Recombinant Mouse N-Cadherin Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
6626-NC-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse N-Cadherin Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of U‑118‑MG human glioblastoma/astrocytoma cells.

The ED50 for this effect is 0.1-0.5 μg/mL.

Source
Mouse myeloma cell line, NS0-derived mouse N-Cadherin protein
Mouse N-Cadherin
(Met1 - Ala724)
Accession # NP_031690
IEGRMDP Mouse IgG2A
(Glu98 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Asp160, Ser26, & Glu28
Predicted Molecular Mass
88.8 kDa (monomer, mature protein) & 104.2 kDa (monomer, pro-protein)
SDS-PAGE
(115-120) kDa & (130-135) kDa, reducing conditions

Product Datasheets

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6626-NC

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6626-NC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: N-Cadherin

Neuronal Cadherin (N‑Cadherin or NCAD), also known as Cadherin‑2 (CDH2), is a 130 kDa type I membrane protein belonging to the Cadherin superfamily of calcium‑dependent adhesion molecules. Cadherins are involved in multiple processes including embryonic development, cell migration, and maintenance of epithelial integrity (1, 2). Mouse N‑Cadherin is synthesized with a 25 amino acid (aa) signal peptide and a 134 aa N‑terminal propeptide. The mature cell surface‑expressed protein consists of a 565 amino acid (aa) extracellular domain (ECD) that contains five Cadherin repeats, a 21 aa transmembrane segment, and a 161 aa cytoplasmic domain (3). Within the ECD, mouse N‑Cadherin shares 98% and 99% aa sequence identity with human and rat N‑Cadherin, respectively. In the nervous system, N‑Cadherin mediates adhesion between the opposing faces of developing neuronal synapses and between Schwann cells and neuronal axons (4, 5). It interacts in cis or in trans homophilically and with the GluR2 subunit of neuronal AMPA receptors (1, 6). During synaptic maturation, its expression is lost from inhibitory terminals but maintained at excitatory terminals (5). ADAM10‑mediated shedding of the N‑Cadherin ECD alters cell‑cell adhesion, synaptic development, and AMPA receptor activity (7, 8). N‑Cadherin can also be cleaved at multiple additional sites within the intracellular or extracellular domains by Calpain, gamma ‑Secretase, and several MMPs (9 ‑ 13). Cleavage of N‑Cadherin in atherosclerotic plaques contributes alternatively to vascular smooth muscle cell proliferation (MMP‑9 and ‑12) or apoptosis (MMP‑7) (12, 13). Aberrant cell surface expression of the pro and mature forms of N‑Cadherin in cancer results in increased tumor progression and invasiveness (14, 15). N‑Cadherin also mediates the adhesion between hematopoeitic progenitor cells and mesenchymal stromal cells of the bone marrow (16).

References
  1. Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
  2. Gumbiner, B.M. (2005) Nat. Rev. Mol. Cell Biol. 6:622.
  3. Miyatani, S. et al. (1989) Science 245:631.
  4. Wanner, I.B. and P.M. Wood (2002) J. Neurosci. 22:4066.
  5. Benson, D.L. and H. Tanaka (1998) J. Neurosci. 18:6892.
  6. Saglietti, L. et al. (2007) Neuron 54:461.
  7. Reiss, K. et al. (2005) EMBO J. 24:742.
  8. Malinverno, M. et al. (2010) J. Neurosci. 30:16343.
  9. Jang, Y.-N. et al. (2009) J. Neurosci. 29:5974.
  10. Uemura, K. et al. (2006) Neurosci. Lett. 402:278.
  11. Hartland, S.N. et al. (2009) Liver Int. 29:966.
  12. Williams, H. et al. (2010) Cardiovasc. Res. 87:137.
  13. Dwivedi, A. et al. (2009) Cardiovasc. Res. 81:178.
  14. Maret, D. et al. (2010) Neoplasia 12:1066.
  15. Tanaka, H. et al. (2010) Nat. Med. 16:1414.
  16. Wein, F. et al. (2010) Stem Cell Res. 4:129.
Long Name
Neural Cadherin
Entrez Gene IDs
1000 (Human); 12558 (Mouse); 83501 (Rat)
Alternate Names
ACOGS; ARVD14; cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; calcium-dependent adhesion protein, neuronal; CD325 antigen; CD325; CDH2; CDHN; CDw325; NCAD; N-cadherin 1; NCadherin; N-Cadherin; Neural cadherin; neural-cadherin

Citations for Recombinant Mouse N-Cadherin Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Secretomics reveals gelatinase substrates at the blood-brain barrier that are implicated in astroglial barrier function
    Authors: Burmeister, M;Fraunenstein, A;Kahms, M;Arends, L;Gerwien, H;Deshpande, T;Kuhlmann, T;Gross, CC;Naik, VN;Wiendl, H;Klingauf, J;Meissner, F;Sorokin, L;
    Science advances
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. EGFR inhibition leads to enhanced desmosome assembly and cardiomyocyte cohesion via ROCK activation
    Authors: M Shoykhet, O Dervishi, P Menauer, M Hiermaier, S Moztarzade, C Osterloh, RJ Ludwig, T Williams, B Gerull, S Kaab, S Clauss, D Schüttler, J Waschke, S Yeruva
    JCI Insight, 2023-03-22;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. NELL2-Robo3 complex structure reveals mechanisms of receptor activation for axon guidance
    Authors: JS Pak, ZJ DeLoughery, J Wang, N Acharya, Y Park, A Jaworski, E Özkan
    Nat Commun, 2020-03-20;11(1):1489.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Cell-Intrinsic Control of Interneuron Migration Drives Cortical Morphogenesis
    Authors: CG Silva, E Peyre, MH Adhikari, S Tielens, S Tanco, P Van Damme, L Magno, N Krusy, G Agirman, MM Magiera, N Kessaris, B Malgrange, A Andrieux, C Janke, L Nguyen
    Cell, 2018-02-22;172(5):1063-1078.e19.
    Species: N/A
    Sample Types: Complex Sample Type
    Applications: Bioassay
  5. Auditory cortex interneuron development requires cadherins operating hair-cell mechanoelectrical transduction
    Authors: B Libé-Phili, V Michel, J Boutet de, S Le Gal, T Dupont, P Avan, C Métin, N Michalski, C Petit
    Proc. Natl. Acad. Sci. U.S.A., 2017-07-13;114(30):7765-7774.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  6. Matrix metalloproteinase activity stimulates N-cadherin shedding and the soluble N-cadherin ectodomain promotes classical microglial activation
    Authors: K Conant, S Daniele, PL Bozzelli, T Abdi, A Edwards, A Szklarczyk, I Olchefske, D Ottenheime, K Maguire-Ze
    J Neuroinflammation, 2017-03-17;14(1):56.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  7. Reelin transiently promotes N-cadherin-dependent neuronal adhesion during mouse cortical development
    Authors: Y Matsunaga, M Noda, H Murakawa, K Hayashi, A Nagasaka, S Inoue, T Miyata, T Miura, KI Kubo, K Nakajima
    Proc. Natl. Acad. Sci. U.S.A, 2017-02-07;114(8):2048-2053.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Nanoscale architecture of cadherin-based cell�adhesions
    Nat. Cell Biol, 2016-12-19;19(1):28-37.
    Applications: Bioassay
  9. Neural cell-cell and cell-substrate adhesion through N-cadherin, N-CAM and L1.
    Authors: Wiertz RW, Marani E, Rutten WL
    J Neural Eng, 2011-05-31;8(4):046004.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay

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