Recombinant Mouse Neuregulin-1/NRG1 Protein, CF

Catalog # Availability Size / Price Qty
9875-NR-050
Recombinant Mouse Neuregulin-1/NRG1 Protein Bioactivity
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Citations (5)
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Recombinant Mouse Neuregulin-1/NRG1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 0.9-5.4 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Neuregulin-1/NRG1 protein
Ser20-Lys246, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ser20
Predicted Molecular Mass
26 kDa
SDS-PAGE
42-49 kDa, reducing conditions

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Product Datasheets

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9875-NR

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9875-NR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Recombinant Mouse Neuregulin-1/NRG1 Protein Bioactivity View Larger

Recombinant Mouse Neuregulin-1/NRG1 (Catalog # 9875-NR) stimulates MCF-7 human breast cancer cell proliferation. The ED50 for this effect is 0.9-5.4 ng/mL.

SDS-PAGE Recombinant Mouse Neuregulin-1/NRG1 Protein SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse Neuregulin-1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 42-49 kDa.

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Background: Neuregulin-1/NRG1

Neuregulin-1 (NRG1) belongs to a family of structurally related glycoproteins encoded by four distinct but related genes, Nrg1, Nrg2, Nrg3, and Nrg4. In humans, Nrg1 encodes multiple soluble or transmembrane proteins through alternative splicing or the use of alternative promoters with each NRG1 isoform exhibiting distinct expression patterns and functions (1). Type I isoforms include Neu Differentiation Factor, Heregulin, and ARIA, and they consist of an N-terminal domain, an Ig-like domain, a linker with a Ser/Thr rich region, an EGF-like domain, a transmembrane segment, and a cytoplasmic domain. Type II isoforms have a larger N-terminal domain and lack the Ser/Thr rich linker, while Type III isoforms lack the Ig-like domain but contain a cysteine rich domain (CRD) and a second transmembrane segment (1-6). The mature mouse NRG1 isoform expressed corresponds to Heregulin-beta 1 with an extracellular domain of 229 amino acids (aa) that shares 92% and 79% sequence identity with human and rat NRG1, respectively. NRG1 regulates multiple nervous system functions including axon guidance, synapse formation and plasticity, glial cell development, and axon myelination (1-3). All NRG1 isoforms share an EGF-like domain that interacts with the ErbB family of tyrosine kinase receptors to induce signaling (1-4). Heregulin-beta 1 has been directly linked to diverse functions including cardiovascular development and protection from ischemic injury (7) to a role in the growth and maintenance of human embryonic stem cells (8). Multiple polymorphisms and aberrant expression of NRG1 isoforms are associated with the development of schizophrenia and several cancers (2, 9, 10). Activation of Heregulin-beta 1 signaling has been shown to promote invasiveness and metastasis of breast cancer cells (11, 12).

References
  1. Meyer, D. et al. (1997) Development 124:3575.
  2. Mei, L. and W.-C. Xiong (2008) Nat. Rev. Neurosci. 9:437.
  3. Talmage, D.A. (2008) Novartis Found. Symp. 289:74.
  4. Holmes, W.E. et al. (1992) Science 256:1205.
  5. Marchionni, M.A. et al. (1993) Nature 362:312.
  6. Ho, W.-H. et al. (1995) J. Biol. Chem. 270:14523.
  7. Odiete et al. (2012) Circ Res. 111(10):1376.
  8. Wang, L. et al. (2007) Blood. Dec 1; 110(12):4111.
  9. Harrison PJ and Law AJ. (2006) Biol Psychiatry 60(2):132.
  10. Breleux, M. (2007) Cell. Mol. Life Sci. 64:2358.
  11. Atlas, E. et al. (2003) Mol Cancer Res. 2003(3):165.
  12. Hutcheson IR et al. (2007) Breast Cancer Res. 9(4):R50.
Entrez Gene IDs
3084 (Human); 211323 (Mouse); 112400 (Rat)
Alternate Names
ARIA; GGF2; GGFglial growth factor; Heregulin-1; HGL; HGLneu differentiation factor; HRG; HRG1; HRG1-alpha; HRG1-beta 1; HRGAneuregulin 1 type IV beta 1a; MST131; MSTP131; NDFheregulin, alpha (45kD, ERBB2 p185-activator); neuregulin 1 type IV beta 3; neuregulin 1; Neuregulin1; Neuregulin-1; NRG1; pro-neuregulin-1, membrane-bound isoform; pro-NRG1; sensory and motor neuron derived factor; SMDF

Citations for Recombinant Mouse Neuregulin-1/NRG1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Neuregulin-1 signaling regulates cytokines and chemokines expression and secretion in granulosa cell
    Authors: S Banerjee, S Mishra, W Xu, WE Thompson, I Chowdhury
    Journal of ovarian research, 2022;15(1):86.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Epithelium-derived Indian Hedgehog restricts stromal expression of ErbB family members that drive colonic tumor cell proliferation
    Authors: F Westendorp, ON Karpus, PJ Koelink, JLM Vermeulen, S Meisner, J Koster, NVJA Büller, ME Wildenberg, V Muncan, GR van den Br
    Oncogene, 2021;0(0):.
    Species: Transgenic Mouse
    Sample Types: Organoid
    Applications: Bioassay
  3. Thrombomodulin facilitates peripheral nerve regeneration through regulating M1/M2 switching
    Authors: TC Huang, HL Wu, SH Chen, YT Wang, CC Wu
    J Neuroinflammation, 2020;17(1):240.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Injectable hydrogels of optimized acellular nerve for injection in the injured spinal cord
    Authors: RC Cornelison, E Gonzalez-R, SL Porvasnik, SM Wellman, JH Park, DD Fuller, CE Schmidt
    Biomed Mater, 2018;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Cell Culture
  5. Class IIa histone deacetylases link cAMP signaling to the myelin transcriptional program of Schwann cells
    Authors: C Gomis-Colo, S Velasco-Av, JA Gomez-Sanc, A Casillas-B, J Backs, H Cabedo
    J. Cell Biol., 2018;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay

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