Recombinant Mouse Periostin/OSF-2 Protein, CF

Catalog # Availability Size / Price Qty
2955-F2-050

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Recombinant Mouse Periostin/OSF-2 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. Immobilized Recombinant Mouse Periostin/OSF‑2 at 10 µg/mL (100 µL/well) induces >50% cell adhesion.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse Periostin/OSF-2 protein
Asn24-Gln811, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Asn24
Predicted Molecular Mass
88.5 kDa
SDS-PAGE
90 kDa, reducing conditions

Product Datasheets

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2955-F2

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2955-F2

Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Periostin/OSF-2

Periostin, also known as OSF-2, is a secreted matricellular protein with functions in extracellular matrix formation, cell migration, and inflammation (1). It is secreted as a 90 kDa monomer that can aggregate into >170 kDa higher-order multimers (2). Periostin contains an N-terminal EMI domain followed by four tandem FAS1 domains (3). Mature mouse Periostin shares 91% and 98% aa sequence identity with mouse and rat Periostin, respectively. Alternative splicing generates additional isoforms with various deletions in the C-terminal region following the FAS domains. Periostin is expressed by mesenchymal cells such as vascular smooth muscle cells, fibroblasts, osteoblasts, and odontoblasts in developing teeth (4-7). It is up-regulated in many carcinomas (2, 8). Periostin binds to Integrins alpha v beta 3 and alpha v beta 5 (2, 9), leading to enhanced cell adhesion and cell migration (2, 5, 6). It enhances Fibronectin and Collagen I production and promotes collagen fibrillogenesis (10, 11). It also induces epithelial-mesenchymal transition, tumor growth, invasion, and metastasis (9). Periostin induces the expression of VEGF R2 on endothelial cells and VEGF-C in tumor cells, and it can induce tumor lymphangiogenesis (8, 12). Periostin plays an important role in heart valve development and tissue healing after myocardial infarction (5, 13, 14). In asthma, it is upregulated in bronchial epithelium and plays both destructive and protective roles by inducing eosinophil infiltration and inhibiting goblet cell metaplasia and mucus production, respectively (15, 16).

References
  1. Liu, A.Y. et al. (2014) Matrix Biol. 37:150.
  2. Gillan, L. et al. (2002) Cancer Res. 62:5358.
  3. Takeshita, S. et al. (1993) Biochem. J. 294:271.
  4. Kruzynska-Frejtag, A. et al. (2004) Dev. Dyn. 229:857.
  5. Lindner, V. et al. (2005) Arterioscler. Thromb. Vasc. Biol. 25:77.
  6. Horiuchi, K. et al. (1999) J. Bone Miner. Res. 14:1239.
  7. Li, G. et al. (2006) Atherosclerosis 188:292.
  8. Shao, R. et al. (2004) Mol. Cell. Biol. 24:3992.
  9. Yan, W. and R. Shao (2006) J. Biol. Chem. 281:19700.
  10. Erkan, M. et al. (2007) Gastroenterology 132:1447.
  11. Norris, R.A. et al. (2007) J. Cell. Biochem. 101:695.
  12. Kudo, Y. et al. (2012) PLoS One 7:e44488.
  13. Snider, P. et al. (2008) Circ. Res. 102:752.
  14. Kuhn, B. et al. (2007) Nat. Med. 13:962.
  15. Blanchard, C. et al. (2008) Mucosal Immunol. 1:289.
  16. Sehra, S. et al. (2011) J. Immunol. 186:4959.
Long Name
Osteoblast Specific Factor 2
Entrez Gene IDs
10631 (Human); 50706 (Mouse); 361945 (Rat)
Alternate Names
Fasciclin I-like; MGC119510; MGC119511; OSF2; OSF-2; OSF-2osteoblast specific factor 2 (fasciclin I-like); OSF2periodontal ligament-specific periostin; Osteoblast-specific factor 2; PDLPOSTN; periostin isoform thy2; periostin isoform thy4; periostin isoform thy6; periostin isoform thy8; Periostin; periostin, osteoblast specific factor; PNRP11-412K4.1; POSTN; TRIF52

Citations for Recombinant Mouse Periostin/OSF-2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Identification of Periostin as a critical niche for myofibroblast dynamics and fibrosis during tendon healing
    Authors: Ackerman, JE;Adjei-Sowah, E;Korcari, A;Muscat, SN;Nichols, AEC;Buckley, MR;Loiselle, AE;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Periostin deficiency reduces PD-1+ tumor-associated macrophage infiltration and enhances anti-PD-1 efficacy in colorectal cancer
    Authors: T Wei, K Wang, S Liu, Y Fang, Z Hong, Y Liu, H Zhang, C Yang, G Ouyang, T Wu
    Cell Reports, 2023-02-09;42(2):112090.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Periosteal stem cells control growth plate stem cells during postnatal skeletal growth
    Authors: M Tsukasaki, N Komatsu, T Negishi-Ko, NC Huynh, R Muro, Y Ando, Y Seki, A Terashima, W Pluemsakun, T Nitta, T Nakamura, T Nakashima, S Ohba, H Akiyama, K Okamoto, R Baron, H Takayanagi
    Oncogene, 2022-07-18;13(1):4166.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Periostin Short Fragment with Exon 17 via Aberrant Alternative Splicing Is Required for Breast Cancer Growth and Metastasis
    Authors: Y Ikeda-Iwab, Y Taniyama, N Katsuragi, F Sanada, N Koibuchi, K Shibata, K Shimazu, H Rakugi, R Morishita
    Cells, 2021-04-14;10(4):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Surface Plasmon Resonance
  5. FAM20C directly binds to and phosphorylates Periostin
    Authors: JH Lin, IP Lin, Y Ohyama, H Mochida, A Kudo, M Kaku, Y Mochida
    Sci Rep, 2020-10-13;10(1):17155.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Periostin Activation of Integrin Receptors on Sensory Neurons Induces Allergic Itch
    Authors: SK Mishra, JJ Wheeler, S Pitake, H Ding, C Jiang, T Fukuyama, JS Paps, P Ralph, J Coyne, M Parkington, J DeBrecht, LC Ehrhardt-H, GP Cruse, W Bäumer, RR Ji, MC Ko, T Olivry
    Cell Rep, 2020-04-07;31(1):107472.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  7. Periostin Promotes Colorectal Tumorigenesis through Integrin-FAK-Src Pathway-Mediated YAP/TAZ Activation
    Authors: H Ma, J Wang, X Zhao, T Wu, Z Huang, D Chen, Y Liu, G Ouyang
    Cell Rep, 2020-01-21;30(3):793-806.e6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Macrophage-lineage TRAP+ cells recruit periosteum-derived cells for periosteal osteogenesis and regeneration
    Authors: B Gao, R Deng, Y Chai, H Chen, B Hu, X Wang, S Zhu, Y Cao, S Ni, M Wan, L Yang, Z Luo, X Cao
    J. Clin. Invest., 2019-04-04;129(6):2578-2594.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  9. Bone Marrow Mesenchymal Stromal Cell-Derived Periostin Promotes B-ALL Progression by Modulating CCL2 in Leukemia Cells
    Authors: Z Ma, X Zhao, M Deng, Z Huang, J Wang, Y Wu, D Cui, Y Liu, R Liu, G Ouyang
    Cell Rep, 2019-02-05;26(6):1533-1543.e4.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Identification of genes which regulate stroma-dependent in vitro hematopoiesis
    Authors: P Periasamy, V Tran, HC O'Neill
    PLoS ONE, 2018-10-11;13(10):e0205583.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Loss of periostin ameliorates adipose tissue inflammation and fibrosis in vivo
    Authors: F Nakazeki, M Nishiga, T Horie, H Nishi, Y Nakashima, O Baba, Y Kuwabara, T Nishino, T Nakao, Y Ide, S Koyama, M Kimura, S Tsuji, N Sowa, S Yoshida, SJ Conway, M Yanagita, T Kimura, K Ono
    Sci Rep, 2018-06-04;8(1):8553.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Periostin attenuates tumor growth by inducing apoptosis in colitis-related colorectal cancer
    Authors: Y Shimoyama, K Tamai, R Shibuya, M Nakamura, M Mochizuki, K Yamaguchi, Y Kakuta, Y Kinouchi, I Sato, A Kudo, T Shimosegaw, K Satoh
    Oncotarget, 2018-04-13;9(28):20008-20017.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Periostin Is a Key Niche Component for Wound Metastasis of Melanoma.
    Authors: Fukuda K, Sugihara E, Ohta S, Izuhara K, Funakoshi T, Amagai M, Saya H
    PLoS ONE, 2015-06-17;10(6):e0129704.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Histamine contributes to tissue remodeling via periostin expression.
    Authors: Yang, Lingli, Murota, Hiroyuki, Serada, Satoshi, Fujimoto, Minoru, Kudo, Akira, Naka, Tetsuji, Katayama, Ichiro
    J Invest Dermatol, 2014-02-27;134(8):2105-13.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Periostin controls keratinocyte proliferation and differentiation by interacting with the paracrine IL-1alpha/IL-6 loop.
    Authors: Taniguchi K, Arima K, Masuoka M, Ohta S, Shiraishi H, Ontsuka K, Suzuki S, Inamitsu M, Yamamoto K, Simmons O, Toda S, Conway S, Hamasaki Y, Izuhara K
    J Invest Dermatol, 2013-11-25;134(5):1295-304.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Periostin links mechanical strain to inflammation in abdominal aortic aneurysm.
    Authors: Yamashita, Osamu, Yoshimura, Koichi, Nagasawa, Ayako, Ueda, Koshiro, Morikage, Noriyasu, Ikeda, Yasuhiro, Hamano, Kimikazu
    PLoS ONE, 2013-11-19;8(11):e79753.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Periostin regulates goblet cell metaplasia in a model of allergic airway inflammation.
    Authors: Sehra S, Yao W, Nguyen ET, Ahyi AN, Tuana FM, Ahlfeld SK, Snider P, Tepper RS, Petrache I, Conway SJ, Kaplan MH
    J. Immunol., 2011-03-14;186(8):4959-66.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Mouse Periostin/OSF-2 Protein, CF
By Anonymous on 09/01/2016
Application: In vitro bioactivity in cell culture