Recombinant Mouse PLA2R1 Protein, CF

R&D Systems | Catalog # 5367-PL

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse PLA2R1 Protein (5367-PL)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

NP_032893

Applications

Bioactivity
View all PLA2R1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse PLA2R1 protein
Gln27-Gly1392, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln27 predicted (N-sequencing might be blocked)

Predicted Molecular Mass

157.5 kDa

SDS-PAGE

150-170 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.

When Recombinant Human PLA2G1B (Catalog # 5018-PL) is immobilized at 5 μg/mL (100 μL/well), the concentration of Recombinant Mouse PLA2R1 that produces 50% of the optimal binding response is found to be approximately 1.5-7.5 μg/mL.

Formulation, Preparation, and Storage

5367-PL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: PLA2R1

PLA2R1 (phospholipase A2 receptor 1) also called muscle (M-)type PLA2R or secretory (s)PLA2R is a 180-200 kDa type I transmembrane glycoprotein that is a member of subgroup VI of the C-type lectin superfamily (1-3). The 1487 amino acid (aa) mouse PLA2R1 contains a 26 aa signal sequence, a 1370 aa ECD, a 21 aa transmembrane sequence, and a 70 aa cytoplasmic domain with an endocytosis motif. The ECD contains one ricin B-type lectin domain (aa 42-165), a fibronectin type II region (aa 176-224), and eight C-type lectin carbohydrate recognition domains (CRDs, aa 241-1377) (1-3). CRDs 3-5 are the major PLA2 binding site (1, 3). The mouse PLA2R1 ECD shares 75% and 86% aa identity with human and rat PLA2R1, respectively. One isoform shows a 35 aa N-terminal extension, and a potentially secreted isoform contains a 10 aa substitution for aa 680-1487 (4). Matrix metalloproteinases can generate a 180 kDa soluble inhibitory form in mouse serum that retains PLA2 binding activity (3, 5). Mouse PLA2R1 is widely expressed, including on type II alveolar cells, neutrophils and mast cells, and binds mouse secretory PLA2s IB, IIA, IIE, IIF and X, and snake venom PLAs (1-3, 5-8). Among mammals, PLA2R1 ligand recognition is mainly species‑specific (3). PLA2R1 can be anti‑inflammatory by inhibiting PLA2‑induced release lipid mediators such as arachadonic acid (5  7, 9). PLA2R1 can also mediate internalization and clearance of PLA2s (10). PLA2R1 can also be pro‑inflammatory, and its deletion in mouse reduces susceptibility to LPS-induced endotoxic shock (8). Engagement activates the MAPK/ERK signaling pathway and enhances pro-inflammatory cytokine release (6, 8, 11). PLA2 binding can stimulate cell adhesion, proliferation or senescence, neutrophil elastase release, and smooth muscle contraction (6, 9, 12). All PLA2R1 intracellular signaling is largely independent of ligand phospholipase activity (6, 11).

References

  1. Higashino, K-I. et al. (1994) Eur. J. Biochem. 225:375.
  2. Lambeau, G. et al. (1994) J. Biol. Chem. 269:1575.
  3. Hanasaki, K. (2004) Biol. Pharm. Bull. 27:1165.
  4. SwissProt Accession Q62028.
  5. Higashino, K-I. et al. (2002) J. Biol. Chem. 277:13583.
  6. Silliman, C.C. et al. (2002) Am. J. Physiol. Cell Physiol. 283:C1102.
  7. Rouault, M. et al. (2007) Biochemistry 46:1647.
  8. Hanasaki, K. et al. (1997) Biol. Chem. 272:32792.
  9. Ancian, P. et al. (1995) Biochemistry 34:13146.
  10. Yokota, Y. et al. (2001) FEBS Lett. 509:250.
  11. Granata, F. et al. (2005) J. Immunol. 174:464.
  12. Augert, A. et al. (2009) EMBO Rep. 10:271.

Long Name

Phospholipase A2 Receptor 1, 180kDa

Alternate Names

CLEC13C, PLA2G1R

Entrez Gene IDs

22925 (Human); 18779 (Mouse); 295631 (Rat)

Gene Symbol

PLA2R1

UniProt

NP_032893

Additional PLA2R1 Products

Product Documents for Recombinant Mouse PLA2R1 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Mouse PLA2R1 Protein, CF

For research use only

Citations for Recombinant Mouse PLA2R1 Protein, CF

Customer Reviews for Recombinant Mouse PLA2R1 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Mouse PLA2R1 Protein, CF and earn rewards!

Have you used Recombinant Mouse PLA2R1 Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes