Recombinant Mouse Pref-1/DLK-1/FA1 Protein, CF

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Recombinant Mouse Pref-1/DLK-1/FA1 Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE with silver staining
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to inhibit adipocyte differentiation of mouse 3T3‑L1 mouse embryonic fibroblast adipose-like cells. Smas, C.M. et al. (1993) Cell 73:725. Recombinant Mouse Pref‑1/DLK1/FA1 at 2.5 μg/mL will inhibit adipocyte differentiation by more than 50%.
Mouse myeloma cell line, NS0-derived mouse Pref-1/DLK1/FA1 protein
Ala24-Gln305, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
31 kDa
49-66 kDa, reducing conditions

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Pref-1/DLK1/FA1

Mouse Pref-1 (preadipocyte factor 1), also known as Dlk-1 or FA1, belongs to the Notch/Delta/Serrate family of EGF-like repeat-containing proteins that act as ligands in the Notch signaling pathway (1-3). Mouse Pref-1 is synthesized as a 385 amino acid (aa) precursor consisting of a 23 aa signal sequence, a 282 aa extracellular region, a 24 aa transmembrane segment, and a 56 aa cytoplasmic tail. The extracellular region contains six tandem EGF-like repeats, a juxtamembrane region, and multiple sites for O- and N-glycosylation. Heterogeneous transmembrane forms of Pref-1, ranging from 50 to 60 kDa, can be attributed to variability in glycosylation (2-4). In addition to full-length Pref-1A, alternative splicing of the juxtamembrane and EGF-like repeat regions generates three major short forms, Pref-1B, -1C, and
-1D (5). Proteolytic cleavage at one of two extracellular sites in Pref-1A and 1B can produce two soluble forms: a 50 kDa large form and a 24-25 kDa small form (2, 5, 6). Processing of Pref-1C and Pref-1D, by contrast, can produce only small soluble forms. Only the large soluble form demonstrates biological activity (5). The ECD of mature mouse Pref-1 shares 94% and 83% aa sequence identity with the ECD of human and rat Pref-1, respectively. Pref-1 is widely expressed during embryogenesis (2). In adults, Pref-1 expression is restricted to preadipocytes, pancreatic beta  cells, thymic stromal cells, and cells of the adrenal and pituitary glands
(1, 7-9). Tumors of neuroendocrine origin have been found to express Pref-1 (10). Down-regulation of Pref-1 in preadipocytes is associated with differentiation into mature adipocytes (1-7). Pref-1 also regulates the differentiation of skeletal stem cells, thymocytes, and adrenal gland cells and inhibits hormone secretion in pituitary cells (1). Pref-1 has also been shown to inhibit preadipocyte proliferation (11, 12).

  1. Ansell, P.J. et al. (2007) Mol. Cell. Endocrinol. 271:55.
  2. Smas, C.M. and H.S. Sul (1993) Cell 73:725.
  3. Sul, H.S. (2009) Mol. Endocrinol. 23:1717.
  4. Smas, C.M. et al. (1994) Biochemistry 33:9257.
  5. Mei, B. et al. (2002) Biochem. J. 364:137.
  6. Wang, Y. and H.S. Sul (2006) Mol. Cell. Biol. 26:5421.
  7. Wang, Y. et al. (2010) Mol. Cell. Biol. 30:3480.
  8. Friedrichsen, B.N. et al. (2003) J. Endocrinol. 176:257.
  9. Hedlund, G.P. et al. (2003) In Vivo 17:1.
  10. Yin, D. et al. (2006) Oncogene 25:1852.
  11. Traustadottir, G.A. et al. (2013) Adipocyte 2:272.
  12. Mortensen, S.B. et al. (2012) Diabetes 61:2814.
Long Name
Preadipocyte Factor-1/Protein delta Homolog 1/Fetal Antigen 1
Entrez Gene IDs
8788 (Human); 13386 (Mouse)
Alternate Names
delta-like 1 homolog (Drosophila); DLK; DLK1; DLK-1; FA1; FA1fetal antigen 1; pG2; pG2delta-like homolog (Drosophila); preadipocyte factor 1; Pref1; Pref-1; protein delta homolog 1; secredeltin; ZOG


  1. What isoform is Recombinant Mouse Pref-1/DLK-1/FA1 Protein, CF (Catalog # 8545-PR) and what is the expected molecular weight?

    • This protein corresponds to the extracellular portion of Isoform A (Pref-1A). Isoforms B-D are short form isoforms generated from alternative splices of the immunogen sequence. This protein has an amino acid sequence of Ala24-Gln305 of Accession # Q09163, with a C-terminal 6-His tag and corresponds to the sequence of Isoform A. This sequence information can be found on UniProt under “Sequence” ( We would expect the protein to have a molecular weight between 49-66 kDa under reducing conditions.

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By Anonymous on 05/23/2017
Application: Stem/Immune cell maintenance or differentiation