Rev. Immunol. 3:745.
  2. Browning, J.L. et al. (1993) Cell 72:846.
  3. Browning, J.L. et al. (1995) J. Immunol. 154:33.
  4. Pokholok, D.K. et al. (1995) Proc. Natl. Acad. Sci. USA 92:674.
  5. Aggarwal, B.B. et al. (1985) Nature 318:665.
  6. Crowe, P.D. et al. (1994) Science 264:707.
  7. Mauri, D.N. et al. (1998) Immunity 8:21.
  8. Tumanov, A.V. et al. (2003) Cytokine & Growth Factor Rev. 14:275.
  9. Roach, D.R. et al. (2001) J. Exp. Med. 193:239.
Long Name
Tumor Necrosis Factor beta
Entrez Gene IDs
4049 (Human); 16992 (Mouse)
Alternate Names
LT; LTA; LT-alpha; lymphotoxin alpha (TNF superfamily, member 1); Lymphotoxin alpha; Lymphotoxinalpha; Lymphotoxin-alpha; tnfb; TNF-beta; TNFBlymphotoxin-alpha; TNFSF1; TNFSF1B; TNFSF1TNF-beta; tumor necrosis factor beta; Tumor necrosis factor ligand superfamily member 1

Citations for Recombinant Mouse Lymphotoxin-alpha/TNF-beta, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Regulation of innate CD8+ T-cell activation mediated by cytokines.
    Proc Natl Acad Sci U S A, 2012-06-04;109(25):9971-6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Soluble lymphotoxin is an important effector molecule in GVHD and GVL.
    Authors: Markey KA, Burman AC, Banovic T
    Blood, 2009-09-29;115(1):122-32.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA (Standard)
  3. Overexpression of lymphotoxin in T cells induces fulminant thymic involution.
    Authors: Heikenwalder M, Prinz M, Zeller N, Lang KS, Junt T, Rossi S, Tumanov A, Schmidt H, Priller J, Flatz L, Rulicke T, Macpherson AJ, Hollander GA, Nedospasov SA, Aguzzi A
    Am. J. Pathol., 2008-05-15;172(6):1555-70.
    Applications: ELISA (Standard)

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